【Molecular Wt.】27 KDa

【Description】Binds peptides derived from antigens that access the endocytic route of antigen presenting cells (APC) and presents them on the cell surface for recognition by the CD4 T-cells. The peptide binding cleft accomodates peptides of 10-30 residues. The peptides presented by MHC class II molecules are generated mostly by degradation of proteins that access the endocytic route, where they are processed by lysosomal proteases and other hydrolases. Exogenous antigens that have been endocytosed by the APC are thus readily available for presentation via MHC II molecules, and for this reason this antigen presentation pathway is usually referred to as exogenous. As membrane proteins on their way to degradation in lysosomes as part of their normal turn-over are also contained in the endosomal/lysosomal compartments, exogenous antigens must compete with those derived from endogenous components. Autophagy is also a source of endogenous peptides, autophagosomes constitutively fuse with MHC class II loading compartments. In addition to APCs, other cells of the gastrointestinal tract, such as epithelial cells, express MHC class II molecules and CD74 and act as APCs, which is an unusual trait of the GI tract. To produce a MHC class II molecule that presents an antigen, three MHC class II molecules (heterodimers of an alpha and a beta chain) associate with a CD74 trimer in the ER to form an heterononamer. Soon after the entry of this complex into the endosomal/lysosomal system where antigen processing occurs, CD74 undergoes a sequential degradation by various proteases, including CTSS and CTSL, leaving a small fragment termed CLIP (class-II-associated invariant chain peptide). The removal of CLIP is facilitated by HLA-DM via direct binding to the alpha-beta-CLIP complex so that CLIP is released. HLA-DM stabilizes MHC class II molecules until primary high affinity antigenic peptides are bound. The MHC II molecule bound to a peptide is then transported to the cell membrane surface. In B cells, the interaction between HLA-DM and MHC class II molecules is regulated by HLA-DO. Primary dendritic cells (DCs) also to express HLA-DO. Lysosomal miroenvironment has been implicated in the regulation of antigen loading into MHC II molecules, increased acidification produces increased proteolysis and efficient peptide loading.

【Species Reactivity】Human;Mouse;Rat

【Tested applications】WB;IHC-P

【Antibody type】Rabbit polyclonal Antibody

【Immunogen】KLH conjugated synthetic peptide derived from human HLA class II histocompatibility antigen DM beta chain 111-200/263

【Positive control】

【Conjugate】Non-conjugated

【Alternative names】DMA
DMB
DRB
HLA class II histocompatibility antigen DM beta chain
HLA DMB
HLADM
HLADP1B
Major histocompatibility complex clas
HLA class II histocompatibility antigen, DM beta chain
MHC class II antigen DMB
Really interesting new gene 7 protein
HLA-DMB
RING7
DMB_HUMAN
MHC2.

【Form】Liquid

【Stroage】Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.

【Stroage Buffer】0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

【Isotype】IgG

【Purity】affinity purified by Protein A

【Subcellular location】Cell membrane. Endoplasmic reticulum membrane. Golgi apparatus > trans-Golgi network membrane. Endosome membrane. Lysosome membrane. The MHC class II complex transits through a number of intracellular compartments in the endocytic pathway until it reaches the cell membrane for antigen presentation.

【Background References】

【DatasheetURL】http://www.huabio.com/upload/ER1913-01.pdf

【Uniprot ID】SwissProt: P28068 Human

【Raised in】Rabbit

【Clone No.】