找化学品上960化工网！
| 规格 | 25 assays/100 assays/200 assays/400 assays |
Cysteine-dependent aspartate-directed proteases (Caspases) are a family of cysteine proteases that play important roles in apoptosis, necrosis, and inflammation. Sequential activation of caspases plays an important role in cell apoptosis. Caspase-6 is a member of the caspase family. Caspase-6 is processed by caspases 7, 8 and 10, and can also undergo self-processing. Caspase-6 acts as a downstream enzyme in the caspase activation cascade and interacts with Caspase 8.
Caspase-6 Fluorometric Assay Kit provides a convenient and simple way for detecting the VEID-dependent caspase activity. VEID-AFC (AFC: 7-amino-4-trifluoromethyl coumarin) emits blue light (λmax = 400 nm); while cleavage of the substrate VEID-AFC by Caspase-6 or related caspases, free AFC emits a yellow-green fluorescence (λmax = 505 nm), which can be quantified by using a fluorecence microtiter plate reader or a fluorometer. Comparison of the fluorescence of free AFC from an apoptotic sample with an uninduced control determines the fold increase in Caspase-6 activity.
| Features | Fast and convenient Simple one-step procedure; takes 1-2 hours. Comparison of the fluorescence of AFC from an apoptotic sample with an uninduced control allows determination of the fold increase in caspase-6 activity. |
| Shipping | Gel pack |
| Storage Conditions | Store at -20℃. |
| Species reactivity | Mammalian |
| Applications | Detect early/middle stages of apoptosis; differentiate apoptosis from necrosis. |
| Kit components | Cell Lysis Buffer 2 × Reaction Buffer VEID-AFC (1 mM) DTT (1 M) |
