\n 适用生物\xa0\xa0 \xa0Homo sapiens (Human，人)
肿瘤坏死因子相关凋亡诱导配体(TRAIL)检测试剂盒肿瘤坏死因子相关凋亡诱导配体(TRAIL)检测试剂盒
检测范围\xa0\xa0 \xa00.156-10ng/mL\xa0\xa0 \xa0灵敏度\xa0\xa0 \xa00.063ng/mL
样本类型\xa0\xa0 \xa0Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
实验时长\xa0\xa0 \xa04.5h\xa0\xa0 \xa0实验方法\xa0\xa0 \xa0双抗夹心法 \xa0\xa0
规格\xa0\xa0 \xa096T
肿瘤坏死因子相关凋亡诱导配体(TRAIL)检测试剂盒肿瘤坏死因子相关凋亡诱导配体(TRAIL)检测试剂盒

ELISA Kit for Tumor Necrosis Factor Related Apoptosis Inducing Ligand (TRAIL)FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t

Organism species	Homo sapiens (Human)
Product No.	SEA139Hu
Sample type	Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Format	96T
Assay length	4.5 hours
Detection range	0.156-10ng/mL The standard curve concentrations used for the ELISA’s were 10ng/mL, 5ng/mL, 2.5ng/mL, 1.25ng/mL, 0.625ng/mL, 0.313ng/mL, 0.156ng/mL
Sensitivity	The minimum detectable dose of this kit is typically less than 0.063ng/mL.

\t\t\t\t\t\tOrganism species\t\t\tHomo sapiens (Human)\t\t\t\t\t\t\tProduct No.\t\t\tSEA139Hu\t\t\t\t\t\t\tSample type\t\t\tSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.\t\t\t\t\t\t\tFormat\t\t\t96T\t\t\t\t\t\t\tAssay length\t\t\t4.5 hours\t\t\t\t\t\t\tDetection range\t\t\t0.156-10ng/mL The standard curve concentrations used for the ELISA’s were 10ng/mL, 5ng/mL, 2.5ng/mL, 1.25ng/mL, 0.625ng/mL, 0.313ng/mL, 0.156ng/mL\t\t\t\t\t\t\tSensitivity\t\t\tThe minimum detectable dose of this kit is typically less than 0.063ng/mL.\t\t\t\t\t\t\t\tOrganism species\t\t\tHomo sapiens (Human)\t\t\t\t\tOrganism speciesOrganism speciesOrganism species\t\t\tHomo sapiens (Human)Homo sapiens (Human)\t\t\t\t\t\t\tProduct No.\t\t\tSEA139Hu\t\t\t\t\tProduct No.Product No.Product No.\t\t\tSEA139HuSEA139Hu\t\t\t\t\t\t\tSample type\t\t\tSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.\t\t\t\t\tSample typeSample typeSample type\t\t\tSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.\t\t\t\t\t\t\tFormat\t\t\t96T\t\t\t\t\tFormatFormatFormat\t\t\t96T96T\t\t\t\t\t\t\tAssay length\t\t\t4.5 hours\t\t\t\t\tAssay lengthAssay lengthAssay length\t\t\t4.5 hours4.5 hours\t\t\t\t\t\t\tDetection range\t\t\t0.156-10ng/mL The standard curve concentrations used for the ELISA’s were 10ng/mL, 5ng/mL, 2.5ng/mL, 1.25ng/mL, 0.625ng/mL, 0.313ng/mL, 0.156ng/mL\t\t\t\t\tDetection rangeDetection rangeDetection range\t\t\t0.156-10ng/mL The standard curve concentrations used for the ELISA’s were 10ng/mL, 5ng/mL, 2.5ng/mL, 1.25ng/mL, 0.625ng/mL, 0.313ng/mL, 0.156ng/mL0.156-10ng/mL The standard curve concentrations used for the ELISA’s were 10ng/mL, 5ng/mL, 2.5ng/mL, 1.25ng/mL, 0.625ng/mL, 0.313ng/mL, 0.156ng/mL\t\t\t\t\t\t\tSensitivity\t\t\tThe minimum detectable dose of this kit is typically less than 0.063ng/mL.\t\t\t\t\tSensitivitySensitivitySensitivity\t\t\tThe minimum detectable dose of this kit is typically less than 0.063ng/mL.The minimum detectable dose of this kit is typically less than 0.063ng/mL.\t\t\tSpecificityThis assay has high sensitivity and excellent specificity for detection of Tumor Necrosis Factor Related Apoptosis Inducing Ligand (TRAIL).\xa0
No significant cross-reactivity or interference between Tumor Necrosis Factor Related Apoptosis Inducing Ligand (TRAIL) and analogues was observed.Recovery肿瘤坏死因子相关凋亡诱导配体(TRAIL)检测试剂盒肿瘤坏死因子相关凋亡诱导配体(TRAIL)检测试剂盒Matrices listed below were spiked with certain level of recombinant Tumor Necrosis Factor Related Apoptosis Inducing Ligand (TRAIL) and the recovery rates were calculated by comparing the measured value to the expected amount of Tumor Necrosis Factor Related Apoptosis Inducing Ligand (TRAIL) in samples.\xa0\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t

