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产品概况

产品概况

货号	EK0391
产品名称	Mouse IL-1 alpha PicoKine ELISA Kit
产品描述	For quantitative detection of mouse IL-1 alpha in cell culture supernates and serum.
免疫原	E.coli,S115-S270
交叉反应	There is no detectable cross-reactivity with other relevant proteins.
敏感性	<1pg/ml
检测范围	4.7pg/ml-300pg/ml
基因名称	IL1A
基因全名	interleukin 1, alpha
Uniprot ID	P01582
指标别名	Hematopoietin 1|IL 1 alpha|Il 1a|IL1|IL1 ALPHA|IL1A|IL1F1|Ilia|Interleukin 1 alpha|Interleukin 1 alpha precursor|Interleukin1 alpha|OTTHUMP00000203692|OTTMUSP00000016430|Preinterleukin 1|Pro interleukin 1|Pro interleukin 1 alpha|Prointerleukin 1|RP23-160G19.8
背景资料	Interleukin-1 alpha(IL-1 alpha) and interleukin-1 beta(IL-1 beta) are two biochemically distinct, but distantly related, polypeptidic cytokines that play a key role in inflammation, immunologic reactions, and tissue repair. IL-1 alpha has been implicated in the pathogenesis of infectious, autoimmune and inflammatory diseases. Recently, it has been shown that IL-1 alpha is identical to hematopoietin 1, which is described as a hematopoietic growth factor acting on early progenitor cells in synergy with other hematopoietic growth factors.1 The human interleukin 1 alpha gene is assigned to chromosome 2. Genetic polymorphisms at interleukin(IL-1alpha and IL-1beta) have been recently suggested to be associated with severity of adult periodontitis. The murine IL-1 alpha and IL-1 beta genes encode structurally and evolutionarily related cytokines that exert a regulatory role in numerous physiological processes including hemopoiesis. The standard product used in this kit is recombinant mouse IL-1alpha with the molecular mass of 18kDa.
显色时间	15-20

