Human IL-2 PicoKine ELISA Kit价格,详情介绍-960化工网

Human IL-2 PicoKine ELISA Kit

价格：￥1500 - 1980

品牌：BOSTER

产地：武汉

最新更新日期：2022-03-24 20:17

我要认领

公司：

联系人：客服部

联系电话： 027-67845390

联系邮箱：boster@boster.com.cn

产品属性

数量

现货

供应商

博士德生物

检测方法

酶联免疫双抗夹心法

应用	cell culture supernates, serum and plasma( heparin, EDTA, citrate).

适应物种

Human

规格

48T/96T

产品说明

货号	EK0397
产品名称	Human IL-2 PicoKine ELISA Kit
产品描述	For quantitative detection of human IL-2 in cell culture supernates, serum and plasma( heparin, EDTA, citrate).
免疫原	E.coli,A21-T153
交叉反应	There is no detectable cross-reactivity with other relevant proteins.
敏感性	<1pg/ml
检测范围	15.6pg/ml-1000pg/ml
基因名称	IL2
基因全名	interleukin 2
Uniprot ID	P60568
指标别名	Aldesleukin\|IL 2\|IL-2\|IL2\|IL2_HUMAN\|Interleukin 2\|Interleukin-2\|Interleukin2\|Involved in regulation of T cell clonal expansion\|Lymphokine\|T cell growth factor\|T-cell growth factor\|TCGF
背景资料	Interleukin-2(IL2), formerly referred to as T-cell growth factor, is a powerfully immunoregulatory lymphokine that is produced by lectin- or antigen-activated T cells. It is produced not only by mature T lymphocytes on stimulation but also constitutively by certain T-cell lymphoma cell lines. The lymphokine interleukin-2(IL-2) is responsible for autocrine cell cycle progression and regulation of immune responses. IL-2 expression in mature thymocytes and T cells has been found to be tightly controlled by monoallelic expression. IL-2 can act as a growth hormone for both B and T lymphocytes. The human gene for interleukin 2(IL2) is assigned to chromosome 4. Human IL-2 is a 15.5KDa glycoprotein, consisting of 153 amino acids in precursor form and 133 amino acids in mature form.
显色时间	15-20

SKU	名称	数量
Catalog number	Description	Quantity
EK0397-CAP	96-well plate precoated with anti- human IL-2 antibody	1
EK0397-ST	Lyophilized recombinant human IL-2 standard	10ng/tubex2
EK0397-DA	Biotinylated anti- human IL-2 antibody	130ul(dilution 1:100)
AR1103	Avidin-Biotin-Peroxidase Complex(ABC)	130ul(dilution 1:100)
AR1106-1	Sample diluent buffer	30ml
AR1106-2	Antibody diluent buffer	12ml
AR1106-3	ABC diluent buffer	12ml
AR1104	TMB color developing agent	10ml
AR1105	TMB stop solution	10ml
PLA-SEA	Adhesive cover	4

*小贴士：额外的试剂盒部件可以单独购买。如需购买请和试剂盒一起下单避免额外运费。

1.96孔读板仪器。
2.自动洗板机.
3.移液器和枪头。建议使用多通道移液器/排枪。
4.Eppendorf 管。
5.额外洗板缓冲液 (neutral PBS 或 TBS。试剂盒内提供足量。如需额外缓冲液可参考如下配方)。
- 0.01M TBS: Add 1.2g Tris, 8.5g Nacl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H₂O and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
- 0.01M PBS: Add 8.5g sodium chloride, 1.4g Na₂HPO₄ and 0.2g NaH₂PO₄ to 1000ml distilled water and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.

*样品准备的相关试剂也不含在试剂盒中。

(TMB 孵育条件： 37°C， 15-20)

Concentration(pg/ml)	0	15.6	31.2	62.5	125	250	500	1000
O.D.	0.005	0.049	0.106	0.199	0.371	0.734	1.471	2.213

*典型数据仅供参考。用户需要自己建立标准曲线。