细胞核因子/k基因结合核因子 p52/p100抗体价格价格,详情介绍-960化工网

细胞核因子/k基因结合核因子 p52/p100抗体价格

价格：¥1000 - 3500

品牌：上海博湖

产地：进口、国产

最新更新日期：2021-11-18 10:20

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公司：上海博湖生物科技有限公司

联系人：韩丽君

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产品属性

抗体名	细胞核因子/k基因结合核因子 p52/p100抗体价格

抗体英文名

Anti-NFKB p52

靶点

详见说明书

浓度

1mg/1ml

应用范围	WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500

宿主

详见说明书

供应商

上海博湖

数量

大量

级别

详见说明书

目录编号

详见说明书

抗原来源

Rabbit

保质期

详见说明书

适应物种

详见说明书

标记物

详见说明书

克隆性

多克隆

保存条件	Store at -20 °C

形态

详见说明书

亚型

IgG

免疫原	KLH conjugated synthetic peptide derived from human NFKB p52

规格	0.2ml/200μg

产品说明

英文名称 Anti-NFKB p52
中文名称细胞核因子/k基因结合核因子 p52/p100抗体价格
别名 NFkB p100; p52; DNA binding factor KBF2; H2TF1; Lymphocyte translocation chromosome 10; Lyt10; Oncogene Lyt 10; DNA binding factor KBF2; DNA-binding factor KBF2; H2TF1; Lymphocyte translocation chromosome 10; Lymphocyte translocation chromosome 10 protein; Lyt 10; Lyt10; NFKB2; NFKB2_HUMAN; Nuclear factor NF kappa B p100 subunit; Nuclear factor NF kappa B p52 subunit; Nuclear factor NF-kappa-B p52 subunit; Nuclear factor of kappa light polypeptide gene enhancer in B cells 2; Nuclear factor of kappa light polypeptide gene enhancer in B-cells 2; Oncogene Lyt 10; Oncogene Lyt-10; p49/p100.

细胞核因子/k基因结合核因子 p52/p100抗体价格浓度 1mg/1ml
规格 0.1ml/100μg 0.2ml/200μg
抗体来源 Rabbit
克隆类型 polyclonal
交叉反应 Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse
产品类型一抗
研究领域肿瘤细胞生物信号转导细胞凋亡转录调节因子激酶和磷酸酶表观遗传学
蛋白分子量 predicted molecular weight: 50, 100kDa
性状 Lyophilized or Liquid
免疫原 KLH conjugated synthetic peptide derived from human NFKB p52
亚型 IgG
纯化方法 affinity purified by Protein A
储存液 Preservative: 15mM Sodium Azide, Constituents: 1% BSA, 0.01M PBS, pH 7.4

