Human apolipoprotein E (Apo-E) ELISA Kit

标准曲线:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml OD1 OD2 Average
1000 0.088 0.090 0.089
500 0.135 0.142 0.139
250 0.227 0.237 0.232
125 0.324 0.341 0.333
62.5 0.583 0.598 0.591
31.25 0.847 0.864 0.856
15.62 1.228 1.235 1.232
0 2.155 2.199 2.177

Human apolipoprotein E (Apo-E) ELISA Kit

精密度:
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Human apolipoprotein E (Apo-E) ELISA Kit

线性度:
To assess the linearity of the assay, samples were spiked with high concentrations of human Apo-E in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
Sample Serum(n=4)
1:100 Average % 91
Range % 86-95
1:200 Average % 102
Range % 97-107
1:400 Average % 91
Range % 85-97
1:800 Average % 97
Range % 91-103

Human apolipoprotein E (Apo-E) ELISA Kit

回收率:
The recovery of human Apo-E spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 95 89-98
EDTA plasma (n=4) 97 90-100