本公司提供科研TPO甲状腺过氧化物酶抗体，抗体质量可靠，订购TPO甲状腺过氧化物酶抗体请联系在线客服或者销售人员。
抗体参数如下>>>>
中文名称：甲状腺过氧化物酶抗体
英文名称：Anti-TPO
货号：bs-0447R
抗体来源：兔
克隆类型：多克隆
蛋白分子量：predicted molecular weight: 103kDa
纯化方法：affinity purified by Protein A
交叉反应：hu, mo, rat
测试应用：ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500
（石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
产品背景介绍：TPO (thyroid peroxidase) is single-pass type I membrane-bound glycoprotein, iodinates tyrosine residues in thyroglobulin to generate biologically active thyroid hormones T3 and T4.and Iodination and coupling of the hormonogenic tyrosines in thyroglobulin to yield the thyroid hormones T(3) and T(4). Binds 1 calcium ion per heterodimer. Belongs to the peroxidase family. XPO subfamily.Function : Iodination and coupling of the hormonogenic tyrosines in thyroglobulin to yield the thyroid hormones T(3) and T(4).Subcellular Location : Membrane and Cell surface.Post-translational modifications : Glycosylated.Heme is covalently bound through a H(2)O(2)-dependent autocatalytic process. Heme insertion is important for the delivery of protein at the cell surface.DISEASE : Note=An alternative splicing in the thyroperoxidase mRNA can cause Graves' disease.Defects in TPO are the cause of congenital hypothyroidism due to dyshormonogenesis type 2A (CHDH2A) [MIM:274500]; also called genetic defect in thyroid hormonogenesis 2A or thyroid hormone organification defect II. CHDH2A is due to defective conversion of accumulated iodide to organically bound iodine. The iodide organification defect can be partial or complete.Similarity : Belongs to the peroxidase family. XPO subfamily.Contains 1 EGF-like domain.Contains 1 Sushi (CCP/SCR) domain.甲状腺过氧化物酶TPO是一种含铁卟啉的血红蛋白类物质，位于甲状腺上皮细胞的顶端细胞膜上。TPO主要的生物学作用是使碘活化，酪氨酸碘化和促进碘化酪氨酸的耦联，在甲状腺激素合成过程中起着关键作用-是甲状腺激素合成过程中的关键酶. 产品图片Tissue/cell: human thyroid gland; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-TPO Polyclonal Antibody, Unconjugated(bs-0447R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining