特别声明：本产品及我公司所售其他产品均为科研类试剂产品，严禁用于药物、医疗及其他非科研用途。

特别声明：本产品及我公司所售其他产品均为科研类试剂产品，严禁用于药物、医疗及其他非科研用途。

北京百奥莱博主要从事生物试剂的生产和销*(代"售")，产品线涵盖N端Myc标签融合蛋白质粒厂家价格在内的分子生物学试剂产品，还包括PCR及荧光定量PCR、核酸提取试剂盒类、样本核酸保护试剂、核酸提取、克隆与转化、蛋白纯化、蛋白检测、免疫组化、细胞凋亡与抗体等。

北京百奥莱博主要从事生物试剂的生产和销*(代"售")，产品线涵盖N端Myc标签融合蛋白质粒厂家价格在内的分子生物学试剂产品，还包括PCR及荧光定量PCR、核酸提取试剂盒类、样本核酸保护试剂、核酸提取、克隆与转化、蛋白纯化、蛋白检测、免疫组化、细胞凋亡与抗体等。

名称：N端Myc标签融合蛋白质粒厂家价格
产地：国产|进口
品牌：百奥莱博
英文名：N-Myc plasmid（with CMV promoter）
本质粒是用于在哺乳动物细胞中表达N端和Myc tag(Myc标签)融合的目的蛋白的表达质粒。含有CMV启动子可以高效启动目的蛋白在细胞中的表达；在多克隆位点的5"端含有一个可以编码Myc标签的序列，因此可以表达出含有Myc标签的融合蛋白，可以方便地使用抗Myc的抗体来识别目的蛋白，有利于目的蛋白检测和分离纯化。质粒为卡那霉素抗性。转染细胞后，可使用G418筛选稳定表达目的蛋白的细胞株。

本质粒质粒的主要信息如下：

名称：N端Myc标签融合蛋白质粒厂家价格N端Myc标签融合蛋白质粒厂家价格N端Myc标签融合蛋白质粒厂家价格
产地：国产|进口
品牌：百奥莱博
英文名：N-Myc plasmid（with CMV promoter）
本质粒是用于在哺乳动物细胞中表达N端和Myc tag(Myc标签)融合的目的蛋白的表达质粒。含有CMV启动子可以高效启动目的蛋白在细胞中的表达；在多克隆位点的5"端含有一个可以编码Myc标签的序列，因此可以表达出含有Myc标签的融合蛋白，可以方便地使用抗Myc的抗体来识别目的蛋白，有利于目的蛋白检测和分离纯化。质粒为卡那霉素抗性。转染细胞后，可使用G418筛选稳定表达目的蛋白的细胞株。

本质粒质粒的主要信息如下：

Feature Nucleotide	Position
CMV promoter	1-602
T3 promoter and T3 primer binding site	620-639
Myc tag	679-708
multiple cloning site	711-784
T7 promoter and T7 primer binding site	827-848
SV40 polyA signal	860-1243
f1 origin of ss-DNA replication	1381-1687
bla promoter	1712-1836
SV40 promoter	1856-2194
neomycin/kanamycin resistance ORF	2229-3020
HSV-thymidine kinase(TK)polyA signal	3021-3479
pUC origin	3608-4275

Feature NucleotidePositionCMV promoter1-602T3 promoter and T3 primer binding site620-639Myc tag679-708multiple cloning site711-784T7 promoter and T7 primer binding site827-848SV40 polyA signal860-1243f1 origin of ss-DNA replication1381-1687bla promoter1712-1836SV40 promoter1856-2194neomycin/kanamycin resistance ORF2229-3020HSV-thymidine kinase(TK)polyA signal3021-3479pUC origin3608-4275Feature NucleotidePositionFeature NucleotideFeature NucleotidePositionPositionCMV promoter1-602CMV promoterCMV promoter1-6021-602T3 promoter and T3 primer binding site620-639T3 promoter and T3 primer binding siteT3 promoter and T3 primer binding site620-639620-639Myc tag679-708Myc tagMyc tag679-708679-708multiple cloning site711-784multiple cloning sitemultiple cloning site711-784711-784T7 promoter and T7 primer binding site827-848T7 promoter and T7 primer binding siteT7 promoter and T7 primer binding site827-848827-848SV40 polyA signal860-1243SV40 polyA signalSV40 polyA signal860-1243860-1243f1 origin of ss-DNA replication1381-1687f1 origin of ss-DNA replicationf1 origin of ss-DNA replication1381-16871381-1687bla promoter1712-1836bla promoterbla promoter1712-18361712-1836SV40 promoter1856-2194SV40 promoterSV40 promoter1856-21941856-2194neomycin/kanamycin resistance ORF2229-3020neomycin/kanamycin resistance ORFneomycin/kanamycin resistance ORF2229-30202229-3020HSV-thymidine kinase(TK)polyA signal3021-3479HSV-thymidine kinase(TK)polyA signalHSV-thymidine kinase(TK)polyA signal3021-34793021-3479pUC origin3608-4275pUC originpUC origin3608-42753608-4275

