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| 数量 | 3-4周 |
| 供应商 | 博士德生物 |
| 检测方法 | 酶联免疫竞争法 |
| 应用 | Serumandplasma |
| 适应物种 | Mouse |
| 规格 | 96T |
Mouse/Rat Testosterone ELISA is based on the principle of competitive binding between Testosterone in the test specimen and Testosterone-HRP conjugate for a constant amount of rabbit anti-Testosterone. In the incubation, goat anti-rabbit IgG-coated wells are incubated with 25ml of Testosterone standards, controls, patient samples, 100 ml Testosterone-HRP conjugate reagent and 50ml rabbit anti-Testosterone reagent at room temperature 60 minutes. During the incubation, a fixed amount of HRP-labeled Testosterone competes with the endogenous Testosterone in the standard, sample, or quality control serum for a fixed number of binding sites of the specific Testosterone antibody. Thus, the amount of Testosterone peroxidase conjugate immunologically bound to the well progressively decreases as the concentration of Testosterone in the specimen increases. Unbound Testosterone peroxidase conjugate is then removed and the wells washed. Next, a solution of TMB Reagent is then added and incubated at room temperature for 15 minutes, resulting in the development of blue color. The color development is stopped with the addition of stop solution, and the absorbance is measured spectrophotometrically at 450nm.
| Product Name | Mouse Rat Testosterone ELISA Kit |
|---|---|
| SKU/Catalog Number | EK7014 |
| Description | Competitive High Sensitivity ELISA kit for Quantitative Detection of Mouse/Rat Testosterone. 96wells/kit, with removable strips. |
| Cite This Product | Mouse Rat Testosterone ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK7014) |
| Validated Species | Mouse, Rat |
| Application | ELISA *Our Boster Guarantee covers the use of this product in the above tested applications. |
| Cross Reactivity | There is no detectable cross-reactivity. |
| Pack Size | 96wells/kit, with removable strips. |
| Sensitivity | 0.1 ng/ml *Sensitivity, or Lower Limit of Detection (LLD), is the minimum level of target protein the ELISA assay can detect. We measure 20 blank wells and if the O.D. value is 2 standard deviations higher than the blanks' average O.D. the sample can be deemed positive. |
|---|---|
| Assay Range | 0.1-18 ng/ml *This assay range is determined using common samples. For samples with low target protein concentrations, users can adjust the standard curve to extend the lower limit of assay range. |
| Sample Type | Serum and plasma *The above listed samples are the ones valided with the assay. If you do not see your sample of interest listed, as long as there is enough level of target protein present in the sample, this Picokine? ELISA kit should detect it. **For protocol and tips regarding preparing your sample of interest, please check our ELISA sample preparation guide. |
| Storage | Store the kit at 2°C to 8°C. Keep microwells sealed in a dry bag with desiccants. The reagents are stable until expiration of the kit. Do not expose reagent to heat, sun, or strong light. Avoid multiple freeze-thaw cycles(Shipped with wet ice.) |
| Description | Quantity |
| Microwells coated with Goat Anti-Rabbit IgG | 12x8x1 Microwells |
| Standards | 6 vials ( ready to use) 0.5 ml |
| Rabbit Anti-Testosterone Reagent | 1 vial 7ml |
| Assay Diluent | 1 bottle (ready to use) 12ml |
| Enzyme Conjugate Conc. (20X) | 1 Vial 0.7ml |
| TMB Substrate | 1 bottle (ready to use) 12ml |
| Stop Solution | 1 bottle (ready to use) 12ml |
| 20X Wash Buffer | 1 bottle 25ml |
1. Distilled or deionized water
2. Precision pipettes
3. Disposable pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
4. ELISA reader capable of reading absorbance at 450nm
5. Absorbance paper or paper towel
6. Graph paper
Testosterone is a steroid hormone that stimulates development of male secondary sexual characteristics, produced mainly in the testes, but also in the ovaries and adrenal cortex.
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