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  2. Human Fetuin B PicoKine ELISA Kit

Human Fetuin B PicoKine ELISA Kit

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BOSTER
武汉
2021-09-22 23:11
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产品属性
数量现货
供应商博士德生物
检测方法酶联免疫双抗夹心法
应用cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).
适应物种Human
规格96T
产品说明

产品概况

货号EK1498
产品名称Human Fetuin B PicoKine ELISA Kit
产品描述For quantitative detection of human Fetuin B in cell culture supernates, cell lysates, serum and plasma (heparin, EDTA).
免疫原NSO, C16-P382
交叉反应There is no detectable cross-reactivity with other relevant proteins.
敏感性<12pg/ml
检测范围156pg/ml-10000pg/ml
基因名称FETUB
基因全名fetuin B
Uniprot IDQ9UGM5
指标别名16G2 | D17980 | Fet | Fetub | Fetuin beta | Fetuin-B | Fetuin-like protein | Gugu | IRL685 | Pp63 | Q9UGM5
背景资料Fetuin-B is a protein that in humans is encoded by the FETUB gene. It is mapped to 3q27, where the FETUA gene is localized. The protein encoded by this gene is a member of the fetuin family, part of the cystatin superfamily of cysteine protease inhibitors. Fetuins have been implicated in several diverse functions, including osteogenesis and bone resorption, regulation of the insulin and hepatocyte growth factor receptors, and response to systemic inflammation. This protein may be secreted by cells.
显色时间15-20

试剂盒内容

SKU名称数量
Catalog numberDescriptionQuantity
EK1498-CAP96-well plate precoated with anti- human Fetuin B antibody1
EK1498-STLyophilized recombinant human Fetuin B standard10ng/tube×2
EK1498-DABiotinylated anti- human Fetuin B antibody130μl(dilution 1:100)
AR1103Avidin-Biotin-Peroxidase Complex (ABC)130μl(dilution 1:100)
AR1106-1Sample diluent buffer30ml
AR1106-2Antibody diluent buffer12ml
AR1106-3ABC diluent buffer12ml
AR1104TMB color developing agent10ml
AR1105TMB stop solution10ml
PLA-SEAAdhesive cover4
*小贴士：额外的试剂盒部件可以单独购买。如需购买请和试剂盒一起下单避免额外运费。

需要用户自己提供的器材和试剂

  • 1.96孔读板仪器。
  • 2.自动洗板机.
  • 3.移液器和枪头。建议使用多通道移液器/排枪。
  • 4.Eppendorf 管。
  • 5.额外洗板缓冲液 (neutral PBS 或 TBS。试剂盒内提供足量。如需额外缓冲液可参考如下配方)。
    • 0.01M TBS: Add 1.2g Tris, 8.5g Nacl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2O and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
    • 0.01M PBS: Add 8.5g sodium chloride, 1.4g Na2HPO4 and 0.2g NaH2PO4 to 1000ml distilled water and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
*样品准备的相关试剂也不含在试剂盒中。

批内差/批间差

[intra_inter_assay_variability]
Intra-Assay PrecisionInter-Assay Precision

Human Fetuin B PicoKine ELISA Kit的典型数据示例（取自最近的一批次质检数据）

(TMB 孵育条件： 37°C， 15-20)
Concentration(pg/ml)062.5125250500100020004000
O.D.0.0300.0620.1130.2050.4580.8281.7432.270
*典型数据仅供参考。用户需要自己建立标准曲线。
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