Innovative Research's Human Lactoferrin ELISA kit is designed for detection of human lactoferrin in human biological samples. This assay employs a quantitative sandwich enzyme immunoassay technique, which measures lactoferrin in less than 4 hours. A polyclonal antibody specific for lactoferrin has been precoated onto a 96-well microplate with removable strips. Lactoferrin in standards and samples is sandwiched by the immobilized antibody and a biotinylated polyclonal antibody specific for lactoferrin, which is recognized by a streptavidin-peroxidase conjugate. All unbound material is then washed away and a peroxidase enzyme substrate is added. The color development is stopped and the intensity of the color is measured.
Lactoferrin is an 82.4kDa 691 amino acid glycoprotein which carries 2 ferric ions per molecule and is the main binding protein in milk. Lactoferrin has multiple physiological functions including iron absorption regulation and antibacterial, antiviral, and antiparasitic activity. Degranulated neutrophils are the primary source of plasma lactoferrin, which is increased during inflammation and bacterial infection.
