• 全细胞亚硫酸盐修饰试剂盒详细信息：
• Product Overview
  
  The Methylamp™ Whole Cell Bisulfite Modification Kit is an innovative and unique set of essential components which enables the experimenter to perform DNA methylation analysis and modify DNA directly from cells or tissues using Epigenteks uniquely simplified and streamlined bisulfite method. The entire procedure can be completed within only 3 hours. The Methylamp™ Whole Cell Bisulfite Modification Kit is specifically designed for DNA methylation research using minute amounts of starting materials including cells cultured in 96-well/384 well plates, tissue section samples, microdissection samples, tissue biopsy and early embryonic cells/oocytes. 
  
  Eluted modified DNA by using the Methylamp™ Whole Cell Bisulfite Modification Kit is suited for real time MS-PCR. It is also suitable for all techniques currently used for the analysis of DNA methylation; including conventional MS-PCR, bisulfite sequencing, pyrosequencing, and methylation microarray. If you use the Methylamp™ Whole Cell Bisulfite Modification Kit for MSP, the numbers of PCR cycles should be greater than 45. The amount of starting materials for each modification can be 100-20000 cells, or 1 µg-100 µg tissues, or 0.2-2 mm2 tissue section samples. For optimal modification, the amount should be 500- 5000 cells, or 5-20 µg tissues, or 0.5-1 mm2 tissue section samples, respectively.
  
  WHY CHOOSE THE METHYLAMP™ DNA MODIFICATION KIT?
  • Proprietary and unique by directly modifying cells and tissues.
  • Rapid streamlined 3 hour procedure from cells/tissues to modified DNA.
  • Completely converts unmethylated cytosine into uracil: modified DNA > 99.5%.
  • The lowest degradation of DNA in the modification process: more than 90% of DNA loss can be prevented with a unique DNA protection buffer.
  全细胞亚硫酸盐修饰试剂盒Principle & Procedure

  
  The Methylamp™ Whole Cell Bisulfite Modification Kit contains all reagents required for bisulfite conversion directly on a cell or tissue sample. The kit allows DNA to be isolated from cells or tissues, denatured and bisulfite modified simultaneously in same tube with the specific reaction buffer under the thermodynamic condition. In the modification process, bisulfite reagent reacts specifically with single-stranded DNA, thereby deaminating cytosine and creating a uracil residue. The unique DNA protection reagents contained in the modification buffer can prevent the chemical and thermophilic degradation of DNA in the bisulfite treatment. The non-toxic modified DNA capture buffer enables DNA to tightly bind to the column filter, thus DNA cleaning can be carried out on the column to effectively remove residual sodium bisulfite and salts. Modified DNA can then be eluted and stably stored at –20°C for up to 2 months.
  

  SCHEMATIC PROCEDURE:
  	The different amounts of MCF-7 cells or DNA isolated from MCF-7 cells were modified using the Methylamp™ Whole Cell Bisulfite Modification Kit or Methylamp™ One-Step DNA modification kit, respectively. 10 µl of modified DNA were eluted and 2 µl of elution were used in real time PCR. A pair of primers and a probe designed to amplify both methylated and unmethylated alleles of β-actin was used.

