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Recombinant Human Nectin-4 (C-6His) (CJ19)

Recombinant Human Nectin-4 (C-6His) (CJ19)Recombinant Human Nectin-4 (C-6His) (CJ19)

产品说明(Description)

产品说明(产品说明(DescriptionDescription))

Recombinant Human Poliovirus Receptor-Related Protein \xa04 is produced by our Mammalian expression system and the target gene encoding Gly32-Val351 is expressed with a 6His tag at the C-terminus.\xa0

Recombinant Human Poliovirus Receptor-Related Protein \xa04 is produced by our Mammalian expression system and the target gene encoding Gly32-Val351 is expressed with a 6His tag at the C-terminus.\xa0

Accession #:\xa0Q96NY8

Accession #:Accession #:\xa0Q96NY8

Known as:\xa0PVRL4; Nectin-4; Ig superfamily receptor LNIR; Poliovirus receptor-related protein 4; PRR4; LNIR; Nectin4

Known as:\xa0Known as:\xa0PVRL4; Nectin-4; Ig superfamily receptor LNIR; Poliovirus receptor-related protein 4; PRR4; LNIR; Nectin4

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制剂(Formulation)

制剂(Formulation制剂(Formulation))

Lyophilized from a 0.2 μm filtered solution of 20mM PB,150mM NaCl, pH7.4.

Lyophilized from a 0.2 μm filtered solution of 20mM PB,150mM NaCl, pH7.4.

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质量控制(Quality Control)\xa0

质量控质量控制(制(Quality ControlQuality Control)\xa0)\xa0

Purity: \xa0\xa0\xa0 \xa0 \xa0 Greater than 95% as determined by reducing SDS-PAGE. \xa0 \xa0

Purity: \xa0\xa0Purity: \xa0\xa0\xa0 \xa0 \xa0 Greater than 95% as determined by reducing SDS-PAGE. \xa0 \xa0

Endotoxin:\xa0\xa0Less than 0.1 ng/μg (1 EU/μg) as determined by LAL test.

Endotoxin:\xa0\xa0Endotoxin:\xa0\xa0Less than 0.1 ng/μg (1 EU/μg) as determined by LAL test.

Bioactivity:\xa0Measured by the ability of the immobilized protein to support the adhesion of NIH?3T3 mouse embryonic fibroblast cells.\xa0When 5 x 104 cells/well are added to recombinant human Nectin-4 coated plates (10 μg/mL with 100 μL/well), approximately >50% will adhere after 1 hour incubation at 37 °C.

Bioactivity:\xa0Bioactivity:\xa0Measured by the ability of the immobilized protein to support the adhesion of NIH?3T3 mouse embryonic fibroblast cells.\xa0When 5 x 104 cells/well are added to recombinant human Nectin-4 coated plates (10 μg/mL with 100 μL/well), approximately >50% will adhere after 1 hour incubation at 37 °C.

Bioactivity-Elisa:

Bioactivity-Elisa:Bioactivity-Bioactivity-Elisa:

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\t\t\t \t\t\t	\t\t\t 000000 \t\t\t	\t\t\t \t\t\t
\t\t\t Immobilized Anti-Human Nectin-4 mAb(Cat#NC017) at 2μg/ml (100 μl/well) can bind Human Nectin-4-His (Cat#CJ19).\xa0The ED50 of\xa0 Human Nectin-4-His (Cat#CJ19) is 42.5 ng/ml. \t\t\t \xa0 \t\t\t	\xa0	\t\t\t Immobilized Human Nectin-4-His (Cat#CJ19) at 5μg/ml (100 μl/well) can bind Human Nectin-1-His*(Cat#C492).*: Biotinylated by NHS-biotin prior to testing. The ED50 of Human Nectin-1-His*(Cat#C492) is 1.33 ug/ml. \t\t\t

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Immobilized Anti-Human Nectin-4 mAb(Cat#NC017) at 2μg/ml (100 μl/well) can bind Human Nectin-4-His (Cat#CJ19).\xa0The ED50 of\xa0 Human Nectin-4-His (Cat#CJ19) is 42.5 ng/ml.

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Immobilized Human Nectin-4-His (Cat#CJ19) at 5μg/ml (100 μl/well) can bind Human Nectin-1-His*(Cat#C492).*: Biotinylated by NHS-biotin prior to testing. The ED50 of Human Nectin-1-His*(Cat#C492) is 1.33 ug/ml.

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Immobilized Anti-Human Nectin-4 mAb(Cat#NC017) at 2μg/ml (100 μl/well) can bind Human Nectin-4-His (Cat#CJ19).\xa0The ED50 of\xa0 Human Nectin-4-His (Cat#CJ19) is 42.5 ng/ml.

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Immobilized Human Nectin-4-His (Cat#CJ19) at 5μg/ml (100 μl/well) can bind Human Nectin-1-His*(Cat#C492).*: Biotinylated by NHS-biotin prior to testing. The ED50 of Human Nectin-1-His*(Cat#C492) is 1.33 ug/ml.

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Immobilized Anti-Human Nectin-4 mAb(Cat#NC017) at 2μg/ml (100 μl/well) can bind Human Nectin-4-His (Cat#CJ19).\xa0The ED50 of\xa0 Human Nectin-4-His (Cat#CJ19) is 42.5 ng/ml.

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Immobilized Anti-Human Nectin-4 mAb(Cat#NC017) at 2μg/ml (100 μl/well) can bind Human Nectin-4-His (Cat#CJ19).\xa0The ED50 of\xa0 Human Nectin-4-His (Cat#CJ19) is 42.5 ng/ml.