本质粒的图谱如下：


使用说明：
1. 首次使用时请先取少量本质粒转化大肠杆菌，进行质粒小量、中量或大量抽提后再用于后续用途。抽提获得的质粒可以通过酶切电泳进行鉴定，或通过测序进行鉴定。
2. 本质粒在其多克隆位点适当酶切后可以插入待表达的目的基因，需注意插入基因片段和tag之间的读码框要一致，即需要避免发生移码突变。构建的质粒可以用常规方法转染细胞。

储存条件：-20℃。

关于N端Myc标签融合蛋白质粒厂家价格N端Myc标签融合蛋白质粒厂家价格N端Myc标签融合蛋白质粒厂家价格的更详细说明，请致电我公司咨询，我们将以最饱满的热情为您提供解答，此外，我公司专业供应下列产品：

·兔抗α-Tubulin抗体
编号：YT671
英文名称：α-Tubulin Rabbit Polyclonal Antibody
规格：100μl
本品为来源于兔的抗α-Tubulin的多克隆抗体
宿主：兔
用途：WB,ICC, IHC
交叉反应性：人，小鼠，大鼠
分子量：～50KDa
推荐稀释比例：WB(1:1000),IF(1:100),IHC(1:50),ICC(1:50)

About this Antibody
Name	α-Tubulin Rabbit Polyclonal Antibody
Category	Polyclonal antibody (pAb); Primary antibody
Isotype	IgG
Purification	Affinity purification
About the Immunogen
Immunogen	This antibody is produced by immunizing rabbits with a synthetic peptide (KLH-coupled) corresponding to C-terminal α-Tubulin.
Gene ID	7277
SwissProt	P68366
Synonyms	ALS22;TUBA1;H2-ALPHA
Category	Cell Cycle/Organism-specific biosystem
Background	The cytoskeleton consists of three types of cytosolic fibers: microtubules, microfilaments (actin filaments), and intermediate filaments. Globular Tubulin subunits comprise the microtubule building block, with α/β-Tubulin heterodimers forming the Tubulin subunit common to all eukaryotic cells. Acetylation of alpha chains at Lys-40 stabilizes microtubules and affects affinity and processivity of microtubule motors. This modification has a role in multiple cellular functions, ranging from cell motility, cell cycle progression or cell differentiation to intracellular trafficking and signaling.

About this Antibody

Name

α-Tubulin Rabbit Polyclonal Antibody

Category

Polyclonal antibody (pAb); Primary antibody

Isotype

IgG

Purification

Affinity purification

About the Immunogen

Immunogen

This antibody is produced by immunizing rabbits with a synthetic peptide (KLH-coupled) corresponding to C-terminal α-Tubulin.

Gene ID

7277

SwissProt

P68366

Synonyms

ALS22;TUBA1;H2-ALPHA

Category

Cell Cycle/Organism-specific biosystem

Background

The cytoskeleton consists of three types of cytosolic fibers: microtubules, microfilaments (actin filaments), and intermediate filaments. Globular Tubulin subunits comprise the microtubule building block, with α/β-Tubulin heterodimers forming the Tubulin subunit common to all eukaryotic cells. Acetylation of alpha chains at Lys-40 stabilizes microtubules and affects affinity and processivity of microtubule motors. This modification has a role in multiple cellular functions, ranging from cell motility, cell cycle progression or cell differentiation to intracellular trafficking and signaling.

