Derived from the mesoderm, connective tissue is involved in maintaining the structural integrity of the body. The predominant cells found in connective tissue are fibroblasts, which continuously secrete various components of the extracellular matrix. Depending on the site of origin and physiological status, fibroblasts may exhibit different morphological phenotypes and various functional properties.AcceGen offers a range of Human Fibroblasts produced at AcceGen's cell culture facility. The cells are isolated from normal human juvenile foreskin or adult skin from different locations. In addition, AcceGen offers Fibroblasts from adult lung tissue, aortic adventitial tissue, and villous placental tissue.Shortly after isolation, all AcceGen Human Fibroblasts are cryopreserved at passage 2 (P2) using AcceGen's proprietary, serum-free freezing medium,Cryo-SFM. Each cryo vial contains more than 500,000 viable cells after thawing. Proliferating cell cultures are made from 500,000 cryopreserved cells that have been thawed and cultured for three days at AcceGen.