谷丙转氨酶(ALT)重组蛋白适用生物 Rattus norvegicus (Rat，大鼠)
谷丙转氨酶(ALT)重组蛋白产品应用 SDS-PAGE; WB; ELISA; IP. 规格 50ug
分子量 28.5kDa 价格 请咨询当地代理商
纯度 > 95%
来源 原核表达
Recombinant Alanine Aminotransferase (ALT)
FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!
| Organism species | Rattus norvegicus (Rat) |
| Product No. | RPA207Ra02 |
| Source | Prokaryotic expression |
| Host | E.coli |
| Purity | > 95% |
| UOM | 50ug |
| Predicted Molecular Mass | 28.5kDa |
| Applications | SDS-PAGE; WB; ELISA; IP. |
| Endotoxin Level | <1.0EU per 1µg (determined by the LAL method) |
| Residues | Gln203~Leu443 (Accession # P25409) with N-terminal His-Tag |
| Formulation | Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl. |
SEQUENCES
The target protein is fused with N-terminal His-Tag, its sequence is listed below.
MGHHHHHHSGSEF-QVDYYLDE ERAWALDIAE LRRALCQARD RCCPRVLCVI NPGNPTGQVQ TRECIEAVIR FAFKEGLFLM ADEVYQDNVY AEGSQFHSFK KVLMEMGPPY STQQELASFH SVSKGYMGEC GFRGGYVEVV NMDAEVQKQM GKLMSVRLCP PVPGQALMDM VVSPPTPSEP SFKQFQAERQ EVLAELAAKA KLTEQVFNEA PGIRCNPVQG AMYSFPQVQL PLKAVQRAQE LGLAPDMFFC LCL
USAGE
Reconstitute in sterile PBS, pH7.2-pH7.4.
STORAGE AND STABILITY
谷丙转氨酶(ALT)重组蛋白Storage: Avoid repeated freeze/thaw cycles. Store at 2-8oC for one month. Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
About the MARKER (complimentary)
谷丙转氨酶(ALT)重组蛋白Effective Size Range: 10kDa to 70kDa.
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and 70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at double intensity to make location and size approximation of proteins of interest quick and easy.
Ready-to-use: No need to heat, dilute or add reducing agents before use.
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