  全细胞亚硫酸盐修饰试剂盒Product Components

  
  W1 (Digestion powder)
  W2 (Digestion solution)
  W3 (Cell collection buffer)
  W4 (DNA modification powder)
  W5 (DNA modification buffer)
  W6 (Balance buffer)
  W7 (DNA binding buffer)
  W8 (modified DNA elution)
  F-spin column
  F-collection tube
  User guide
  81215B-20 一站式免疫印迹套装
  100928-20 移湿法电转缓冲液
  100929-20 半干法电转缓冲液
  81210-200 Western 印迹膜封膜液
  81208-100 Western Blot抗体稀释液
  80813-50 Western印迹膜抗体清除剂
  80813-200 Western印迹膜抗体清除剂
  81223-100 增强型DAB显色试剂盒
  81224-100 BCIP/NBT底物显色试剂盒
  100214-100 预混型BCIP/NBT溶液
  91101A-100 一管式TMB显色液
  91101B-100 一管式TMB显色液
  100887-100 BCIP
  100888-250 NBT
  100930-1 硝酸纤维素膜
  100929-20 尼龙膜
  100878-1 PVDF膜
  其它
  61205-15 白蛋白清除剂
  81216-1.5 核酸清除剂
  80808-1 蛋白酶抑制剂混合液
  100837 苯（PMSF）
  100845 胰蛋白酶抑制剂
  100846 胃蛋白酶抑制剂
  100847 亮抑蛋白酶肽
  100832 EDTA溶液
  80809-1 蛋白磷酸酶抑制剂混合液
  100806 Earle溶液
  91105A-100 Hanks溶液
  100927-100 D-Hanks溶液
  100215-250 PBS缓冲液，10×
  100807 杜氏PBS缓冲液，10×
  90306A-250 胰酶消化液
  90306B-250 胰酶消化液
  90307-100 青链霉素溶液，100×

  全细胞亚硫酸盐修饰试剂盒User Guide & MSDS

全细胞亚硫酸盐修饰试剂盒详细信息：

全细胞亚硫酸盐修饰试剂盒详细信息：

Product Overview

The Methylamp™ Whole Cell Bisulfite Modification Kit is an innovative and unique set of essential components which enables the experimenter to perform DNA methylation analysis and modify DNA directly from cells or tissues using Epigenteks uniquely simplified and streamlined bisulfite method. The entire procedure can be completed within only 3 hours. The Methylamp™ Whole Cell Bisulfite Modification Kit is specifically designed for DNA methylation research using minute amounts of starting materials including cells cultured in 96-well/384 well plates, tissue section samples, microdissection samples, tissue biopsy and early embryonic cells/oocytes. 

Eluted modified DNA by using the Methylamp™ Whole Cell Bisulfite Modification Kit is suited for real time MS-PCR. It is also suitable for all techniques currently used for the analysis of DNA methylation; including conventional MS-PCR, bisulfite sequencing, pyrosequencing, and methylation microarray. If you use the Methylamp™ Whole Cell Bisulfite Modification Kit for MSP, the numbers of PCR cycles should be greater than 45. The amount of starting materials for each modification can be 100-20000 cells, or 1 µg-100 µg tissues, or 0.2-2 mm2 tissue section samples. For optimal modification, the amount should be 500- 5000 cells, or 5-20 µg tissues, or 0.5-1 mm2 tissue section samples, respectively.

WHY CHOOSE THE METHYLAMP™ DNA MODIFICATION KIT?

• Proprietary and unique by directly modifying cells and tissues.
• Rapid streamlined 3 hour procedure from cells/tissues to modified DNA.
• Completely converts unmethylated cytosine into uracil: modified DNA > 99.5%.
• The lowest degradation of DNA in the modification process: more than 90% of DNA loss can be prevented with a unique DNA protection buffer.

全细胞亚硫酸盐修饰试剂盒Principle & Procedure

The Methylamp™ Whole Cell Bisulfite Modification Kit contains all reagents required for bisulfite conversion directly on a cell or tissue sample. The kit allows DNA to be isolated from cells or tissues, denatured and bisulfite modified simultaneously in same tube with the specific reaction buffer under the thermodynamic condition. In the modification process, bisulfite reagent reacts specifically with single-stranded DNA, thereby deaminating cytosine and creating a uracil residue. The unique DNA protection reagents contained in the modification buffer can prevent the chemical and thermophilic degradation of DNA in the bisulfite treatment. The non-toxic modified DNA capture buffer enables DNA to tightly bind to the column filter, thus DNA cleaning can be carried out on the column to effectively remove residual sodium bisulfite and salts. Modified DNA can then be eluted and stably stored at –20°C for up to 2 months.

