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MAPK3\xa0Antibody\xa0-\xa0Product\xa0infoApplication
WBPrimary\xa0AccessionP27361Other\xa0AccessionNP_001035145.1ReactivityHuman,\xa0MouseIsotypeIgG1,kClone\xa0Names327CT18.1.2Calculated\xa0MW43136\xa0DaMAPK3\xa0Antibody\xa0-\xa0Additional\xa0infoGene\xa0ID5595Other\xa0Names
MAPK3;\xa0ERK1;\xa0PRKM3;\xa0Mitogen-activated\xa0protein\xa0kinase\xa03;\xa0ERT2;\xa0Extracellular\xa0signal-regulated\xa0kinase\xa01;\xa0Insulin-stimulated\xa0MAP2\xa0kinase;\xa0MAP\xa0kinase\xa0isoform\xa0p44;\xa0Microtubule-associated\xa0protein\xa02\xa0kinase;\xa0p44-ERK1Target/Specificity
This\xa0MAPK3\xa0monoclonal\xa0antibody\xa0is\xa0generated\xa0from\xa0mouse\xa0immunized\xa0with\xa0MAPK3\xa0recombinant\xa0protein.Dilution
WB~~1:120~1000Format
Purified\xa0monoclonal\xa0antibody\xa0supplied\xa0in\xa0PBS\xa0with\xa00.09%\xa0(W/V)\xa0sodium\xa0azide.\xa0This\xa0antibody\xa0is\xa0purified\xa0through\xa0a\xa0protein\xa0G\xa0column,\xa0eluted\xa0with\xa0high\xa0and\xa0low\xa0pH\xa0buffers\xa0and\xa0neutralized\xa0immediately,\xa0followed\xa0by\xa0dialysis\xa0against\xa0PBS.Storage
Maintain\xa0refrigerated\xa0at\xa02-8°C\xa0for\xa0up\xa0to\xa06\xa0months.\xa0For\xa0long\xa0term\xa0storage\xa0store\xa0at\xa0-20°C\xa0in\xa0small\xa0aliquots\xa0to\xa0prevent\xa0freeze-thaw\xa0cycles.Precautions
MAPK3\xa0Antibody\xa0is\xa0for\xa0research\xa0use\xa0only\xa0and\xa0not\xa0for\xa0use\xa0in\xa0diagnostic\xa0or\xa0therapeutic\xa0procedures.MAPK3\xa0Antibody\xa0-\xa0Protein\xa0InformationNameMAPK3SynonymsERK1,\xa0PRKM3Function
Serine/threonine\xa0kinase\xa0which\xa0acts\xa0as\xa0an\xa0essential\xa0component\xa0of\xa0the\xa0MAP\xa0kinase\xa0signal\xa0transduction\xa0pathway.\xa0MAPK1/ERK2\xa0and\xa0MAPK3/ERK1\xa0are\xa0the\xa02\xa0MAPKs\xa0which\xa0play\xa0an\xa0important\xa0role\xa0in\xa0the\xa0MAPK/ERK\xa0cascade.\xa0They\xa0participate\xa0also\xa0in\xa0a\xa0signaling\xa0cascade\xa0initiated\xa0by\xa0activated\xa0KIT\xa0and\xa0KITLG/SCF.\xa0Depending\xa0on\xa0the\xa0cellular\xa0context,\xa0the\xa0MAPK/ERK\xa0cascade\xa0mediates\xa0diverse\xa0biological\xa0functions\xa0such\xa0as\xa0cell\xa0growth,\xa0adhesion,\xa0survival\xa0and\xa0differentiation\xa0through\xa0the\xa0regulation\xa0of\xa0transcription,\xa0translation,\xa0cytoskeletal\xa0rearrangements.