Matrix	Recovery range (%)	Average(%)
serum(n=5)	79-89	83
EDTA plasma(n=5)	89-103	97
heparin plasma(n=5)	88-96	92

\t\t\t\t\t\tMatrix\t\t\tRecovery range (%)\t\t\tAverage(%)\t\t\t\t\t\t\tserum(n=5)\t\t\t79-89\t\t\t83\t\t\t\t\t\t\tEDTA plasma(n=5)\t\t\t89-103\t\t\t97\t\t\t\t\t\t\theparin plasma(n=5)\t\t\t88-96\t\t\t92\t\t\t\t\t\t\t\tMatrix\t\t\tRecovery range (%)\t\t\tAverage(%)\t\t\t\t\tMatrixMatrix\t\t\tRecovery range (%)Recovery range (%)\t\t\tAverage(%)Average(%)\t\t\t\t\t\t\tserum(n=5)\t\t\t79-89\t\t\t83\t\t\t\t\tserum(n=5)serum(n=5)\t\t\t79-8979-89\t\t\t8383\t\t\t\t\t\t\tEDTA plasma(n=5)\t\t\t89-103\t\t\t97\t\t\t\t\tEDTA plasma(n=5)EDTA plasma(n=5)\t\t\t89-10389-103\t\t\t9797\t\t\t\t\t\t\theparin plasma(n=5)\t\t\t88-96\t\t\t92\t\t\t\t\theparin plasma(n=5)heparin plasma(n=5)\t\t\t88-9688-96\t\t\t9292\t\t\tPrecisionIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Tumor Necrosis Factor Related Apoptosis Inducing Ligand (TRAIL) were tested 20 times on one plate, respectively.\xa0
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Tumor Necrosis Factor Related Apoptosis Inducing Ligand (TRAIL) were tested on 3 different plates, 8 replicates in each plate.\xa0
CV(%) = SD/meanX100\xa0
Intra-Assay: CV<10%\xa0
Inter-Assay: CV<12%\xa0LinearityThe linearity of the kit was assayed by testing samples spiked with appropriate concentration of Tumor Necrosis Factor Related Apoptosis Inducing Ligand (TRAIL) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.\xa0\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t

Sample	1:2	1:4	1:8	1:16
serum(n=5)	81-91%	79-98%	96-104%	82-91%
EDTA plasma(n=5)	95-103%	93-105%	88-101%	95-105%
heparin plasma(n=5)	81-104%	98-105%	85-99%	93-102%

\t\t\t\t\t\tSample\t\t\t1:2\t\t\t1:4\t\t\t1:8\t\t\t1:16\t\t\t\t\t\t\tserum(n=5)\t\t\t81-91%\t\t\t79-98%\t\t\t96-104%\t\t\t82-91%\t\t\t\t\t\t\tEDTA plasma(n=5)\t\t\t95-103%\t\t\t93-105%\t\t\t88-101%\t\t\t95-105%\t\t\t\t\t\t\theparin plasma(n=5)\t\t\t81-104%\t\t\t98-105%\t\t\t85-99%\t\t\t93-102%\t\t\t\t\t\t\t\tSample\t\t\t1:2\t\t\t1:4\t\t\t1:8\t\t\t1:16\t\t\t\t\tSampleSample\t\t\t1:21:2\t\t\t1:41:4\t\t\t1:81:8\t\t\t1:161:16\t\t\t\t\t\t\tserum(n=5)\t\t\t81-91%\t\t\t79-98%\t\t\t96-104%\t\t\t82-91%\t\t\t\t\tserum(n=5)serum(n=5)\t\t\t81-91%81-91%\t\t\t79-98%79-98%\t\t\t96-104%96-104%\t\t\t82-91%82-91%\t\t\t\t\t\t\tEDTA plasma(n=5)\t\t\t95-103%\t\t\t93-105%\t\t\t88-101%\t\t\t95-105%\t\t\t\t\tEDTA plasma(n=5)EDTA plasma(n=5)\t\t\t95-103%95-103%\t\t\t93-105%93-105%\t\t\t88-101%88-101%\t\t\t95-105%95-105%\t\t\t\t\t\t\theparin plasma(n=5)\t\t\t81-104%\t\t\t98-105%\t\t\t85-99%\t\t\t93-102%\t\t\t\t\theparin plasma(n=5)heparin plasma(n=5)\t\t\t81-104%81-104%\t\t\t98-105%98-105%\t\t\t85-99%85-99%\t\t\t93-102%93-102%\t\t\tStabilityThe stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.\xa0
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.Reagents and materials provided\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t\t

