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产品描述：MIF (migration inhibitory factor) is a pro-inflammatory cytokine involved in the innate immune response to bacterial pathogens. The expression of MIF at sites of inflammation suggests a role as a mediator in regulating the functions of macrophages in host defense, and data has shown that MIF counteracts the anti-inflammatory activity of glucocorticoids. This cytokine has phenylpyruvate tautomerase and dopachrome tautomerase activity in vitro, but the physiological substrate is unknown. Human MIF acts on fibroblasts by inducing IL-1, IL-8 and MMP expression, and also stimulates NO production and TNF-alpha release following IFN-gamma activation on macrophages. Recombinant Human MIF forms a trimer in solution due to the interaction of the beta strands of the MIF proteins. Human MIF is a non-glycosylated polypeptide consisting of 115 amino acids with a MW of 12.5 kDa.
形 态 ：Each vial contains 25 ug protein lyophilized in 10 mM sodium phosphate pH7.5. Reconstitute with 0.25 ml sterile deionized water for a final concentration of 0.1 mg/ml.
来 源 ：E. coli. Endotoxin level as measured by LAL is <0.01ng/ug or <0.1EU/ug.
别 名 ：Macrophage migration inhibitory factor; MIF; phenylpyruvate tautomerase; L-dopachrome tautomerase; L-dopachrome isomerase; glycosylation-inhibiting factor; GIF; MIF; GLIF; MMIF
纯 度 ：Purity Approximately 97% as determined by RP-HPLC and reducing and non-reducing SDS-PAGE. Protein Content determined by UV spectroscopy at 280 nm and quantitation on SDS-PAGE against a known standard.
分子量 ：12500 Da
应 用 ：E, WB
Human MIF biological activity was measured in an agarose microdroplet assay using human U937 cells as targets. The optimal range of activity found for human MIF in this assay was 0.5-1ug/ml.
保存条件 ：Store at -20°C. Product is guaranteed one year from the date of shipment. Following reconstitution, store at -20°C. We recommend to add a carrier protein (0.1% HSA or BSA) for long term storage.
产品链接 ：http://www.abbiotec.com/proteins/human-mif 产品描述：产品描述：
MIF (migration inhibitory factor) is a pro-inflammatory cytokine involved in the innate immune response to bacterial pathogens. The expression of MIF at sites of inflammation suggests a role as a mediator in regulating the functions of macrophages in host defense, and data has shown that MIF counteracts the anti-inflammatory activity of glucocorticoids. This cytokine has phenylpyruvate tautomerase and dopachrome tautomerase activity in vitro, but the physiological substrate is unknown. Human MIF acts on fibroblasts by inducing IL-1, IL-8 and MMP expression, and also stimulates NO production and TNF-alpha release following IFN-gamma activation on macrophages. Recombinant Human MIF forms a trimer in solution due to the interaction of the beta strands of the MIF proteins. Human MIF is a non-glycosylated polypeptide consisting of 115 amino acids with a MW of 12.5 kDa.
形 态 ：形 态 ：
Each vial contains 25 ug protein lyophilized in 10 mM sodium phosphate pH7.5. Reconstitute with 0.25 ml sterile deionized water for a final concentration of 0.1 mg/ml.
来 源 ：来 源 ：
E. coli. Endotoxin level as measured by LAL is <0.01ng/ug or <0.1EU/ug.
别 名 ：别 名 ：
Macrophage migration inhibitory factor; MIF; phenylpyruvate tautomerase; L-dopachrome tautomerase; L-dopachrome isomerase; glycosylation-inhibiting factor; GIF; MIF; GLIF; MMIF
纯 度 ：纯 度 ：
Purity Approximately 97% as determined by RP-HPLC and reducing and non-reducing SDS-PAGE. Protein Content determined by UV spectroscopy at 280 nm and quantitation on SDS-PAGE against a known standard.
分子量 ：分子量 ：
12500 Da
应 用 ：应 用 ：E, WB
Human MIF biological activity was measured in an agarose microdroplet assay using human U937 cells as targets. The optimal range of activity found for human MIF in this assay was 0.5-1ug/ml.
保存条件 ：保存条件 ：
Store at -20°C. Product is guaranteed one year from the date of shipment. Following reconstitution, store at -20°C. We recommend to add a carrier protein (0.1% HSA or BSA) for long term storage.
产品链接 ：产品链接 ：
http://www.abbiotec.com/proteins/human-mifhttp://www.abbiotec.com/proteins/human-mif