Immobilized Anti-Human Nectin-4 mAb(Cat#NC017) at 2μg/ml (100 μl/well) can bind Human Nectin-4-His (Cat#CJ19).\xa0The ED50 of\xa0 Human Nectin-4-His (Cat#CJ19) is 42.5 ng/ml.\t\t\t

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Immobilized Human Nectin-4-His (Cat#CJ19) at 5μg/ml (100 μl/well) can bind Human Nectin-1-His*(Cat#C492).*: Biotinylated by NHS-biotin prior to testing. The ED50 of Human Nectin-1-His*(Cat#C492) is 1.33 ug/ml.

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Immobilized Human Nectin-4-His (Cat#CJ19) at 5μg/ml (100 μl/well) can bind Human Nectin-1-His*(Cat#C492).*: Biotinylated by NHS-biotin prior to testing. The ED50 of Human Nectin-1-His*(Cat#C492) is 1.33 ug/ml.

Immobilized Human Nectin-4-His (Cat#CJ19) at 5μg/ml (100 μl/well) can bind Human Nectin-1-His*(Cat#C492).*: Biotinylated by NHS-biotin prior to testing. The ED50 of Human Nectin-1-His*(Cat#C492) is 1.33 ug/ml.\t\t\t\t\t\t

Bioactivity-BLI:

Bioactivity-BLI:Bioactivity-BLI:Bioactivity-BLI:

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\t\t\t Loaded Anti-Human Nectin-4 mAb-mFc(Cat#NC017) on AMQ Biosensor, can bind Human Nectin-4-His(Cat#CJ19) with an affinity constant of 0.60 nM as determined in BLI assay. \t\t\t	\t\t\t \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \t\t\t	\t\t\t \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0\xa0 \t\t\t

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\t\t\t\t\t\t\xa0\t\t\t\xa0\t\t\t\t\t\t\t\t\t\t

Loaded Anti-Human Nectin-4 mAb-mFc(Cat#NC017) on AMQ Biosensor, can bind Human Nectin-4-His(Cat#CJ19) with an affinity constant of 0.60 nM as determined in BLI assay.

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\xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0\xa0

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Loaded Anti-Human Nectin-4 mAb-mFc(Cat#NC017) on AMQ Biosensor, can bind Human Nectin-4-His(Cat#CJ19) with an affinity constant of 0.60 nM as determined in BLI assay.

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\xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0\xa0

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Loaded Anti-Human Nectin-4 mAb-mFc(Cat#NC017) on AMQ Biosensor, can bind Human Nectin-4-His(Cat#CJ19) with an affinity constant of 0.60 nM as determined in BLI assay.

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Loaded Anti-Human Nectin-4 mAb-mFc(Cat#NC017) on AMQ Biosensor, can bind Human Nectin-4-His(Cat#CJ19) with an affinity constant of 0.60 nM as determined in BLI assay.

Loaded Anti-Human Nectin-4 mAb-mFc(Cat#NC017) on AMQ Biosensor, can bind Human Nectin-4-His(Cat#CJ19) with an affinity constant of 0.60 nM as determined in BLI assay.\t\t\t\t\t\t\t\t\t

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\xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0\xa0

\xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0 \xa0\xa0\t\t\t\t\t\t

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复溶(Reconstitution)

复溶(Reconstitution复溶(Reconstitution))

Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100 μg/ml.
Dissolve the lyophilized protein in distilled water.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles. \xa0 \xa0

Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100 μg/ml.Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100 μg/ml.
Dissolve the lyophilized protein in distilled water.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles. \xa0 \xa0

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保存(Storage)

保存(保存(StorageStorage))

Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.

Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.

Reconstituted protein solution can be stored at 4-7°C for 2-7 days.

Reconstituted protein solution can be stored at 4-7°C for 2-7 days.

Aliquots of reconstituted samples are stable at < -20°C for 3 months. \xa0\xa0 \xa0

Aliquots of reconstituted samples are stable at < -20°C for 3 months. \xa0\xa0 \xa0

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背景(Background)

背景(背景(BackgroundBackground))

Nectin-4 (PVRL4) is a type I transmembrane glycoprotein which belongs to the nectin family of Ig superfamily proteins. It contains two Ig-like C2-type domains and one Ig-like V-type domain. PVRL4 seems to be involved in cell adhesion through trans-homophilic and -heterophilic interactions, the latter including specifically interactions with nectin-1. It does not act as receptor for alpha-herpesvirus entry into cells. It is predominantly expressed in placenta, the embryo and breast carcinoma. But it is not detected in normal breast epithelium. The soluble form is produced by proteolytic cleavage at the cell surface (shedding), probably by ADAM17. Mutations in this gene are the cause of ectodermal dysplasia-syndactyly syndrome type 1, an autosomal recessive disorder.

Nectin-4 (PVRL4) is a type I transmembrane glycoprotein which belongs to the nectin family of Ig superfamily proteins. It contains two Ig-like C2-type domains and one Ig-like V-type domain. PVRL4 seems to be involved in cell adhesion through trans-homophilic and -heterophilic interactions, the latter including specifically interactions with nectin-1. It does not act as receptor for alpha-herpesvirus entry into cells. It is predominantly expressed in placenta, the embryo and breast carcinoma. But it is not detected in normal breast epithelium. The soluble form is produced by proteolytic cleavage at the cell surface (shedding), probably by ADAM17. Mutations in this gene are the cause of ectodermal dysplasia-syndactyly syndrome type 1, an autosomal recessive disorder.

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电泳(SDS-PAGE)

电泳(电泳(SDS-PAGESDS-PAGE))

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FOR RESEARCH USE ONLY

FOR RESEARCH USE ONLYFOR RESEARCH USE ONLYFOR RESEARCH USE ONLY