Synonyms Fas ligand (TNF superfamily, member 6), APT1LG1, FASL, TNFSF6, CD178, tumor necrosis factor (ligand) superfamily member 6, Apoptosis antigen ligand, Fas antigen ligand, APTL, CD95-L.

Introduction The type II transmembrane protein FASLG is a member of the tumor necrosis factor (TNF) superfamily. A fas ligand/receptor interaction has a significant part in the regulation of the immune system and the advancement of cancer. FASLG is expressed on the activated T cell surface as a nondisulfidelinked homotrimer. FASLG binding to Fas/CD95/TNFRSF6 on a nearby cell prompts apoptosis in the Fas expressing cell. FASLG is released from the cell surface by metalloproteinases as a soluble molecule that stays trimeric and is able to bind with Fas, but its capability to activate apoptosis is radically reduced. In addition, FASLG binds to DcR3 - a soluble trap receptor with no signal transduction capabilities. Flawed Fas-mediated apoptosis causes oncogenesis in addition to drug resistance in existing tumors. Constitutive expression of FASLG in a variety of tumors enables their immune evasion. Both mouse and human FASLG are active on mouse and human cells.

Recombinant Human FAS LigandDescription Recombinant Human FAS Ligand produced in HEK293 cells is a non-glycosylated, polypeptide chain containing 147 amino acids (134-281a.a).

FASLG is fused to a 6 amino acid His-tag at N-terminus and purified by proprietary chromatographic techniques.

Source HEK293 cells.

Physical Appearance Sterile Filtered colorless solution.

Formulation The FASLG solution (0.6mg/ml) contains 1xPBS.

Stability FASLG Human Recombinant although stable at 4°C for 1 week, should be stored below -18°C.

Please prevent freeze thaw cycles.

Recombinant Human FAS LigandPurity Greater than 95.0% as determined by:

(a) Analysis by SEC-HPLC.

(b) Analysis by SDS-PAGE.

Biological Activity Fas ligand is biologically active as determined by its ability to induce cytotoxicity in Jurkat cells in the absence of any cross-linking. The expected ED50< 10 ng/ml, corresponding to a specific activity of 1x105 units/mg.

Recombinant Human FAS LigandUsage CHI''s products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.