SCHEMATIC PROCEDURE:
	The different amounts of MCF-7 cells or DNA isolated from MCF-7 cells were modified using the Methylamp™ Whole Cell Bisulfite Modification Kit or Methylamp™ One-Step DNA modification kit, respectively. 10 µl of modified DNA were eluted and 2 µl of elution were used in real time PCR. A pair of primers and a probe designed to amplify both methylated and unmethylated alleles of β-actin was used.

全细胞亚硫酸盐修饰试剂盒Product Components

W1 (Digestion powder)
W2 (Digestion solution)
W3 (Cell collection buffer)
W4 (DNA modification powder)
W5 (DNA modification buffer)
W6 (Balance buffer)
W7 (DNA binding buffer)
W8 (modified DNA elution)
F-spin column
F-collection tube
User guide
81215B-20 一站式免疫印迹套装
100928-20 移湿法电转缓冲液
100929-20 半干法电转缓冲液
81210-200 Western 印迹膜封膜液
81208-100 Western Blot抗体稀释液
80813-50 Western印迹膜抗体清除剂
80813-200 Western印迹膜抗体清除剂
81223-100 增强型DAB显色试剂盒
81224-100 BCIP/NBT底物显色试剂盒
100214-100 预混型BCIP/NBT溶液
91101A-100 一管式TMB显色液
91101B-100 一管式TMB显色液
100887-100 BCIP
100888-250 NBT
100930-1 硝酸纤维素膜
100929-20 尼龙膜
100878-1 PVDF膜
其它
61205-15 白蛋白清除剂
81216-1.5 核酸清除剂
80808-1 蛋白酶抑制剂混合液
100837 苯（PMSF）
100845 胰蛋白酶抑制剂
100846 胃蛋白酶抑制剂
100847 亮抑蛋白酶肽
100832 EDTA溶液
80809-1 蛋白磷酸酶抑制剂混合液
100806 Earle溶液
91105A-100 Hanks溶液
100927-100 D-Hanks溶液
100215-250 PBS缓冲液，10×
100807 杜氏PBS缓冲液，10×
90306A-250 胰酶消化液
90306B-250 胰酶消化液
90307-100 青链霉素溶液，100×

全细胞亚硫酸盐修饰试剂盒User Guide & MSDS

Product Overview
The Methylamp™ Whole Cell Bisulfite Modification KitMethylamp™ Whole Cell Bisulfite Modification Kit is an innovative and unique set of essential components which enables the experimenter to perform DNA methylation analysis and modify DNA directly from cells or tissues using Epigenteks uniquely simplified and streamlined bisulfite method. The entire procedure can be completed within only 3 hours. The Methylamp™ Whole Cell Bisulfite Modification Kit is specifically designed for DNA methylation research using minute amounts of starting materials including cells cultured in 96-well/384 well plates, tissue section samples, microdissection samples, tissue biopsy and early embryonic cells/oocytes. 

Eluted modified DNA by using the Methylamp™ Whole Cell Bisulfite Modification Kit is suited for real time MS-PCR. It is also suitable for all techniques currently used for the analysis of DNA methylation; including conventional MS-PCR, bisulfite sequencing, pyrosequencing, and methylation microarray. If you use the Methylamp™ Whole Cell Bisulfite Modification Kit for MSP, the numbers of PCR cycles should be greater than 45. The amount of starting materials for each modification can be 100-20000 cells, or 1 µg-100 µg tissues, or 0.2-2 mm2 tissue section samples. For optimal modification, the amount should be 500- 5000 cells, or 5-20 µg tissues, or 0.5-1 mm2 tissue section samples, respectively.

WHY CHOOSE THE METHYLAMP™ DNA MODIFICATION KIT?WHY CHOOSE THE METHYLAMP™ DNA MODIFICATION KIT?

• Proprietary and unique by directly modifying cells and tissues.
• Rapid streamlined 3 hour procedure from cells/tissues to modified DNA.
• Completely converts unmethylated cytosine into uracil: modified DNA > 99.5%.
• The lowest degradation of DNA in the modification process: more than 90% of DNA loss can be prevented with a unique DNA protection buffer.

Proprietary and unique by directly modifying cells and tissues.

