Recombinant Human beta-NGF/β-Nerve Growth Factor/β-NGF (CI51)

Recombinant Human beta-NGF/β-Nerve Growth Factor/β-NGF (CI51)Recombinant Human beta-NGF/β-Nerve Growth Factor/β-NGF (CI51)

产品说明(Description)

产品说明产品说明(Description)(Description)

Recombinant Human beta-Nerve Growth Factor is produced by our Mammalian expression system and the target gene encoding Ser122-Arg239 is expressed.

Recombinant Human beta-Nerve Growth Factor is produced by our Mammalian expression system and the target gene encoding Ser122-Arg239 is expressed.

Accession #: P01138

Accession #:Accession #: P01138

Known as: Beta-Nerve Growth Factor; Beta-NGF; NGF; NGFB

Known as: Known as: Beta-Nerve Growth Factor; Beta-NGF; NGF; NGFB

制剂（Formulation）

制剂（Formulation）制剂（Formulation）

Lyophilized from a 0.2 μm filtered solution of 20mM PB, 250mM NaCl, pH7.0.

Lyophilized from a 0.2 μm filtered solution of 20mM PB, 250mM NaCl, pH7.0.

质量控制（Quality Control） 

质量控制（Quality Control） 质量控制（Quality Control）

Purity: Greater than 95% as determined by reducing SDS-PAGE.

Purity: Purity: Greater than 95% as determined by reducing SDS-PAGE.

Endotoxin:  Less than 0.1 ng/ug (1 EU/ug) as determined by LAL test.

Endotoxin: Endotoxin: Less than 0.1 ng/ug (1 EU/ug) as determined by LAL test.

Bioactivity: Measured in a cell proliferation assay using TF-1 human erythroleukemic cells.

Bioactivity:Bioactivity: Measured in a cell proliferation assay using TF-1 human erythroleukemic cells.

The ED50 for this effect is 0.04-0.4 ng/mL.

The ED50 for this effect is 0.04-0.4 ng/mL.

The ED50 for this effect is typically 0.2 ng/mL.（Novoprotein）
The ED50 for this effect is typically 1.06 ng/mL. (R)

The ED50 for this effect is typically 0.2 ng/mL.（Novoprotein）
The ED50 for this effect is typically 1.06 ng/mL. (R)

复溶（Reconstitution）

复溶（Reconstitution）复溶（Reconstitution）

Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100 μg/ml.
Dissolve the lyophilized protein in distilled water.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100 μg/ml.
Dissolve the lyophilized protein in distilled water.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

保存（Storage）

保存（Storage）保存（Storage）

Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.Reconstituted protein solution can be stored at 4-7°C for 2-7 days.Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.Reconstituted protein solution can be stored at 4-7°C for 2-7 days.Aliquots of reconstituted samples are stable at < -20°C for 3 months.

背景（Background）

背景（Background）背景（Background）

Human β-Nerve Growth Factor (β-NGF) was initially isolated in the mouse submandibular gland. It is composed of three non-covalently linked subunits α, β, and γ; it exhibits all the biological activities ascribed to NGF. It is structurally related to BDNF, NT-3 and NT-4 and belongs to the cysteine-knot family of growth factors that assume stable dimeric structures. Β-NGF is a neurotrophic factor that signals through its receptor β-NGF, and plays a crucial role in the development and preservation of the sensory and sympathetic nervous systems. Β-NGF also acts as a growth and differentiation factor for B lymphocytes and enhances B-cell survival. These results suggest that β-NGF is a pleiotropic cytokine, which in addition to its neurotropic activities may have an important role in the regulation of the immune system. Human β-NGF shares 90% sequence similarity with mouse protein and shows cross-species reactivity.

Human β-Nerve Growth Factor (β-NGF) was initially isolated in the mouse submandibular gland. It is composed of three non-covalently linked subunits α, β, and γ; it exhibits all the biological activities ascribed to NGF. It is structurally related to BDNF, NT-3 and NT-4 and belongs to the cysteine-knot family of growth factors that assume stable dimeric structures. Β-NGF is a neurotrophic factor that signals through its receptor β-NGF, and plays a crucial role in the development and preservation of the sensory and sympathetic nervous systems. Β-NGF also acts as a growth and differentiation factor for B lymphocytes and enhances B-cell survival. These results suggest that β-NGF is a pleiotropic cytokine, which in addition to its neurotropic activities may have an important role in the regulation of the immune system. Human β-NGF shares 90% sequence similarity with mouse protein and shows cross-species reactivity.

电泳(SDS-PAGE)

电泳(SDS-PAGE)电泳(SDS-PAGE)

参考文献(Reference)

参考文献参考文献(Reference)(Reference)Gαq-mediated calcium dynamics and membrane tension modulate neurite plasticity[J]. Katherine M. Pearce. et al. Molecular Biology of the Cell. 2019.

SORT1 mutation resulting in sortilin deficiency and p75NTR upregulation in a family with essential tremor[J]. Sánchez E. et al. ASN neuro. 2015.

SORT1 mutation resulting in sortilin deficiency and p75NTR upregulation in a family with essential tremor[J]. Sánchez E. et al. ASN neuro.et al. ASN neuro. 2015.

FOR RESEARCH USE ONLY

FOR RESEARCH USE ONLYFOR RESEARCH USE ONLYFOR RESEARCH USE ONLY