本试剂盒只能用于科学研究，不得用于医学诊断本试剂盒只能用于科学研究，不得用于医学诊断本试剂盒只能用于科学研究，不得用于医学诊断本试剂盒只能用于科学研究，不得用于医学诊断本试剂盒只能用于科学研究，不得用于医学诊断本试剂盒只能用于科学研究，不得用于医学诊断本试剂盒只能用于科学研究，不得用于医学诊断本试剂盒只能用于科学研究，不得用于医学诊断本试剂盒只能用于科学研究，不得用于医学诊断大鼠（Rat）血管内皮细胞生长因子（VEGF）大鼠（Rat）血管内皮细胞生长因子（VEGF）大鼠（Rat）血管内皮细胞生长因子（VEGF）大鼠（大鼠（大鼠（大鼠（大鼠（大鼠（大鼠（RatRatRatRatRatRatRat）））））））血管内皮细胞生长因子（VEGF）血管内皮细胞生长因子（VEGF）血管内皮细胞生长因子（VEGF）血管内皮细胞生长因子（VEGF）血管内皮细胞生长因子（VEGF）血管内皮细胞生长因子（血管内皮细胞生长因子（VEGF）ELISA检测试剂盒ELISA检测试剂盒ELISA检测试剂盒ELISA检测试剂盒ELISA检测试剂盒ELISA检测试剂盒ELISA检测试剂盒ELISA检测试剂盒ELISA检测试剂盒ELISA检测试剂盒
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检测原理检测原理检测原理检测原理检测原理检测原理检测原理检测原理检测原理检测原理检测原理检测原理
试剂盒采用双抗体一步夹心法酶联免疫吸附试验（ELISA）。往预先包被血管内皮细胞生长因子（VEGF）抗体的包被微孔中，依次加入标本、标准品、HRP标记的检测抗体，经过温育并彻底洗涤。用底物TMB显色，TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的血管内皮细胞生长因子（VEGF）呈正相关。用酶标仪在450nm 波长下测定吸光度（OD 值），计算样品浓度。试剂盒采用双抗体一步夹心法酶联免疫吸附试验（ELISA）。往预先包被血管内皮细胞生长因子（VEGF）抗体的包被微孔中，依次加入标本、标准品、HRP标记的检测抗体，经过温育并彻底洗涤。用底物TMB显色，TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的血管内皮细胞生长因子（VEGF）呈正相关。用酶标仪在450nm 波长下测定吸光度（OD 值），计算样品浓度。试剂盒采用双抗体一步夹心法酶联免疫吸附试验（ELISA）。往预先包被血管内皮细胞生长因子（VEGF）抗体的包被微孔中，依次加入标本、标准品、HRP标记的检测抗体，经过温育并彻底洗涤。用底物TMB显色，TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的血管内皮细胞生长因子（VEGF）呈正相关。用酶标仪在450nm 波长下测定吸光度（OD 值），计算样品浓度。试剂盒采用双抗体一步夹心法酶联免疫吸附试验（ELISA）。往预先包被血管内皮细胞生长因子（VEGF）抗体的包被微孔中，依次加入标本、标准品、HRP标记的检测抗体，经过温育并彻底洗涤。用底物TMB显色，TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的血管内皮细胞生长因子（VEGF）呈正相关。用酶标仪在450nm 波长下测定吸光度（OD 值），计算样品浓度。试剂盒采用双抗体一步夹心法酶联免疫吸附试验（ELISA）。试剂盒采用双抗体一步夹心法酶联免疫吸附试验（ELISA）。试剂盒采用双抗体一步夹心法酶联免疫吸附试验（ELISA）。试剂盒采用双抗体一步夹心法酶联免疫吸附试验（试剂盒采用双抗体一步夹心法酶联免疫吸附试验（ELISA）。往预先包往预先包往预先包往预先包往预先包被被被被被血管内皮细胞生长因子（VEGF）血管内皮细胞生长因子（VEGF）血管内皮细胞生长因子（VEGF）血管内皮细胞生长因子（血管内皮细胞生长因子（VEGF）抗体的包被微孔中，依次加入标本、标准品、HRP标记的检测抗体，经过温育并彻底洗涤。用底物TMB显色，TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的抗体的包被微孔中，依次加入标本、标准品、HRP标记的检测抗体，经过温育并彻底洗涤。用底物TMB显色，TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的抗体的包被微孔中，依次加入标本、标准品、HRP标记的检测抗体，经过温育并彻底洗涤。