产品概况
| 产品名称 | PCNA Antibody |
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| 产品描述 | Anti-PCNA Antibody validated for IHC, WB, FC, ELISA in Human, Monkey. |
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| 文献引用格式 | PCNA Antibody (Boster Biological Technology, Wuhan, China. Catalog #BM0105) |
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| 应用范围 | IHC, WB, FC, ELISA |
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| 抗体来源 | Mouse |
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| 抗体亚型 | IgG1 |
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| 免疫原 | Purified recombinant fragment of human PCNA (AA: 53-196 ) expressed in E. Coli. |
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| 抗体规格 | 200ul/1180元 |
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产品详情
| 克隆 | Monoclonal |
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| 克隆号 | 单克隆抗体：IgG1；克隆号：7H4F8 |
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| 产品形态 | Liquid |
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| 内容 | Ascitic fluid containing 0.03% sodium azide. |
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| 储存条件 | 连续使用时4℃存储，保质期六个月；长期存储时建议分装为10ul以上小包装-20℃存储，并避免反复冻融，保质期一年。 |
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指标相关信息 (来源: Uniprot.org)
小贴士：你可以利用tissue specificity信息来帮助设计阳性和阴性对照。对此有疑问请联系我们技术服务部门。
| 基因名 | PCNA |
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| 基因全名 | Proliferating cell nuclear antigen |
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| Tissue Specificity | Auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand. Induces a robust stimulatory effect on the 3'- 5' exonuclease and 3'-phosphodiesterase, but not apurinic- apyrimidinic (AP) endonuclease, APEX2 activities. Has to be loaded onto DNA in order to be able to stimulate APEX2. Plays a key role in DNA damage response (DDR) by being conveniently positioned at the replication fork to coordinate DNA replication with DNA repair and DNA damage tolerance pathways (PubMed:24939902). Acts as a loading platform to recruit DDR proteins that allow completion of DNA replication after DNA damage and promote postreplication repair: Monoubiquitinated PCNA leads to recruitment of translesion (TLS) polymerases, while 'Lys-63'-linked polyubiquitination of PCNA is involved in error-free pathway and employs recombination mechanisms to synthesize across the lesion. . |
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| Sequence Similarities | Proliferating cell nuclear antigen;PCNA;Cyclin;PCNA; |
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| Uniprot ID | PCNA: P12004 |
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指标背景简介
The protein encoded by this gene is found in the nucleus and is a cofactor of DNA polymerase delta. The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for this gene. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome.
产品应用细节
为了提供最优质的抗体，博士德对每一批抗体都用没有转染过的细胞系和体细胞组织检测，以保证博士德出品的抗体有足够的亲和性足以和对应蛋白天然的表达含量起反应。
| 应用 | 稀释度* |
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| Western blotting | 1:100-400 |
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| Immunohistochemistry in paraffin section | 1:50-200 |
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| Immunocytochemistry in fixed cells | 1:50-200 |
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| Flow Cytometry | 1:40-80 |
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| enzyme-linked immuno sorbent assay | 1:2000 |
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*最优稀释度需要用户自己调试，此处数据仅供参考。
**博士德提供高敏感的二抗和检测试剂盒。详情见相关产品推荐。
产品图片
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[list_product_images]PCNA(BM0105)(MW:29KD)A431，HeLa，HepG2细胞裂解，免疫印迹分析。|Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using PCNA mouse mAb with DAB staining.|Immunohistochemical analysis of paraffin-embedded colon cancer tissues using PCNA mouse mAb with DAB staining.|Flow cytometric analysis of MOLT4 cells using PCNA mouse mAb (green) and negative control (purple).|Black line: Control Antigen (100 ng);Purple line: Antigen(10ng);Blue line: Antigen (50 ng);Red line: Antigen (100 ng);[/list_product_images]
