Blood vessels of the skin are composed of a number of cellular and non-cellular elements. The
predominant cell type in all skin blood vessels is the endothelial cell. Endothelial cells form the
interface between intravascular and extravascular compartments and serve as a selective barrier
for the diffusion of cells and macromolecules between these two compartments. The endothelial
cells lining blood vessels of the skin share features of endothelial cells elsewhere in the body, but
they also exhibit several skin specific characteristics. They are active participants in a variety of
physiological processes. These include wound healing, control of hemostasis, temperature regulation,
and the modulation of inflammation/leukocyte trafficking [1, 2]. The structure and function of
endothelial cells are differentially regulated in space and time [3]. Endothelial cell proliferation,
quiescence, apoptosis, and senescence are the four principal cytologic states that set the cutaneous
microvascular in a dynamic balance between maintenance and remodeling [4].

HDMEC from ScienCell Research Laboratories are isolated from human dermal tissue. HDMEC are
cryopreserved on passage one and delivered frozen. Each vial contains >5 x 10^5 cells in 1 ml volume.
HDMEC are characterized by immunofluorescent method with antibodies to vWF/Factor VIII and
CD31 (P-CAM) and by uptake of DiI-Ac-LDL. HDMEC are negative for HIV-1, HBV, HCV, mycoplasma,
bacteria, yeast and fungi. HDMEC are guaranteed to further expand for 15 population doublings in
the conditions provided by ScienCell Research Laboratories.

Recommended Medium
It is recommended to use Endothelial Cell Medium (ECM, Cat. No. 1001) for the culturing of HDMEC.

Product Use
HDMEC are for research use only. It is not approved for human or animal use, or for application in in

vitro diagnostic procedures.

Storage
Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keep the

cells in liquid nitrogen until cell culture needed for experiments.

Shipping
Dry ice.

Reference

 [1] Swerlick, R. A and Lawley, T. J. (1993) Role of microvascular endothelial cells in inflammation.
J. Invest. Dermatol. 100:111S.
[2] Springer, T. (1994) Traffic signals for lymphocyte recirculation and leukocyte emigration.
Cell 76:301.
[3] Aird, W. C. (2003) Endothelial cell heterogeneity. Crit. Care Med. 31(4):S221-30.
[4] Chang, E., Yang, J., Nagavarapu, U. and Herron, S. (2002) Aging and survival of cutaneous
microvasculature. J. Invest. Dermatol. 118:752.

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