细胞核因子/k基因结合核因子 p52/p100抗体价格产品应用 WB=1:100-500 ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500
（石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
保存条件 Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C.
Important Note This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
产品介绍 NFkB is formed through the association of multiple subunits, either as a homodimer or heterodimer. Subunits have been identified as p50 (NFkB1), p65 (RelA), c-Rel, RelB and p52 (NFkB2). The classic NFkB form exists as a p50-p65 heterodimer and predominates in many cell types. Many of the possible combinatorial forms of homo- and heterodimers have been identified and growing evidence indicates that different forms of NFkB have different functions in cells. Interestingly, both the p50 and p52 subunits are derived from the precursor proteins p105 and p100 respectively, that each contain multiple copies of the so called ankyrin repeat at their C termini. Nuclear translocation of NFkB is confirmed by the use of electrophorectic mobility shift assays or by immunoblotting with nuclear extracts. The subunit composition of NFkB is confirmed by the use of antibodies that "supershift" the DNA/protein complex.
Function : NF-kappa-B is a pleiotropic transcription factor present in almost all cell types and is the endpoint of a series of signal transduction events that are initiated by a vast array of stimuli related to many biological processes such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. NF-kappa-B is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with other cofactors or corepressors. NF-kappa-B complexes are held in the cytoplasm in an inactive state complexed with members of the NF-kappa-B inhibitor (I-kappa-B) family. In a conventional activation pathway, I-kappa-B is phosphorylated by I-kappa-B kinases (IKKs) in response to different activators, subsequently degraded thus liberating the active NF-kappa-B complex which translocates to the nucleus. In a non-canonical activation pathway, the MAP3K14-activated CHUK/IKKA homodimer phosphorylates NFKB2/p100 associated with RelB, inducing its proteolytic processing to NFKB2/p52 and the formation of NF-kappa-B RelB-p52 complexes. The NF-kappa-B heterodimeric RelB-p52 complex is a transcriptional activator. The NF-kappa-B p52-p52 homodimer is a transcriptional repressor. NFKB2 appears to have dual functions such as cytoplasmic retention of attached NF-kappa-B proteins by p100 and generation of p52 by a cotranslational processing. The proteasome-mediated process ensures the production of both p52 and p100 and preserves their independent function. p52 binds to the kappa-B consensus sequence 5'-GGRNNYYCC-3', located in the enhancer region of genes involved in immune response and acute phase reactions. p52 and p100 are respectively the minor and major form; the processing of p100 being relatively poor. Isoform p49 is a subunit of the NF-kappa-B protein complex, which stimulates the HIV enhancer in synergy with p65.Subunit : Component of the NF-kappa-B RelB-p52 complex. Homodimer; component of the NF-kappa-B p52-p52 complex. Component of the NF-kappa-B p65-p52 complex. Component of the NF-kappa-B p52-c-Rel complex. NFKB2/p52 interacts with NFKBIE. Component of a complex consisting of the NF-kappa-B p50-p50 homodimer and BCL3. Directly interacts with MEN1.Subcellular Location : Nucleus. Cytoplasm. Note=Nuclear, but also found in the cytoplasm in an inactive form complexed to an inhibitor (I-kappa-B).Post-translational modifications : While translation occurs, the particular unfolded structure after the GRR repeat promotes the generation of p52 making it an acceptable substrate for the proteasome. This process is known as cotranslational processing. The processed form is active and the unprocessed form acts as an inhibitor (I kappa B-like), being able to form cytosolic complexes with NF-kappa B, trapping it in the cytoplasm.Complete folding of the region downstream of the GRR repeat precludes processing.Subsequent to MAP3K14-dependent serine phosphorylation, p100 polyubiquitination occurs then triggering its proteasome-dependent processing. Constitutive processing is tightly suppressed by its C-terminal processing inhibitory domain, named PID, which contains the death domain.DISEASE : Note=A chromosomal aberration involving NFKB2 is found in a case of B-cell non Hodgkin lymphoma (B-NHL). Translocation t(10;14)(q24;q32) with IGHA1. The resulting oncogene is also called Lyt-10C alpha variant. Note=A chromosomal aberration involving NFKB2 is found in a cutaneous T-cell leukemia (C-TCL) cell line. This rearrangement produces the p80HT gene which encodes for a truncated 80 kDa protein (p80HT). Note=In B-cell leukemia (B-CLL) cell line, LB40 and EB308, can be found after heterogeneous chromosomal aberrations, such as internal deletions.Similarity : Contains 7 ANK repeats.Contains 1 death domain.Contains 1 RHD (Rel-like) domain.Database links : UniProtKB/Swiss-Prot: Q00653.4

细胞核因子/k基因结合核因子 p52/p100抗体价格抗体的鉴定：
1）抗体的效价鉴定：不管是用于诊断还是用于治疗,制备抗体的目的都是要求较高效价。不同的抗原制备的抗体,要求的效价不一。鉴定效价的方法很多,包括有试管凝集反应,琼脂扩散试验,酶联免疫吸附试验等。常用的抗原所制备的抗体一般都有约成的鉴定效价的方法,以资比较。如制备抗抗体的效价,一般就采用琼脂扩散试验来鉴定。
2）抗体的特异性鉴定：抗体的特异性是指与相应抗原或近似抗原物质的识别能力。抗体的特异性高,它的识别能力就强。衡量特异性通常以交叉反应率来表示。交叉反应率可用竞争抑制试验测定。以不同浓度抗原和近似抗原分别做竞争抑制曲线,计算各自的结合率,求出各自在IC50时的浓度,并按公式计算交叉反应率。
如果所用抗原浓度IC50浓度为pg/管,而一些近似抗原物质的IC50浓度几乎是无穷大时,表示这一抗血清与其他抗原物质的交叉反应率近似为0,即该血清的特异性较好。
3）亲和力：是指抗体和抗原结合的牢固程度。亲和力的高低是由抗原分子的大小,抗体分子的结合位点与抗原决定簇之间立体构型的合适度决定的。有助于维持抗原抗体复合物稳定的分子间力有氢键,疏水键,侧链相反电荷基因的库仑力,范德华力和空间斥力。亲和力常以亲和常数K表示,K的单位是L/mol。抗体亲和力的测定对抗体的筛选,确定抗体的用途,验证抗体的均一性等均有重要意义。
种属：
兔抗单克隆抗体，鼠抗单克隆抗体。兔抗多克隆抗体。抗体的浓度为1mg/ml。抗体及相关标记抗体：HRP标记抗体，Biotin标记抗体，Gold标记抗体，RBITC标记抗体，AP标记抗体,FITC标记抗体，Cy3标记抗体，Cy5标记抗体，Cy5.5标记抗体，Cy7标记抗体，PE标记抗体，PE-Cy3标记抗体，PE-Cy5标记抗体，PE-Cy5.5标记抗体，PE-Cy7标记抗体，APC标记抗体，AlexaFluor 350标记抗体，Alexa Fluor 488标记抗体，Alexa Fluor 555标记抗体，Alexa Fluor647标记抗体抗体的交叉反应：人，小鼠，大鼠，鸡，狗，猪，羊，牛，兔等...... 抗体的应用：可以用于做石蜡切片免疫组化，冰冻切片免疫组化，Elisa，WB，免疫荧光等实验。
细胞核因子/k基因结合核因子 p52/p100抗体价格实验原理 :
（1）特异性结合抗原：抗体本身不能直接溶解或杀伤带有特异抗原的靶细胞，通常需要补体或吞噬细胞等共同发挥效应以清除病原微生物或导致病理损伤。然而，抗体可通过与病毒或毒素的特异性结合，直接发挥中和病毒的作用。
（2）活补体：IgM、IgG1、IgG2和IgG3可通过经典途径激活补体，凝聚的IgA、IgG4和IgE可通过替代途径激活补体。
（3）结合细胞：不同类别的免疫球蛋白，可结合不同种的细胞，参与免疫应答。
（4）可通过胎盘及粘膜：免疫球蛋白G（IgG）能通过胎盘进入胎儿血流中，使胎儿形成自然被动
免疫。免疫球蛋白A（IgA）可通过消化道及呼吸道粘膜，是粘膜局部抗感染免疫的主要因素。
（5）具有抗原性：抗体分子是一种蛋白质，也具有刺机体产生免疫应答的性能。不同的免疫球蛋白分子，各具有不同的抗原性。
（6）抗体对理化因子的抵抗力与一般球蛋白相同：不耐热，60～70℃即被破坏。各种酶及能使蛋白质凝固变性的物质，均能破坏抗体的作用。抗体可被中性盐类沉淀。在生产上常可用硫酸铵或硫酸钠从免疫血清中沉淀出含有抗体的球蛋白，再经透析法将其纯化。

