Pronase

General description

Pronase is a mixture of several nonspecific endo- and exoproteases that digest proteins down to single amino acids.

Other Notes

仅用于生命科学研究。不可用于诊断。

Preparation Note

Stabilizers: Protective effect of calcium ions

The preparation contains 20% calcium acetate for stability. It is free of starch as per the current Quality Control Procedures.The activity of a diluted solution containing 0.01–0.1 M calcium was stable over 24 hours at neutral pH at 4 to 8 °C. Pronase is also protected from heat inactivation by low levels of calcium.
Working concentration: 0.5 to 2 mg/ml
Working solution: Solvent is recommended in distilled water.
Stock solution is prepared by adding pronase powder to distilled water (10 to 20 mg/ml).
Storage conditions (working solution): -15 to -25 °C

Reconstitution

The lyophilizate is soluble in water (10 to 20 mg/ml).

Specificity

Pronase has a broad specificity, breaking down virtually all proteins into their individual amino acids; resolves carboxylic acids and alcohols.