货号EK0391产品名称Mouse IL-1 alpha PicoKine ELISA Kit产品描述For quantitative detection of mouse IL-1 alpha in cell culture supernates and serum.免疫原E.coli,S115-S270交叉反应There is no detectable cross-reactivity with other relevant proteins.敏感性<1pg/ml检测范围4.7pg/ml-300pg/ml基因名称IL1A基因全名interleukin 1, alphaUniprot IDP01582指标别名Hematopoietin 1|IL 1 alpha|Il 1a|IL1|IL1 ALPHA|IL1A|IL1F1|Ilia|Interleukin 1 alpha|Interleukin 1 alpha precursor|Interleukin1 alpha|OTTHUMP00000203692|OTTMUSP00000016430|Preinterleukin 1|Pro interleukin 1|Pro interleukin 1 alpha|Prointerleukin 1|RP23-160G19.8背景资料Interleukin-1 alpha(IL-1 alpha) and interleukin-1 beta(IL-1 beta) are two biochemically distinct, but distantly related, polypeptidic cytokines that play a key role in inflammation, immunologic reactions, and tissue repair. IL-1 alpha has been implicated in the pathogenesis of infectious, autoimmune and inflammatory diseases. Recently, it has been shown that IL-1 alpha is identical to hematopoietin 1, which is described as a hematopoietic growth factor acting on early progenitor cells in synergy with other hematopoietic growth factors.1 The human interleukin 1 alpha gene is assigned to chromosome 2. Genetic polymorphisms at interleukin(IL-1alpha and IL-1beta) have been recently suggested to be associated with severity of adult periodontitis. The murine IL-1 alpha and IL-1 beta genes encode structurally and evolutionarily related cytokines that exert a regulatory role in numerous physiological processes including hemopoiesis. The standard product used in this kit is recombinant mouse IL-1alpha with the molecular mass of 18kDa.显色时间15-20货号EK0391货号货号EK0391EK0391产品名称Mouse IL-1 alpha PicoKine ELISA Kit产品名称产品名称Mouse IL-1 alpha PicoKine ELISA KitMouse IL-1 alpha PicoKine ELISA Kit产品描述For quantitative detection of mouse IL-1 alpha in cell culture supernates and serum.产品描述产品描述For quantitative detection of mouse IL-1 alpha in cell culture supernates and serum.For quantitative detection of mouse IL-1 alpha in cell culture supernates and serum.免疫原E.coli,S115-S270免疫原免疫原E.coli,S115-S270E.coli,S115-S270交叉反应There is no detectable cross-reactivity with other relevant proteins.交叉反应交叉反应There is no detectable cross-reactivity with other relevant proteins.There is no detectable cross-reactivity with other relevant proteins.敏感性<1pg/ml敏感性敏感性<1pg/ml<1pg/ml检测范围4.7pg/ml-300pg/ml检测范围检测范围4.7pg/ml-300pg/ml4.7pg/ml-300pg/ml基因名称IL1A基因名称基因名称IL1AIL1A基因全名interleukin 1, alpha基因全名基因全名interleukin 1, alphainterleukin 1, alphaUniprot IDP01582Uniprot IDUniprot IDP01582P01582指标别名Hematopoietin 1|IL 1 alpha|Il 1a|IL1|IL1 ALPHA|IL1A|IL1F1|Ilia|Interleukin 1 alpha|Interleukin 1 alpha precursor|Interleukin1 alpha|OTTHUMP00000203692|OTTMUSP00000016430|Preinterleukin 1|Pro interleukin 1|Pro interleukin 1 alpha|Prointerleukin 1|RP23-160G19.8指标别名指标别名Hematopoietin 1|IL 1 alpha|Il 1a|IL1|IL1 ALPHA|IL1A|IL1F1|Ilia|Interleukin 1 alpha|Interleukin 1 alpha precursor|Interleukin1 alpha|OTTHUMP00000203692|OTTMUSP00000016430|Preinterleukin 1|Pro interleukin 1|Pro interleukin 1 alpha|Prointerleukin 1|RP23-160G19.8Hematopoietin 1|IL 1 alpha|Il 1a|IL1|IL1 ALPHA|IL1A|IL1F1|Ilia|Interleukin 1 alpha|Interleukin 1 alpha precursor|Interleukin1 alpha|OTTHUMP00000203692|OTTMUSP00000016430|Preinterleukin 1|Pro interleukin 1|Pro interleukin 1 alpha|Prointerleukin 1|RP23-160G19.8背景资料Interleukin-1 alpha(IL-1 alpha) and interleukin-1 beta(IL-1 beta) are two biochemically distinct, but distantly related, polypeptidic cytokines that play a key role in inflammation, immunologic reactions, and tissue repair. IL-1 alpha has been implicated in the pathogenesis of infectious, autoimmune and inflammatory diseases. Recently, it has been shown that IL-1 alpha is identical to hematopoietin 1, which is described as a hematopoietic growth factor acting on early progenitor cells in synergy with other hematopoietic growth factors.1 The human interleukin 1 alpha gene is assigned to chromosome 2. Genetic polymorphisms at interleukin(IL-1alpha and IL-1beta) have been recently suggested to be associated with severity of adult periodontitis. The murine IL-1 alpha and IL-1 beta genes encode structurally and evolutionarily related cytokines that exert a regulatory role in numerous physiological processes including hemopoiesis. The standard product used in this kit is recombinant mouse IL-1alpha with the molecular mass of 18kDa.背景资料背景资料Interleukin-1 alpha(IL-1 alpha) and interleukin-1 beta(IL-1 beta) are two biochemically distinct, but distantly related, polypeptidic cytokines that play a key role in inflammation, immunologic reactions, and tissue repair. IL-1 alpha has been implicated in the pathogenesis of infectious, autoimmune and inflammatory diseases. Recently, it has been shown that IL-1 alpha is identical to hematopoietin 1, which is described as a hematopoietic growth factor acting on early progenitor cells in synergy with other hematopoietic growth factors.1 The human interleukin 1 alpha gene is assigned to chromosome 2. Genetic polymorphisms at interleukin(IL-1alpha and IL-1beta) have been recently suggested to be associated with severity of adult periodontitis. The murine IL-1 alpha and IL-1 beta genes encode structurally and evolutionarily related cytokines that exert a regulatory role in numerous physiological processes including hemopoiesis. The standard product used in this kit is recombinant mouse IL-1alpha with the molecular mass of 18kDa.Interleukin-1 alpha(IL-1 alpha) and interleukin-1 beta(IL-1 beta) are two biochemically distinct, but distantly related, polypeptidic cytokines that play a key role in inflammation, immunologic reactions, and tissue repair. IL-1 alpha has been implicated in the pathogenesis of infectious, autoimmune and inflammatory diseases. Recently, it has been shown that IL-1 alpha is identical to hematopoietin 1, which is described as a hematopoietic growth factor acting on early progenitor cells in synergy with other hematopoietic growth factors.1 The human interleukin 1 alpha gene is assigned to chromosome 2. Genetic polymorphisms at interleukin(IL-1alpha and IL-1beta) have been recently suggested to be associated with severity of adult periodontitis. The murine IL-1 alpha and IL-1 beta genes encode structurally and evolutionarily related cytokines that exert a regulatory role in numerous physiological processes including hemopoiesis. The standard product used in this kit is recombinant mouse IL-1alpha with the molecular mass of 18kDa.显色时间15-20显色时间显色时间15-2015-20