Reagents	Quantity	Reagents	Quantity
Pre-coated, ready to use 96-well strip plate	1	Plate sealer for 96 wells	4
Standard	2	Standard Diluent	1×20mL
Detection Reagent A	1×120μL	Assay Diluent A	1×12mL
Detection Reagent B	1×120μL	Assay Diluent B	1×12mL
TMB Substrate	1×9mL	Stop Solution	1×6mL
Wash Buffer (30 × concentrate)	1×20mL	Instruction manual	1

\t\t\t\t\t\tReagents\t\t\tQuantity\t\t\tReagents\t\t\tQuantity\t\t\t\t\t\t\tPre-coated, ready to use 96-well strip plate\t\t\t1\t\t\tPlate sealer for 96 wells\t\t\t4\t\t\t\t\t\t\tStandard\t\t\t2\t\t\tStandard Diluent\t\t\t1×20mL\t\t\t\t\t\t\tDetection Reagent A\t\t\t1×120μL\t\t\tAssay Diluent A\t\t\t1×12mL\t\t\t\t\t\t\tDetection Reagent B\t\t\t1×120μL\t\t\tAssay Diluent B\t\t\t1×12mL\t\t\t\t\t\t\tTMB Substrate\t\t\t1×9mL\t\t\tStop Solution\t\t\t1×6mL\t\t\t\t\t\t\tWash Buffer (30 × concentrate)\t\t\t1×20mL\t\t\tInstruction manual\t\t\t1\t\t\t\t\t\t\t\tReagents\t\t\tQuantity\t\t\tReagents\t\t\tQuantity\t\t\t\t\tReagentsReagents\t\t\tQuantityQuantity\t\t\tReagentsReagents\t\t\tQuantityQuantity\t\t\t\t\t\t\tPre-coated, ready to use 96-well strip plate\t\t\t1\t\t\tPlate sealer for 96 wells\t\t\t4\t\t\t\t\tPre-coated, ready to use 96-well strip platePre-coated, ready to use 96-well strip plate\t\t\t11\t\t\tPlate sealer for 96 wellsPlate sealer for 96 wells\t\t\t44\t\t\t\t\t\t\tStandard\t\t\t2\t\t\tStandard Diluent\t\t\t1×20mL\t\t\t\t\tStandardStandard\t\t\t22\t\t\tStandard DiluentStandard Diluent\t\t\t1×20mL1×20mL\t\t\t\t\t\t\tDetection Reagent A\t\t\t1×120μL\t\t\tAssay Diluent A\t\t\t1×12mL\t\t\t\t\tDetection Reagent ADetection Reagent A\t\t\t1×120μL1×120μL\t\t\tAssay Diluent AAssay Diluent A\t\t\t1×12mL1×12mL\t\t\t\t\t\t\tDetection Reagent B\t\t\t1×120μL\t\t\tAssay Diluent B\t\t\t1×12mL\t\t\t\t\tDetection Reagent BDetection Reagent B\t\t\t1×120μL1×120μL\t\t\tAssay Diluent BAssay Diluent B\t\t\t1×12mL1×12mL\t\t\t\t\t\t\tTMB Substrate\t\t\t1×9mL\t\t\tStop Solution\t\t\t1×6mL\t\t\t\t\tTMB SubstrateTMB Substrate\t\t\t1×9mL1×9mL\t\t\tStop SolutionStop Solution\t\t\t1×6mL1×6mL\t\t\t\t\t\t\tWash Buffer (30 × concentrate)\t\t\t1×20mL\t\t\tInstruction manual\t\t\t1\t\t\t\t\tWash Buffer (30 × concentrate)Wash Buffer (30 × concentrate)\t\t\t1×20mL1×20mL\t\t\tInstruction manualInstruction manual\t\t\t11\t\t\tAssay procedure summary1. Prepare all reagents, samples and standards;
2. Add 100μL standard or sample to each well. Incubate 2 hours at 37^ooC;
3. Aspirate and add 100μL prepared Detection Reagent A. Incubate 1 hour at 37^ooC;
4. Aspirate and wash 3 times;
5. Add 100μL prepared Detection Reagent B. Incubate 30 minutes at 37^ooC;
6. Aspirate and wash 5 times;
7. Add 90μL Substrate Solution. Incubate 15-25 minutes at 37^ooC;
8. Add 50μL Stop Solution. Read at 450nm immediately.\xa0Test principle肿瘤坏死因子相关凋亡诱导配体(TRAIL)检测试剂盒肿瘤坏死因子相关凋亡诱导配体(TRAIL)检测试剂盒The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Tumor Necrosis Factor Related Apoptosis Inducing Ligand (TRAIL). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Tumor Necrosis Factor Related Apoptosis Inducing Ligand (TRAIL). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Tumor Necrosis Factor Related Apoptosis Inducing Ligand (TRAIL), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Tumor Necrosis Factor Related Apoptosis Inducing Ligand (TRAIL) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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