Proprietary and unique by directly modifying cells and tissues.

Rapid streamlined 3 hour procedure from cells/tissues to modified DNA.

Rapid streamlined 3 hour procedure from cells/tissues to modified DNA.

Completely converts unmethylated cytosine into uracil: modified DNA > 99.5%.

Completely converts unmethylated cytosine into uracil: modified DNA > 99.5%.

The lowest degradation of DNA in the modification process: more than 90% of DNA loss can be prevented with a unique DNA protection buffer.

The lowest degradation of DNA in the modification process: more than 90% of DNA loss can be prevented with a unique DNA protection buffer.全细胞亚硫酸盐修饰试剂盒Principle & Procedure

The Methylamp™ Whole Cell Bisulfite Modification Kit contains all reagents required for bisulfite conversion directly on a cell or tissue sample. The kit allows DNA to be isolated from cells or tissues, denatured and bisulfite modified simultaneously in same tube with the specific reaction buffer under the thermodynamic condition. In the modification process, bisulfite reagent reacts specifically with single-stranded DNA, thereby deaminating cytosine and creating a uracil residue. The unique DNA protection reagents contained in the modification buffer can prevent the chemical and thermophilic degradation of DNA in the bisulfite treatment. The non-toxic modified DNA capture buffer enables DNA to tightly bind to the column filter, thus DNA cleaning can be carried out on the column to effectively remove residual sodium bisulfite and salts. Modified DNA can then be eluted and stably stored at –20°C for up to 2 months.

The Methylamp™ Whole Cell Bisulfite Modification Kit contains all reagents required for bisulfite conversion directly on a cell or tissue sample. The kit allows DNA to be isolated from cells or tissues, denatured and bisulfite modified simultaneously in same tube with the specific reaction buffer under the thermodynamic condition. In the modification process, bisulfite reagent reacts specifically with single-stranded DNA, thereby deaminating cytosine and creating a uracil residue. The unique DNA protection reagents contained in the modification buffer can prevent the chemical and thermophilic degradation of DNA in the bisulfite treatment. The non-toxic modified DNA capture buffer enables DNA to tightly bind to the column filter, thus DNA cleaning can be carried out on the column to effectively remove residual sodium bisulfite and salts. Modified DNA can then be eluted and stably stored at –20°C for up to 2 months.

SCHEMATIC PROCEDURE:
	The different amounts of MCF-7 cells or DNA isolated from MCF-7 cells were modified using the Methylamp™ Whole Cell Bisulfite Modification Kit or Methylamp™ One-Step DNA modification kit, respectively. 10 µl of modified DNA were eluted and 2 µl of elution were used in real time PCR. A pair of primers and a probe designed to amplify both methylated and unmethylated alleles of β-actin was used.

SCHEMATIC PROCEDURE:
The different amounts of MCF-7 cells or DNA isolated from MCF-7 cells were modified using the Methylamp™ Whole Cell Bisulfite Modification Kit or Methylamp™ One-Step DNA modification kit, respectively. 10 µl of modified DNA were eluted and 2 µl of elution were used in real time PCR. A pair of primers and a probe designed to amplify both methylated and unmethylated alleles of β-actin was used.SCHEMATIC PROCEDURE:SCHEMATIC PROCEDURE:SCHEMATIC PROCEDURE:SCHEMATIC PROCEDURE:
The different amounts of MCF-7 cells or DNA isolated from MCF-7 cells were modified using the Methylamp™ Whole Cell Bisulfite Modification Kit or Methylamp™ One-Step DNA modification kit, respectively. 10 µl of modified DNA were eluted and 2 µl of elution were used in real time PCR. A pair of primers and a probe designed to amplify both methylated and unmethylated alleles of β-actin was used.
The different amounts of MCF-7 cells or DNA isolated from MCF-7 cells were modified using the Methylamp™ Whole Cell Bisulfite Modification Kit or Methylamp™ One-Step DNA modification kit, respectively. 10 µl of modified DNA were eluted and 2 µl of elution were used in real time PCR. A pair of primers and a probe designed to amplify both methylated and unmethylated alleles of β-actin was used.
The different amounts of MCF-7 cells or DNA isolated from MCF-7 cells were modified using the Methylamp™ Whole Cell Bisulfite Modification Kit or Methylamp™ One-Step DNA modification kit, respectively. 10 µl of modified DNA were eluted and 2 µl of elution were used in real time PCR. A pair of primers and a probe designed to amplify both methylated and unmethylated alleles of β-actin was used.全细胞亚硫酸盐修饰试剂盒Product Components