About this Antibody

About this Antibody

About this Antibody

About this AntibodyAbout this Antibody

Name

α-Tubulin Rabbit Polyclonal Antibody

Name

Name

Name

α-Tubulin Rabbit Polyclonal Antibody

α-Tubulin Rabbit Polyclonal Antibody

α-Tubulin Rabbit Polyclonal Antibody

Category

Polyclonal antibody (pAb); Primary antibody

Category

Category

Category

Polyclonal antibody (pAb); Primary antibody

Polyclonal antibody (pAb); Primary antibody

Polyclonal antibody (pAb); Primary antibody

Isotype

IgG

Isotype

Isotype

Isotype

IgG

IgG

IgG

Purification

Affinity purification

Purification

Purification

Purification

Affinity purification

Affinity purification

Affinity purification

About the Immunogen

About the Immunogen

About the Immunogen

About the ImmunogenAbout the Immunogen

Immunogen

This antibody is produced by immunizing rabbits with a synthetic peptide (KLH-coupled) corresponding to C-terminal α-Tubulin.

Immunogen

Immunogen

Immunogen

This antibody is produced by immunizing rabbits with a synthetic peptide (KLH-coupled) corresponding to C-terminal α-Tubulin.

This antibody is produced by immunizing rabbits with a synthetic peptide (KLH-coupled) corresponding to C-terminal α-Tubulin.

This antibody is produced by immunizing rabbits with a synthetic peptide (KLH-coupled) corresponding to C-terminal α-Tubulin.

Gene ID

7277

Gene ID

Gene ID

Gene ID

7277

7277

7277

SwissProt

P68366

SwissProt

SwissProt

SwissProt

P68366

P68366

P68366

Synonyms

ALS22;TUBA1;H2-ALPHA

Synonyms

Synonyms

Synonyms

ALS22;TUBA1;H2-ALPHA

ALS22;TUBA1;H2-ALPHA

ALS22;TUBA1;H2-ALPHA

Category

Cell Cycle/Organism-specific biosystem

Category

Category

Category

Cell Cycle/Organism-specific biosystem

Cell Cycle/Organism-specific biosystem

Cell Cycle/Organism-specific biosystem

Background

The cytoskeleton consists of three types of cytosolic fibers: microtubules, microfilaments (actin filaments), and intermediate filaments. Globular Tubulin subunits comprise the microtubule building block, with α/β-Tubulin heterodimers forming the Tubulin subunit common to all eukaryotic cells. Acetylation of alpha chains at Lys-40 stabilizes microtubules and affects affinity and processivity of microtubule motors. This modification has a role in multiple cellular functions, ranging from cell motility, cell cycle progression or cell differentiation to intracellular trafficking and signaling.

Background

Background

Background

The cytoskeleton consists of three types of cytosolic fibers: microtubules, microfilaments (actin filaments), and intermediate filaments. Globular Tubulin subunits comprise the microtubule building block, with α/β-Tubulin heterodimers forming the Tubulin subunit common to all eukaryotic cells. Acetylation of alpha chains at Lys-40 stabilizes microtubules and affects affinity and processivity of microtubule motors. This modification has a role in multiple cellular functions, ranging from cell motility, cell cycle progression or cell differentiation to intracellular trafficking and signaling.

The cytoskeleton consists of three types of cytosolic fibers: microtubules, microfilaments (actin filaments), and intermediate filaments. Globular Tubulin subunits comprise the microtubule building block, with α/β-Tubulin heterodimers forming the Tubulin subunit common to all eukaryotic cells. Acetylation of alpha chains at Lys-40 stabilizes microtubules and affects affinity and processivity of microtubule motors. This modification has a role in multiple cellular functions, ranging from cell motility, cell cycle progression or cell differentiation to intracellular trafficking and signaling.

The cytoskeleton consists of three types of cytosolic fibers: microtubules, microfilaments (actin filaments), and intermediate filaments. Globular Tubulin subunits comprise the microtubule building block, with α/β-Tubulin heterodimers forming the Tubulin subunit common to all eukaryotic cells. Acetylation of alpha chains at Lys-40 stabilizes microtubules and affects affinity and processivity of microtubule motors. This modification has a role in multiple cellular functions, ranging from cell motility, cell cycle progression or cell differentiation to intracellular trafficking and signaling.

注意事项：
1. 如果本抗体用于Western blot (WB)、免疫荧光(IF)、免疫细胞化学(ICC)等实验，请注意回收使用过的稀释抗体 回收的抗体通常至少可以重复使用5-10次。稀释后的抗体，包括已经使用过的稀释抗体，请4℃保存。
2. 回收后重复使用的抗体，使用方法同新鲜稀释的抗体。如果在重复使用过程中发现抗体出现轻微混浊现象，可以10,000g离心1-3分钟，取上清用于后续检测。如果回收的抗体出现明显的絮状物或长霉长菌等情况，则可以考虑废弃该抗体。
3. 提供的Western一抗稀释液也可以用于免疫荧光(IF)、免疫组化(IHC)、免疫细胞化学(ICC)等适当用途。如果希望获得最佳的检测效果，请考虑使用上述检测专用的一抗稀释液。

