| 供应商 | 上海联迈生物工程有限公司 |
| 数量 | 大量 |
| 目录编号 | LM-1508R |
| 克隆性 | 多克隆 |
| 抗原来源 | Rabbit |
| 保质期 | 1年 |
| 抗体英文名 | Cathepsin L |
| 抗体名 | 组织蛋白酶L抗体 |
| 宿主 | Rabbit |
| 适应物种 | Human, Mouse, Rat, |
| 免疫原 | KLH conjugated synthetic peptide derived from human cathepsin L1 proprotein:71-170/334 |
| 亚型 | IgG |
| 形态 | Lyophilized or Liquid |
| 应用范围 | WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:100-500 （石蜡切片需做抗原修复） |
| 浓度 | 1mg/ml |
| 保存条件 | Store at -20 °C |
| 规格 | 50ul 100ul 200ul |
| 英文名称 | Cathepsin L |
| 中文名称 | 组织蛋白酶L抗体 |
| 别 名 | Cathepsin L heavy chain; Cathepsin L light chain; CathepsinL; CATL; CTSL; CTSL protein; DKFZp686A18159; FLJ31037; Major excreted protein; MEP; cath-L; CL; CATL1_HUMAN; CathepsinL1; Cathepsin L1,Cathepsin-L1; CTSL 1; CTSL-1,CTS L1,CTS-L1. |
 | Specific References (1) | bs-1508R has been referenced in 1 publications. [IF=7.56] Hernáez B, Guerra M, Salas ML, Andrés G (2016) African Swine Fever Virus Undergoes Outer Envelope Disruption, Capsid Disassembly and Inner Envelope Fusion before Core Release from Multivesicular Endosomes. PLoS Pathog 12(4): e1005595. other ; Pig. PubMed:27110717 |
| 规格价格 | 50ul/780元 购买 100ul/1380元 购买 200ul/2200元 购买 大包装/询价 |
| 说 明 书 | 50ul 100ul 200ul |
| 研究领域 | 激酶和磷酸酶 合成与降解 |
| 抗体来源 | Rabbit |
| 克隆类型 | Polyclonal |
| 交叉反应 | Human, Mouse, Rat, |
| 产品应用 | WB=1:500-2000 ELISA=1:500-1000 IHC-P=1:400-800 IHC-F=1:400-800 IF=1:100-500 （石蜡切片需做抗原修复） not yet tested in other applications. optimal dilutions/concentrations should be determined by the end user. |
| 分 子 量 | 19/30/37kDa |
| 细胞定位 | 细胞浆 |
| 性 状 | Lyophilized or Liquid |
| 浓 度 | 1mg/ml |
| 免 疫 原 | KLH conjugated synthetic peptide derived from human cathepsin L1 proprotein:71-170/334 |
| 亚 型 | IgG |
| 纯化方法 | affinity purified by Protein A |
| 储 存 液 | 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol. |
| 保存条件 | Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20°C. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4 °C. |
| PubMed | PubMed |
| 产品介绍 | background: The protein encoded by this gene is a lysosomal cysteine proteinase that plays a major role in intracellular protein catabolism. Its substrates include collagen and elastin, as well as alpha-1 protease inhibitor, a major controlling element of neutrophil elastase activity. The encoded protein has been implicated in several pathologic processes, including myofibril necrosis in myopathies and in myocardial ischemia, and in the renal tubular response to proteinuria. This protein, which is a member of the peptidase C1 family, is a dimer composed of disulfide-linked heavy and light chains, both produced from a single protein precursor. At least two transcript variants encoding the same protein have been found for this gene. Function: Important for the overall degradation of proteins in lysosomes. Subunit: Dimer of a heavy and a light chain linked by disulfide bonds. Subcellular Location: Lysosome. Similarity: Belongs to the peptidase C1 family. SWISS: P06797 Gene ID: 1514 Database links: Entrez Gene: 1514 Human Entrez Gene: 13039 Mouse Entrez Gene: 25697 Rat Omim: 116880 Human SwissProt: P07711 Human SwissProt: P06797 Mouse SwissProt: P07154 Rat Unigene: 726015 Human Unigene: 930 Mouse Unigene: 1294 Rat Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications. 合成与降解（Synthesis and Degradation） 组织蛋白酶L(cathepsin L CL)属于木瓜蛋白酶家族中的半胱氨酸蛋白水解酶，以酶原的形式贮存于溶酶体中. 参与许多特殊的生理过程，如激素原的激活、抗原呈递、组织器官的发育等。 在病理状态下，因各种原因所致的细胞损伤(如病原微生物、炎症因子、氧化应激等)可降解细胞成分或细胞间质基质成分，包括层粘素、IV型胶原纤维及纤维连接素等，与人类许多疾病如肿瘤的浸润与转移、关节炎、骨质疏松、阿尔茨海姆病、多发性硬化症及其他慢性炎症性疾病有关. |
| 产品图片 |  Sample: D5-16 Cell (Human) Lysate at 30 ug D5-23 Cell (Human) Lysate at 40 ug Primary: Anti- Cathepsin L (bs-1508R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 19/30/37 kD Observed band size: 40/63 kD  Sample: A549 Cell (Human) Lysate at 30 ug K562 Cell (Human) Lysate at 30 ug Primary: Anti- Cathepsin L (bs-1508R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 19/30/37 kD Observed band size: 40/63 kD  Protein: line1, rat brain lysates, 30ug; line2, rat liver lysates, 30ug; Primary: Anti-Cathepsin L (bs-1508R) at 1:200; Secondary: HRP conjugated Goat-Anti-Rabbit IgG(bs-0295G-HRP) at 1: 3000; ECL excitated the fluorescence; Predicted band size : 37kD Observed band size : 19kD, 37kD  Tissue/cell: human cervical carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-Cathepsin Polyclonal Antibody, Unconjugated(bs-1508R) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining  Tissue/cell: rat pancreas tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-Cathepsin L Polyclonal Antibody, Unconjugated(bs-1508R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining |
