提供形式：in 3.2M ammonium sulphate。
酶活定义：One Unit of acetyl-CoA synthetase activity is defined as the amount of enzyme required to produce one µmole of acetyl coenzyme A from acetic acid, ATP and coenzyme A per min in the presence of NAD+ in TEA buffer (128 mM), pH 8.4 at 25℃。
催化反应： Catalyses the reaction:ATP + acetate + CoA = AMP + diphosphate + acetyl-CoA
最佳温度：37℃
最佳pH:8.4
在醋酸的分析和测量中的应用。