储存条件：-20℃，有效期一年。


N端Myc标签融合蛋白质粒厂家价格N端Myc标签融合蛋白质粒厂家价格N端Myc标签融合蛋白质粒厂家价格关键词：N-Myc plasmid（with CMV promoter）,N端Myc标签融合蛋白质粒,YT482


·CREB凝胶迁移突变探针(10μM)
编号：YT222
英文名称：CREB Mutation Probe For EMSA(10μM)
规格：30μl
本探针是用于EMSA(也称gel shift)研究的CREB consensus oligonucleotide的突变体。可以作为EMSA探针－CREB的阴性对照，用于EMSA结合反应中突变探针的冷竞争反应等。一个包装的突变探针，如果用于同位素标记EMSA探针的突变探针冷竞争反应，可以进行90-180个突变探针的冷竞争反应。如果用于生物素标记探针的冷竞争时，可以进行约30个冷竞争反应。

CREB consensus oligo的序列如下：
5"-AGA GAT TGC CTG ACG TCA GAG AGC TAG-3"
3"-TCT CTA ACG GAC TGC AGT CTC TCG ATC-5"

EMSA突变探针－CREB中CREB的公认的结合位点发生了突变，从而使CREB无法和该突变探针结合。在探针冷竞争反应中，正常的标记探针和CREB的结合的条带会被抑制；而在突变探针冷竞争反应(cold competition)中，正常的标记探针和CREB的结合的条带不会被抑制。参考下图。


一个典型的EMSA/Gel- Shift分析图
1,阴性对照反应(标记探针)；
2,常规反应(含激活的目的转录因子的核蛋白＋标记探针)；
3,探针冷竞争反应(含激活的目的转录因子的核蛋白＋标记探针＋标记探针100倍量的未标记探针)；
4,突变探针的冷竞争反应(含激活的目的转录因子的核蛋白＋标记探针＋标记探针100倍量的未标记突变探针)；
5,Super-shift反应(含激活的目的转录因子的核蛋白＋标记探针＋目的转录因子的特异抗体)。

注意事项：
1. 避免加热到40℃以上，温度过高会导致双链DNA探针解聚成单链。而单链无法用于EMSA研究。
2. 对于EMSA的详细操作可以参考我们的EMSA试剂盒的使用说明。


N端Myc标签融合蛋白质粒厂家价格N端Myc标签融合蛋白质粒厂家价格N端Myc标签融合蛋白质粒厂家价格关键词：N-Myc plasmid（with CMV promoter）,N端Myc标签融合蛋白质粒,YT482

0030011 冻干粉兔血浆
对甲*磺酸乙酯 Potassium carbonate sesquihydrate 80-40-0
阿特拉津 Ceftriaxone sodiu*(代"m") 1912-24-9
ARB11713 人链激酶(SK)ELISA检测服务 Human streptokinase,sk ELISA KIT
ARB13204 小鼠脑肠肽(BGP/GehrelIn)elisa测定使用说明书 Mouse brain-gut Peptides,bgp/gehrelin ELISA KIT
ARB10565 人过氧化物酶体增殖物激活受体α(PPAR-α)ELISA代测服务 Human peroxisome prolifeRators activator receptors alpha,ppar-α ELISA KIT
ARB13591 兔子纤溶酶原激活物抑制因子(PAI)免费代测 Rabbit plasminogen activator inhibitor,pai ELISA KIT
苏木色精 Glutaric acid 517-28-2
ARB10024 人5羟色胺(5-HT)含量分析 Human 5-hydroxytryptamine,5ht ELISA KIT
ARB13132 小鼠胆固醇酯转移蛋白(CETP)Elisa分析 Mouse cholest erolester transfer protein,cetp ELISA KIT
2-噻*(代"吩")甲酰三*丙*(代"酮") DL-Theronine 326-91-0
147-85-3 L-Proline L-脯*酸
原位杂交A-B显影液 2×500ml

我公司正在优惠促销克隆与表达等系列产品，期待您的咨询选购N端Myc标签融合蛋白质粒厂家价格。

我公司正在优惠促销克隆与表达等系列产品，期待您的咨询选购N端Myc标签融合蛋白质粒厂家价格N端Myc标签融合蛋白质粒厂家价格N端Myc标签融合蛋白质粒厂家价格。