试剂盒内容

试剂盒内容

SKU	名称	数量
Catalog number	Description	Quantity
EK0391-CAP	96-well plate precoated with anti- mouse IL-1 alpha antibody	1
EK0391-ST	Lyophilized recombinant mouse IL-1 alpha standard	10ng/tubex2
EK0391-DA	Biotinylated anti- mouse IL-1 alpha antibody	130ul(dilution 1:100)
AR1103	Avidin-Biotin-Peroxidase Complex(ABC)	130ul(dilution 1:100)
AR1106-1	Sample diluent buffer	30ml
AR1106-2	Antibody diluent buffer	12ml
AR1106-3	ABC diluent buffer	12ml
AR1104	TMB color developing agent	10ml
AR1105	TMB stop solution	10ml
PLA-SEA	Adhesive cover	4

SKU名称数量Catalog numberDescriptionQuantityEK0391-CAP96-well plate precoated with anti- mouse IL-1 alpha antibody1EK0391-STLyophilized recombinant mouse IL-1 alpha standard10ng/tubex2EK0391-DABiotinylated anti- mouse IL-1 alpha antibody130ul(dilution 1:100)AR1103Avidin-Biotin-Peroxidase Complex(ABC)130ul(dilution 1:100)AR1106-1Sample diluent buffer30mlAR1106-2Antibody diluent buffer12mlAR1106-3ABC diluent buffer12mlAR1104TMB color developing agent10mlAR1105TMB stop solution10mlPLA-SEAAdhesive cover4SKU名称数量SKUSKU名称名称数量数量Catalog numberDescriptionQuantityCatalog numberCatalog numberDescriptionDescriptionQuantityQuantityEK0391-CAP96-well plate precoated with anti- mouse IL-1 alpha antibody1EK0391-CAPEK0391-CAP96-well plate precoated with anti- mouse IL-1 alpha antibody96-well plate precoated with anti- mouse IL-1 alpha antibody11EK0391-STLyophilized recombinant mouse IL-1 alpha standard10ng/tubex2EK0391-STEK0391-STLyophilized recombinant mouse IL-1 alpha standardLyophilized recombinant mouse IL-1 alpha standard10ng/tubex210ng/tubex2EK0391-DABiotinylated anti- mouse IL-1 alpha antibody130ul(dilution 1:100)EK0391-DAEK0391-DABiotinylated anti- mouse IL-1 alpha antibodyBiotinylated anti- mouse IL-1 alpha antibody130ul(dilution 1:100)130ul(dilution 1:100)AR1103Avidin-Biotin-Peroxidase Complex(ABC)130ul(dilution 1:100)AR1103AR1103Avidin-Biotin-Peroxidase Complex(ABC)Avidin-Biotin-Peroxidase Complex(ABC)130ul(dilution 1:100)130ul(dilution 1:100)AR1106-1Sample diluent buffer30mlAR1106-1AR1106-1Sample diluent bufferSample diluent buffer30ml30mlAR1106-2Antibody diluent buffer12mlAR1106-2AR1106-2Antibody diluent bufferAntibody diluent buffer12ml12mlAR1106-3ABC diluent buffer12mlAR1106-3AR1106-3ABC diluent bufferABC diluent buffer12ml12mlAR1104TMB color developing agent10mlAR1104AR1104TMB color developing agentTMB color developing agent10ml10mlAR1105TMB stop solution10mlAR1105AR1105TMB stop solutionTMB stop solution10ml10mlPLA-SEAAdhesive cover4PLA-SEAPLA-SEAAdhesive coverAdhesive cover44*小贴士：额外的试剂盒部件可以单独购买。如需购买请和试剂盒一起下单避免额外运费。

需要用户自己提供的器材和试剂

需要用户自己提供的器材和试剂

• 1.96孔读板仪器。
• 2.自动洗板机.
• 3.移液器和枪头。建议使用多通道移液器/排枪。
• 4.Eppendorf 管。
• 5.额外洗板缓冲液 (neutral PBS 或 TBS。试剂盒内提供足量。如需额外缓冲液可参考如下配方)。
  • 0.01M TBS: Add 1.2g Tris, 8.5g Nacl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H₂O and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
  • 0.01M PBS: Add 8.5g sodium chloride, 1.4g Na₂HPO₄ and 0.2g NaH₂PO₄ to 1000ml distilled water and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.

