重组小鼠粒细胞集落刺激因子/Murine G-CSF 现货重组小鼠粒细胞集落刺激因子/Murine G-CSF 现货重组小鼠粒细胞集落刺激因子/Murine G-CSF 现货重组小鼠粒细胞集落刺激因子/Murine G-CSF 现货
Recombinant Murine Granulocyte Colony-stimulating Factor

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技术参数

技术参数技术参数

FAQs

FAQsFAQs

Accession

P09920

Accession

Accession

P09920

P09920P09920

GeneID

12985

GeneID

GeneID

12985

1298512985

Source

Escherichia coli.

Source

Source

Escherichia coli.

Escherichia coli.

Molecular Weight

Approximately 18.9 kDa, a single non-glycosylated polypeptide chain containing 178 amino acids.

Molecular Weight

Molecular Weight

Approximately 18.9 kDa, a single non-glycosylated polypeptide chain containing 178 amino acids.

Approximately 18.9 kDa, a single non-glycosylated polypeptide chain containing 178 amino acids.

AA Sequence

VPLVTVSALP PSLPLPRSFL LKSLEQVRKI QASGSVLLEQ LCATYKLCHP EELVLLGHSL GIPKASLSGC SSQALQQTQC LSQLHSGLCL YQGLLQALSG ISPALAPTLD LLQLDVANFA TTIWQQMENL GVAPTVQPTQ SAMPAFTSAF QRRAGGVLAI SYLQGFLETA RLALHHLA

AA Sequence

AA Sequence

VPLVTVSALP PSLPLPRSFL LKSLEQVRKI QASGSVLLEQ LCATYKLCHP EELVLLGHSL GIPKASLSGC SSQALQQTQC LSQLHSGLCL YQGLLQALSG ISPALAPTLD LLQLDVANFA TTIWQQMENL GVAPTVQPTQ SAMPAFTSAF QRRAGGVLAI SYLQGFLETA RLALHHLA

VPLVTVSALP PSLPLPRSFL LKSLEQVRKI QASGSVLLEQ LCATYKLCHP EELVLLGHSL GIPKASLSGC SSQALQQTQC LSQLHSGLCL YQGLLQALSG ISPALAPTLD LLQLDVANFA TTIWQQMENL GVAPTVQPTQ SAMPAFTSAF QRRAGGVLAI SYLQGFLETA RLALHHLA

Purity

> 98 % by SDS-PAGE and HPLC analyses.

Purity

Purity

> 98 % by SDS-PAGE and HPLC analyses.

> 98 % by SDS-PAGE and HPLC analyses.

Biological Activity

Fully biologically active when compared to standard. The ED₅₀ as determined by a cell proliferation assay using murine NFS-60 cells is less than 0.05 ng/ml, corresponding to a specific activity of > 2.0 × 10⁷ IU/mg.

Biological Activity

Biological Activity

Fully biologically active when compared to standard. The ED₅₀ as determined by a cell proliferation assay using murine NFS-60 cells is less than 0.05 ng/ml, corresponding to a specific activity of > 2.0 × 10⁷ IU/mg.

Fully biologically active when compared to standard. The ED₅₀50 as determined by a cell proliferation assay using murine NFS-60 cells is less than 0.05 ng/ml, corresponding to a specific activity of > 2.0 × 10⁷7 IU/mg.

Physical Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Physical Appearance

Physical Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation

Lyophilized from a 0.2 μm filtered concentrated solution in 10 mM Sodium Citrate, pH 4.0, 150 mM NaCl, 0.01 % Tween-20.

Formulation

Formulation

Lyophilized from a 0.2 μm filtered concentrated solution in 10 mM Sodium Citrate, pH 4.0, 150 mM NaCl, 0.01 % Tween-20.

Lyophilized from a 0.2 μm filtered concentrated solution in 10 mM Sodium Citrate, pH 4.0, 150 mM NaCl, 0.01 % Tween-20.

Endotoxin

Less than 0.1 EU/μg of rMuG-CSF as determined by LAL method.

Endotoxin

Endotoxin

Less than 0.1 EU/μg of rMuG-CSF as determined by LAL method.

Less than 0.1 EU/μg of rMuG-CSF as determined by LAL method.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

Reconstitution

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Stability & Storage

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Usage

NOT FOR HUMAN USE.

Usage

Usage

NOT FOR HUMAN USE.

NOT FOR HUMAN USE.

SDS-PAGE

SDS-PAGE

SDS-PAGE