\xa0The\xa0MAPK/ERK\xa0cascade\xa0plays\xa0also\xa0a\xa0role\xa0in\xa0initiation\xa0and\xa0regulation\xa0of\xa0meiosis,\xa0mitosis,\xa0and\xa0postmitotic\xa0functions\xa0in\xa0differentiated\xa0cells\xa0by\xa0phosphorylating\xa0a\xa0number\xa0of\xa0transcription\xa0factors.\xa0About\xa0160\xa0substrates\xa0have\xa0already\xa0been\xa0discovered\xa0for\xa0ERKs.\xa0Many\xa0of\xa0these\xa0substrates\xa0are\xa0localized\xa0in\xa0the\xa0nucleus,\xa0and\xa0seem\xa0to\xa0participate\xa0in\xa0the\xa0regulation\xa0of\xa0transcription\xa0upon\xa0stimulation.\xa0However,\xa0other\xa0substrates\xa0are\xa0found\xa0in\xa0the\xa0cytosol\xa0as\xa0well\xa0as\xa0in\xa0other\xa0cellular\xa0organelles,\xa0and\xa0those\xa0are\xa0responsible\xa0for\xa0processes\xa0such\xa0as\xa0translation,\xa0mitosis\xa0and\xa0apoptosis.\xa0Moreover,\xa0the\xa0MAPK/ERK\xa0cascade\xa0is\xa0also\xa0involved\xa0in\xa0the\xa0regulation\xa0of\xa0the\xa0endosomal\xa0dynamics,\xa0including\xa0lysosome\xa0processing\xa0and\xa0endosome\xa0cycling\xa0through\xa0the\xa0perinuclear\xa0recycling\xa0compartment\xa0(PNRC);\xa0as\xa0well\xa0as\xa0in\xa0the\xa0fragmentation\xa0of\xa0the\xa0Golgi\xa0apparatus\xa0during\xa0mitosis.\xa0The\xa0substrates\xa0include\xa0transcription\xa0factors\xa0(such\xa0as\xa0ATF2,\xa0BCL6,\xa0ELK1,\xa0ERF,\xa0FOS,\xa0HSF4\xa0or\xa0SPZ1),\xa0cytoskeletal\xa0elements\xa0(such\xa0as\xa0CANX,\xa0CTTN,\xa0GJA1,\xa0MAP2,\xa0MAPT,\xa0PXN,\xa0SORBS3\xa0or\xa0STMN1),\xa0regulators\xa0of\xa0apoptosis\xa0(such\xa0as\xa0BAD,\xa0BTG2,\xa0CASP9,\xa0DAPK1,\xa0IER3,\xa0MCL1\xa0or\xa0PPARG),\xa0regulators\xa0of\xa0translation\xa0(such\xa0as\xa0EIF4EBP1)\xa0and\xa0a\xa0variety\xa0of\xa0other\xa0signaling-related\xa0molecules\xa0(like\xa0ARHGEF2,\xa0FRS2\xa0or\xa0GRB10).\xa0Protein\xa0kinases\xa0(such\xa0as\xa0RAF1,\xa0RPS6KA1/RSK1,\xa0RPS6KA3/RSK2,\xa0RPS6KA2/RSK3,\xa0RPS6KA6/RSK4,\xa0SYK,\xa0MKNK1/MNK1,\xa0MKNK2/MNK2,\xa0RPS6KA5/MSK1,\xa0RPS6KA4/MSK2,\xa0MAPKAPK3\xa0or\xa0MAPKAPK5)\xa0and\xa0phosphatases\xa0(such\xa0as\xa0DUSP1,\xa0DUSP4,\xa0DUSP6\xa0or\xa0DUSP16)\xa0are\xa0other\xa0substrates\xa0which\xa0enable\xa0the\xa0propagation\xa0the\xa0MAPK/ERK\xa0signal\xa0to\xa0additional\xa0cytosolic\xa0and\xa0nuclear\xa0targets,\xa0thereby\xa0extending\xa0the\xa0specificity\xa0of\xa0the\xa0cascade.Cellular\xa0Location
Cytoplasm.\xa0Nucleus.\xa0Note=Autophosphorylation\xa0at\xa0Thr-207\xa0promotes\xa0nuclear\xa0localization