上海中乔新舟生物科技有限公司是美国Sciencell公司在中国正规一级代理商：

美国ScienCell研究实验室（www.sciencellonline.com）成立于1999年，公司总部位于美国加州的圣地亚哥。主要致力于实验室
科研用原代细胞、原代细胞专用培养基、原代细胞无血清培养基、干细胞、干细胞培养基、干细胞无血清培养基的研究和开发，
在全球拥有众多客户。在国内销售15年来，很多老师应用其产品发表了高质量的SCI文章，且有着极高的文献引用率。凭借着严
格的质控和优秀的产品品质，深受广大科研工作者的信赖。

ScienCell研究实验室生产的原代细胞、原代细胞专用培养基都经过了严格的质量控制，细胞纯度可达98％。其中包括21种人体正
常细胞系统，90多种不同细胞类型。大多数细胞在全球唯有ScienCell实验室能够成功分离，产品质量过硬。

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人脑血管外膜成纤维细胞说明：

货号：1110

Description

Fibroblasts are mesenchymal cells which derived from the embryonic mesoderm. They have been extensively
used for a wide range of cellular and molecular studies. This is mainly because they are one of easiest types of
cells to grow in culture, and their durability makes them amenable to a wide variety of manipulations ranging
from studies employing gene transfection to microinjection. There is good evidence that fibroblasts in different
parts of the body are intrinsically different [1]. The vascular adventitia is defined as the outermost connective
tissue of vessels. One of the pathogenetic mechanisms involved is the functional derangements of vascular
fibroblasts. Their proliferative response contributes to the adventitial thickening observed during the development
of hypoxia-induced pulmonary hypertension [2].

HBVAF from ScienCell Research Laboratories are isolated from human brain vascular tissue. HBVAF are cryopreserved
at first passage of primary culture and delivered frozen. Each vial contains >5 x 10^5 cells in 1 ml volume. HBVAF
are characterized by spindle morphology and by immunofluorescent method with antibody to fibronectin. HBVAF
are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HBVAF are guaranteed to further expand
for 15 population doublings at the condition provided by ScienCell Research Laboratories.
Recommended Medium

It is recommended to use Fibroblast Medium (FM, Cat. No. 2301) for the culturing of HBVAF in vitro.
Product Use

HBVAF are for research use only. It is not approved for human or animal use, or for application in in vitro diagnostic
procedures.

Storage

Directly and immediately transfer cells from dry ice to liquid nitrogen upon receiving and keep the cells in liquid
nitrogen until cell culture needed for experiments.

Shipping

Dry ice.

Reference

[1] Conrad, G. W., Hart, G. W., Chen, Y. (1977) Differences in vitro between fibroblast-like cells from cornea, heart
and skin of embryonic chicks. J. Cell Sci. 26:119-137.
[2] Das M, Dempsey EC, Reeves JT, Stenmark KR. (2002) Selective expansion of fibroblast subpopulations from
pulmonary artery adventitia in response to hypoxia. Am J Physiol Lung Cell Mol Physiol 282(5):L976-86