W1 (Digestion powder)
W2 (Digestion solution)
W3 (Cell collection buffer)
W4 (DNA modification powder)
W5 (DNA modification buffer)
W6 (Balance buffer)
W7 (DNA binding buffer)
W8 (modified DNA elution)
F-spin column
F-collection tube
User guide
81215B-20 一站式免疫印迹套装
100928-20 移湿法电转缓冲液
100929-20 半干法电转缓冲液
81210-200 Western 印迹膜封膜液
81208-100 Western Blot抗体稀释液
80813-50 Western印迹膜抗体清除剂
80813-200 Western印迹膜抗体清除剂
81223-100 增强型DAB显色试剂盒
81224-100 BCIP/NBT底物显色试剂盒
100214-100 预混型BCIP/NBT溶液
91101A-100 一管式TMB显色液
91101B-100 一管式TMB显色液
100887-100 BCIP
100888-250 NBT
100930-1 硝酸纤维素膜
100929-20 尼龙膜
100878-1 PVDF膜
其它
61205-15 白蛋白清除剂
81216-1.5 核酸清除剂
80808-1 蛋白酶抑制剂混合液
100837 苯（PMSF）
100845 胰蛋白酶抑制剂
100846 胃蛋白酶抑制剂
100847 亮抑蛋白酶肽
100832 EDTA溶液
80809-1 蛋白磷酸酶抑制剂混合液
100806 Earle溶液
91105A-100 Hanks溶液
100927-100 D-Hanks溶液
100215-250 PBS缓冲液，10×
100807 杜氏PBS缓冲液，10×
90306A-250 胰酶消化液
90306B-250 胰酶消化液
90307-100 青链霉素溶液，100×

W1 (Digestion powder)
W2 (Digestion solution)
W3 (Cell collection buffer)
W4 (DNA modification powder)
W5 (DNA modification buffer)
W6 (Balance buffer)
W7 (DNA binding buffer)
W8 (modified DNA elution)
F-spin column
F-collection tube
User guide
81215B-20 一站式免疫印迹套装
100928-20 移湿法电转缓冲液
100929-20 半干法电转缓冲液
81210-200 Western 印迹膜封膜液
81208-100 Western Blot抗体稀释液
80813-50 Western印迹膜抗体清除剂
80813-200 Western印迹膜抗体清除剂
81223-100 增强型DAB显色试剂盒
81224-100 BCIP/NBT底物显色试剂盒
100214-100 预混型BCIP/NBT溶液
91101A-100 一管式TMB显色液
91101B-100 一管式TMB显色液
100887-100 BCIP
100888-250 NBT
100930-1 硝酸纤维素膜
100929-20 尼龙膜
100878-1 PVDF膜
其它
61205-15 白蛋白清除剂
81216-1.5 核酸清除剂
80808-1 蛋白酶抑制剂混合液
100837 苯（PMSF）
100845 胰蛋白酶抑制剂
100846 胃蛋白酶抑制剂
100847 亮抑蛋白酶肽
100832 EDTA溶液
80809-1 蛋白磷酸酶抑制剂混合液
100806 Earle溶液
91105A-100 Hanks溶液
100927-100 D-Hanks溶液
100215-250 PBS缓冲液，10×
100807 杜氏PBS缓冲液，10×
90306A-250 胰酶消化液
90306B-250 胰酶消化液
90307-100 青链霉素溶液，100×全细胞亚硫酸盐修饰试剂盒User Guide & MSDS