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Recombinant Human S100B (N-6His) (CM19)

Recombinant Human S100B (N-6His) (CM19)Recombinant Human S100B (N-6His) (CM19)

产品说明(Description)

产品说明(产品说明(DescriptionDescription))

Recombinant Human S100 Calcium Binding Protein B is produced by our E.coli expression system and the target gene encoding Met1-Glu92 is expressed with a 6His tag at the N-terminus.\xa0

Recombinant Human S100 Calcium Binding Protein B is produced by our E.coli expression system and the target gene encoding Met1-Glu92 is expressed with a 6His tag at the N-terminus.\xa0

Accession #:\xa0P04271

Accession #:Accession #:\xa0P04271

Known as:\xa0Protein S100-B; S-100 protein beta chain; S-100 protein subunit beta; S100 calcium-binding protein B; S100b; S100 beta; S100 calcium binding protein B

Known as:\xa0Known as:\xa0Protein S100-B; S-100 protein beta chain; S-100 protein subunit beta; S100 calcium-binding protein B; S100b; S100 beta; S100 calcium binding protein B

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制剂(Formulation)

制剂(Formulation)制剂((Formulation))

Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.4.

Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.4.

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质量控制(Quality Control)\xa0

质量控制(Quality Control)\xa0质量控制((Quality Control))\xa0

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Purity:\xa0\xa0\xa0\xa0 \xa0 \xa0Greater than 95% as determined by reducing SDS-PAGE. \xa0 \xa0

Purity:\xa0\xa0\xa0Purity:\xa0\xa0\xa0\xa0 \xa0 \xa0Greater than 95% as determined by reducing SDS-PAGE. \xa0 \xa0

Endotoxin:\xa0Less than 0.1 ng/μg (1 EU/μg) as determined by LAL test.

Endotoxin:\xa0Endotoxin:\xa0Less than 0.1 ng/μg (1 EU/μg) as determined by LAL test.

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复溶(Reconstitution)

复溶(Reconstitution)复溶((Reconstitution))

Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100 μg/ml.
Dissolve the lyophilized protein in distilled water.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles. \xa0 \xa0

Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100 μg/ml.Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100 μg/ml.
Dissolve the lyophilized protein in distilled water.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles. \xa0 \xa0

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保存(Storage)

保存(Storage)保存((Storage))

Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.

Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.

Reconstituted protein solution can be stored at 4-7°C for 2-7 days.

Reconstituted protein solution can be stored at 4-7°C for 2-7 days.

Aliquots of reconstituted samples are stable at < -20°C for 3 months. \xa0\xa0 \xa0

Aliquots of reconstituted samples are stable at < -20°C for 3 months. \xa0\xa0 \xa0

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背景(Background)

背景(Background)背景((Background))

S100-B, is an acidic protein with a molecular weight of 21 kDa belonging to the S100 family. S100-B contains two EF-hand-type calcium-binding motifs separated by a hinge region with a hydrophobic cleft. S100-B plays an important role in neurodevelopment, differentiation, and brain construction. S100-B has neuroprotective effects, but at high concentrations S100-B is neurotoxic. Extracellular concentration of S100-B increases following brain damage, which easily penetrates into cerebrospinal fluid in brain damage and then into the blood. S100-B is expressed and produced by astrocytes in vertebrate brains and in the CNS, and the astrocytes are the major cells producing S100-B protein in gray matter, as well as oligodendrocytes are the predominant S100-B in protein producing cells in white matter. The major advantage of using S100-B is that elevations in serum or CSF levels provide a sensitive measure for determining CNS injury at the molecular level before gross changes develop, enabling timely delivery of crucial medical intervention before irreversible damage occurs. In addition, S100-B, which is also present in human melanocytes, is a reliable marker for melanoma malignancy both in bioptic tissue and in serum.

S100-B, is an acidic protein with a molecular weight of 21 kDa belonging to the S100 family. S100-B contains two EF-hand-type calcium-binding motifs separated by a hinge region with a hydrophobic cleft. S100-B plays an important role in neurodevelopment, differentiation, and brain construction. S100-B has neuroprotective effects, but at high concentrations S100-B is neurotoxic. Extracellular concentration of S100-B increases following brain damage, which easily penetrates into cerebrospinal fluid in brain damage and then into the blood. S100-B is expressed and produced by astrocytes in vertebrate brains and in the CNS, and the astrocytes are the major cells producing S100-B protein in gray matter, as well as oligodendrocytes are the predominant S100-B in protein producing cells in white matter. The major advantage of using S100-B is that elevations in serum or CSF levels provide a sensitive measure for determining CNS injury at the molecular level before gross changes develop, enabling timely delivery of crucial medical intervention before irreversible damage occurs. In addition, S100-B, which is also present in human melanocytes, is a reliable marker for melanoma malignancy both in bioptic tissue and in serum.

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电泳(SDS-PAGE)

电泳(SDS-PAGE)电泳((SDS-PAGE))

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FOR RESEARCH USE ONLY

FOR RESEARCH USE ONLYFOR RESEARCH USE ONLYFOR RESEARCH USE ONLY