Immunoprecipitation/ IP Kit - Anti-BLyS / TNFSF13B Immunomagnetic Beads IP 5 mL
Immunoprecipitation/ IP Kit - Anti-BLyS / TNFSF13B Immunomagnetic Beads IP 5 mL
Immunoprecipitation/ IP Kit - Anti-ITGB1 / Integrin beta-1 / CD29 Immunomagnetic Beads IP
IL18BP / IL18BPa 抗體, 兔單抗 ELISA 50 µg
IL18BP / IL18BPa 抗體, 兔多抗 ELISA 400 µg
RBP4 抗體, 鼠單抗 ELISA IHC-P 50 µg
RBP4 / PRBP 抗體, 鼠單抗 ELISA 50 µg
RBP4 抗體 (PE), 鼠單抗 FCM 25 Test
AKR1B1 抗體, 兔多抗 ELISA 400 µg
FLRT2 抗體, 鼠單抗 ELISA 50 µg
AKR1B1 抗體, 兔多抗, 抗原親和純化 ELISA WB IP 50 µg
Clusterin / Apolipoprotein J / Apo-J 抗體, 兔單抗 FCM IF ICC/IF 50 µg
Clusterin / Apolipoprotein J / Apo-J / CLU 抗體, 兔單抗 ELISA 50 µg
用于鉴别奈瑟氏菌的利用小牛血清的能力。奈瑟-普通琼脂生化鉴定管 20支/盒
用于鉴别奈瑟氏菌的利用糖源的能力。奈瑟-乳糖生化鉴定管 20支/盒
用于奈瑟氏菌属细菌生化鉴定奈瑟氏菌属细菌生化鉴定盒 8种×10次/盒
用于鉴别奈瑟氏菌的利用盐的能力。奈瑟-盐还原生化鉴定管 20支/盒
用于鉴别奈瑟氏菌的利用糖源的能力。奈瑟-蔗糖生化鉴定管 20支/盒
微生物学接种体肉汤 250g
双岐杆菌生化用基础培养基 250g
双歧杆菌培养基 250g
TPY肉汤 250g
TPY液体培养基 250g
细胞核因子/k基因结合核因子 p52/p100抗体价格人纤维蛋白原γELISA试剂盒 3.12-200 U/L
ELISA Kit for Human Fibrinogen gamma chain 3.12-200 U/L
人FMS样酪氨酸激酶3(Flt3)ELISA试剂盒 0.156-10 ng/mL
ELISA Kit for Human Receptor-type tyrosine-protein kinase FLT3 0.156-10 ng/mL
人酸性成纤维细胞生长因子1(aFGF-1)ELISA试剂盒 78-5000 pg/ml