The C2C12 cell line differentiates rapidly, forming contractile myotubes and producing characteristic muscle proteins.Treatment with bone morphogenic protein 2(BMP-2) cause a shift in the differentiation pathway from myoblastic to osteoblastic.Tested and found negative for ectromelia virus(mousepox).Species:MouseApplications:Transfection host.Tissue:MuscleMorphology:MyoblastGrowth Properties:AdherentSubculturing:Remove and discard culture medium. Briefly rinse the cell layer with DPBS solution to remove all traces of serum that contains trypsin inhibitor.Add 1.0 to 2.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 2 to 3 minutes). Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.Add 4.0 to 6.0 mL of complete growth medium and aspirate cells by gently pipetting. Add appropriate aliquots of the cell suspension to new culture vessels.Ratio:1:2-1:4Renewal:Every 2 to 3 daysCyropreservation:Freeze medium: 60% Basal medium+30% FBS+10% DMSOStorage temperature: Liquid nitrogen vapor phaseCulture Conditions:Atmosphere: Air, 95%; CO2, 5%Temperature: 37°C