1.96孔读板仪器。

1.96孔读板仪器。

2.自动洗板机.

2.自动洗板机.

3.移液器和枪头。建议使用多通道移液器/排枪。

3.移液器和枪头。建议使用多通道移液器/排枪。

4.Eppendorf 管。

4.Eppendorf 管。

5.额外洗板缓冲液 (neutral PBS 或 TBS。试剂盒内提供足量。如需额外缓冲液可参考如下配方)。

• 0.01M TBS: Add 1.2g Tris, 8.5g Nacl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H₂O and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
• 0.01M PBS: Add 8.5g sodium chloride, 1.4g Na₂HPO₄ and 0.2g NaH₂PO₄ to 1000ml distilled water and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.

5.额外洗板缓冲液 (neutral PBS 或 TBS。试剂盒内提供足量。如需额外缓冲液可参考如下配方)。

• 0.01M TBS: Add 1.2g Tris, 8.5g Nacl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H₂O and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
• 0.01M PBS: Add 8.5g sodium chloride, 1.4g Na₂HPO₄ and 0.2g NaH₂PO₄ to 1000ml distilled water and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.

0.01M TBS: Add 1.2g Tris, 8.5g Nacl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H₂O and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.

0.01M TBS: Add 1.2g Tris, 8.5g Nacl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H₂2O and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.

0.01M PBS: Add 8.5g sodium chloride, 1.4g Na₂HPO₄ and 0.2g NaH₂PO₄ to 1000ml distilled water and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.

0.01M PBS: Add 8.5g sodium chloride, 1.4g Na₂2HPO₄4 and 0.2g NaH₂2PO₄4 to 1000ml distilled water and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.*样品准备的相关试剂也不含在试剂盒中。

批内差/批间差

批内差/批间差

Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean(pg/ml)	15	63	151	16	60	155
Standard deviation	1	2.7	6.64	1.18	3.3	7.28
CV(%)	6.7%	4.3%	4.4%	7.4%	5.5%	4.7%

Intra-Assay PrecisionInter-Assay PrecisionSample123123n161616242424Mean(pg/ml)15631511660155Standard deviation12.76.641.183.37.28CV(%)6.7%4.3%4.4%7.4%5.5%4.7%Intra-Assay PrecisionInter-Assay PrecisionIntra-Assay PrecisionIntra-Assay PrecisionInter-Assay PrecisionInter-Assay PrecisionSample123123SampleSample112233112233n161616242424nn161616161616242424242424Mean(pg/ml)15631511660155Mean(pg/ml)Mean(pg/ml)1515636315115116166060155155Standard deviation12.76.641.183.37.28Standard deviationStandard deviation112.72.76.646.641.181.183.33.37.287.28CV(%)6.7%4.3%4.4%7.4%5.5%4.7%CV(%)CV(%)6.7%6.7%4.3%4.3%4.4%4.4%7.4%7.4%5.5%5.5%4.7%4.7%

Mouse IL-1 alpha PicoKine ELISA Kit的典型数据示例（取自最近的一批次质检数据）

Mouse IL-1 alpha PicoKine ELISA Kit的典型数据示例（取自最近的一批次质检数据）Mouse IL-1 alpha PicoKine ELISA Kit的典型数据示例（取自最近的一批次质检数据）(TMB 孵育条件： 37°C， 15-20)

Concentration(pg/ml)	0	4.7	9.4	18.8	37.5	75	150	300
O.D.	0.022	0.085	0.155	0.282	0.519	1.038	1.684	2.466

Concentration(pg/ml)04.79.418.837.575150300O.D.0.0220.0850.1550.2820.5191.0381.6842.466Concentration(pg/ml)04.79.418.837.575150300Concentration(pg/ml)Concentration(pg/ml)004.74.79.49.418.818.837.537.57575150150300300O.D.0.0220.0850.1550.2820.5191.0381.6842.466O.D.O.D.0.0220.0220.0850.0850.1550.1550.2820.2820.5190.5191.0381.0381.6841.6842.4662.466*典型数据仅供参考。用户需要自己建立标准曲线。