白介素25(IL25)检测试剂盒(酶联免疫吸附试验法)
ELISA Kit for Interleukin 25 (IL25)
产品编号 SEB694Hu 适用生物 Homo sapiens (Human，人)
品牌商 Cloud-Clone Corp. (美国)
检测范围 15.63-1000pg/mL 灵敏度 5.4pg/mL
样本类型 Serum, plasma and other biological fluids.
实验方法 双抗夹心法
| 产品编号 | 中文名称 | | 英文名称 | 适用生物 |
 SEB694Hu | 白介素25(IL25)检测试剂盒(酶联免疫吸附试验法) | | ELISA Kit for Interleukin 25 (IL25) | Homo sapiens (Human) |
| SEB694Mu | 白介素25(IL25)检测试剂盒(酶联免疫吸附试验法) | | ELISA Kit for Interleukin 25 (IL25) | Mus musculus (Mouse) |
| SEB694Ra | 白介素25(IL25)检测试剂盒(酶联免疫吸附试验法) | | ELISA Kit for Interleukin 25 (IL25) | Rattus norvegicus (Rat) |
| SCB694Hu | 白介素25(IL25)检测试剂盒(化学发光免疫分析法) | | CLIA Kit for Interleukin 25 (IL25) | Homo sapiens (Human) |
| SCB694Mu | 白介素25(IL25)检测试剂盒(化学发光免疫分析法) | | CLIA Kit for Interleukin 25 (IL25) | Mus musculus (Mouse) |
| SCB694Ra | 白介素25(IL25)检测试剂盒(化学发光免疫分析法) | | CLIA Kit for Interleukin 25 (IL25) | Rattus norvegicus (Rat) |
ELISA Kit for Interleukin 25 (IL25)
FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!
| Organism species | Homo sapiens (Human) |
| Product No. | SEB694Hu |
| Sample type | Serum, plasma and other biological fluids. |
| Format | 96-well strip plate |
| Assay length | 4.5 hours |
| Detection range | 15.63-1000pg/mL The standard curve concentrations used for the ELISA’s were 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.25pg/mL, 15.63pg/mL |
| Sensitivity | The minimum detectable dose of this kit is typically less than 5.4pg/mL. |
Specificity
This assay has high sensitivity and excellent specificity for detection of Interleukin 25 (IL25).
No significant cross-reactivity or interference between Interleukin 25 (IL25) and analogues was observed.
Recovery
Matrices listed below were spiked with certain level of recombinant Interleukin 25 (IL25) and the recovery rates were calculated by comparing the measured value to the expected amount of Interleukin 25 (IL25) in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 98-105 | 102 |
| EDTA plasma(n=5) | 82-95 | 89 |
| heparin plasma(n=5) | 80-101 | 85 |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Interleukin 25 (IL25) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Interleukin 25 (IL25) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Interleukin 25 (IL25) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 90-97% | 79-103% | 87-94% | 85-97% |
| EDTA plasma(n=5) | 86-101% | 80-101% | 92-99% | 80-98% |
| heparin plasma(n=5) | 78-94% | 93-104% | 90-101% | 97-105% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
| Reagents | Quantity | Reagents | Quantity |
| Pre-coated, ready to use 96-well strip plate | 1 | Plate sealer for 96 wells | 4 |
| Standard | 2 | Standard Diluent | 1×20mL |
| Detection Reagent A | 1×120µL | Assay Diluent A | 1×12mL |
| Detection Reagent B | 1×120µL | Assay Diluent B | 1×12mL |
| TMB Substrate | 1×9mL | Stop Solution | 1×6mL |
| Wash Buffer (30 × concentrate) | 1×20mL | Instruction manual | 1 |
