\n 本公司提供科研CYP24A1细胞色素P450 24A1抗体CYP24A1细胞色素P450 24A1抗体，抗体质量可靠，订购CYP24A1细胞色素P450 24A1抗体CYP24A1细胞色素P450 24A1抗体请联系在线客服或者销售人员。
抗体参数如下>>>>
中文名称：细胞色素P450 24A1抗体细胞色素P450 24A1抗体
英文名称：Anti-CYP24A1Anti-CYP24A1
货号：bs-3899R
抗体来源：兔
克隆类型：多克隆
蛋白分子量：predicted molecular weight: 53/57kDa
纯化方法：affinity purified by Protein A
交叉反应：hu, mo, rat, chk, dog, hrs, pig, Rb, cow
测试应用：ELISA=1:500-1000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500
（石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
产品背景介绍：CYP24A1 (cytochrome P450, family 24, subfamily A, polypeptide 1) is a member of the cytochrome P450 superfamily. It is a mitochondrial enzyme responsible for inactivating vitamin D3 metabolites through the C-24 oxidation pathway. In regulating the level of vitamin D3, CYP24A1 plays a role in calcium homeostasis and the vitamin D endocrine system. CYP24A1 also has a role in maintaining calcium homeostasis. It catalyzes the NADPH-dependent 24-hydroxylation of 25-hydroxyvitamin D(3) in the presence of adrenodoxin and NADPH-adrenodoxin reductase.Subcellular Location : Mitochondrion.Similarity : Belongs to the cytochrome P450 family.产品图片Tissue/cell: human cervical carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min; Incubation: Anti-CYP24A1 Polyclonal Antibody, Unconjugated(bs-3899R) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining