【OmpA】重组细菌外膜蛋白-A | Recombinant Bacterial Outer Membrane Protein-A

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艾美捷Prospec
以色列
2022-07-13 07:20

艾美捷科技有限公司

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艾美捷科技有限公司
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【产品形式】:艾美捷为您提供的【OmpA】重组细菌外膜蛋白-A蛋白产品是Sterile Filtered White lyophilized (freeze-dried) powder. It is recommended to reconstitute the lyophilized OmpA in sterile 0.4% NaHCO3.

【产品形式】:艾美捷为您提供的【OmpA】重组细菌外膜蛋白-A【OmpA】重组细菌外膜蛋白-A蛋白产品是Sterile Filtered White lyophilized (freeze-dried) powder. It is recommended to reconstitute the lyophilized OmpA in sterile 0.4% NaHCO3.

【背景资料】:The OmpA protein is one of the main outer-membrane proteins of a large array of Gram-negative bacteria such as A. salmonicida, Shigella dysenteriae and E. coli. OmpA’s major physiological functions include maintenance of the structural integrity and morphology of the cells and porin activity, as well as a role in conjugation and bacteriophage binding. Achromogenic atypical Aeromonas salmonicida is the causative agent of goldfish ulcer disease. Virulence of this bacterium is associated with the production of a paracrystalline outer membrane A-layer protein. The species specific structural gene for the monomeric form of A-protein was cloned into a pET-3d plasmid in order to express and produce a recombinant form of the protein in E. coli BL21(DE3). The induced protein was isolated from inclusion bodies by a simple solubilization-renaturation procedure and purified by ion exchange chromatography on Q-Sepharose to over 95% pure monomeric protein. Recombinant A-protein was compared by biochemical, immunological and molecular methods with the A-protein isolated from atypical A. salmonicida bacterial cells by the glycine and the membrane extraction methods.

【背景资料】:The OmpA protein is one of the main outer-membrane proteins of a large array of Gram-negative bacteria such as A. salmonicida, Shigella dysenteriae and E. coli. OmpA’s major physiological functions include maintenance of the structural integrity and morphology of the cells and porin activity, as well as a role in conjugation and bacteriophage binding. Achromogenic atypical Aeromonas salmonicida is the causative agent of goldfish ulcer disease. Virulence of this bacterium is associated with the production of a paracrystalline outer membrane A-layer protein. The species specific structural gene for the monomeric form of A-protein was cloned into a pET-3d plasmid in order to express and produce a recombinant form of the protein in E. coli BL21(DE3). The induced protein was isolated from inclusion bodies by a simple solubilization-renaturation procedure and purified by ion exchange chromatography on Q-Sepharose to over 95% pure monomeric protein. Recombinant A-protein was compared by biochemical, immunological and molecular methods with the A-protein isolated from atypical A. salmonicida bacterial cells by the glycine and the membrane extraction methods.

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以色列ProSpec-Tany公司专注于细菌来源重组蛋白质生产。其独有细菌和哺乳动物表达和蛋白折叠技术使其成为国际一流科研级蛋白供应商。ProSpec-Tany是世界上能提供蛋白种类最多公司之一。领先生产工艺和规模使其可以提供毫克到克级蛋白,价格优于同类公司。作为多家知名品牌代理,艾美捷与ProSpec一起为研究者们提供最好、最新细胞生物学研究工具而不断扩大产品及服务质量。更多特色试剂盒、特色抗体以及特色试剂等产品评论,请点击查看中国生命科学试剂产品评论网站。

以色列ProSpecProSpec-Tany公司专注于细菌来源重组蛋白质生产。其独有细菌和哺乳动物表达和蛋白折叠技术使其成为国际一流科研级蛋白供应商。ProSpec-Tany是世界上能提供蛋白种类最多公司之一。领先生产工艺和规模使其可以提供毫克到克级蛋白,价格优于同类公司。作为多家知名品牌代理,艾美捷与ProSpec一起为研究者们提供最好、最新细胞生物学研究工具而不断扩大产品及服务质量。更多特色试剂盒、特色抗体以及特色试剂等产品评论,请点击查看中国生命科学试剂产品评论中国生命科学试剂产品评论网站。

点击【OmpA】重组细菌外膜蛋白-A查看更详细产品说明,更多应用、储存、价格、货期等信息请致电400-6800-1454垂询Prospec中国区域授权总代理艾美捷科技有限公司。

点击【OmpA】重组细菌外膜蛋白-A【OmpA】重组细菌外膜蛋白-A查看更详细产品说明,更多应用、储存、价格、货期等信息请致电400-6800-1454垂询ProspecProspec中国区域授权总代理艾美捷科技有限公司。

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