用底物TMB显色，TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的抗体的包被微孔中，依次加入标本、标准品、抗体的包被微孔中，依次加入标本、标准品、HRP标记的检测抗体，经过温育并彻底洗涤。用底物TMB显色，TMB在过氧化物酶的催化下转化成蓝色，并在酸的作用下转化成最终的黄色。颜色的深浅和样品中的血管内皮细胞生长因子（VEGF）血管内皮细胞生长因子（VEGF）血管内皮细胞生长因子（VEGF）血管内皮细胞生长因子（血管内皮细胞生长因子（VEGF）呈呈呈呈呈正相关。用酶标仪在450nm 波长下测定吸光度（OD 值），计算样品浓度。正相关。用酶标仪在450nm 波长下测定吸光度（OD 值），计算样品浓度。正相关。用酶标仪在450nm 波长下测定吸光度（OD 值），计算样品浓度。正相关。用酶标仪在正相关。用酶标仪在450nm 波长下测定吸光度（OD 值），计算样品浓度。
样品收集、处理及保存方法样品收集、处理及保存方法样品收集、处理及保存方法样品收集、处理及保存方法样品收集、处理及保存方法样品收集、处理及保存方法样品收集、处理及保存方法样品收集、处理及保存方法样品收集、处理及保存方法样品收集、处理及保存方法样品收集、处理及保存方法样品收集、处理及保存方法
1. 血清：使用不含热原和内毒素的试管，操作过程中避免任何细胞刺激，收集血液后，3000转离心10分钟将血清和红细胞迅速小心地分离。1. 血清：使用不含热原和内毒素的试管，操作过程中避免任何细胞刺激，收集血液后，3000转离心10分钟将血清和红细胞迅速小心地分离。1. 血清：使用不含热原和内毒素的试管，操作过程中避免任何细胞刺激，收集血液后，3000转离心10分钟将血清和红细胞迅速小心地分离。1. 血清：使用不含热原和内毒素的试管，操作过程中避免任何细胞刺激，收集血液后，3000转离心10分钟将血清和红细胞迅速小心地分离。1. 血清：使用不含热原和内毒素的试管，操作过程中避免任何细胞刺激，收集血液后，3000转离心10分钟将血清和红细胞迅速小心地分离。1. 血清：使用不含热原和内毒素的试管，操作过程中避免任何细胞刺激，收集血液后，3000转离心10分钟将血清和红细胞迅速小心地分离。1. 血清：使用不含热原和内毒素的试管，操作过程中避免任何细胞刺激，收集血液后，3000转离心10分钟将血清和红细胞迅速小心地分离。1. 血清：使用不含热原和内毒素的试管，操作过程中避免任何细胞刺激，收集血液后，3000转离心10分钟将血清和红细胞迅速小心地分离。
2. 血浆：EDTA、柠檬酸盐或肝素抗凝。3000转离心30分钟取上清。2. 血浆：EDTA、柠檬酸盐或肝素抗凝。3000转离心30分钟取上清。2. 血浆：EDTA、柠檬酸盐或肝素抗凝。3000转离心30分钟取上清。2. 血浆：EDTA、柠檬酸盐或肝素抗凝。3000转离心30分钟取上清。2. 血浆：EDTA、柠檬酸盐或肝素抗凝。3000转离心30分钟取上清。2. 血浆：EDTA、柠檬酸盐或肝素抗凝。3000转离心30分钟取上清。2. 血浆：EDTA、柠檬酸盐或肝素抗凝。3000转离心30分钟取上清。2. 血浆：EDTA、柠檬酸盐或肝素抗凝。3000转离心30分钟取上清。
3. 细胞上清液：3000转离心10分钟去除颗粒和聚合物。3. 细胞上清液：3000转离心10分钟去除颗粒和聚合物。3. 细胞上清液：3000转离心10分钟去除颗粒和聚合物。3. 细胞上清液：3000转离心10分钟去除颗粒和聚合物。3. 细胞上清液：3000转离心10分钟去除颗粒和聚合物。3. 细胞上清液：3000转离心10分钟去除颗粒和聚合物。3. 细胞上清液：3000转离心10分钟去除颗粒和聚合物。3. 细胞上清液：3000转离心10分钟去除颗粒和聚合物。
4. 组织匀浆：将组织加入适量生理盐水捣碎。3000转离心10分钟取上清。4. 组织匀浆：将组织加入适量生理盐水捣碎。3000转离心10分钟取上清。4. 组织匀浆：将组织加入适量生理盐水捣碎。3000转离心10分钟取上清。4. 组织匀浆：将组织加入适量生理盐水捣碎。3000转离心10分钟取上清。4. 组织匀浆：将组织加入适量生理盐水捣碎。3000转离心10分钟取上清。4. 组织匀浆：将组织加入适量生理盐水捣碎。3000转离心10分钟取上清。4. 组织匀浆：将组织加入适量生理盐水捣碎。3000转离心10分钟取上清。4. 组织匀浆：将组织加入适量生理盐水捣碎。3000转离心10分钟取上清。
5. 保存：如果样本收集后不及时检测，请按一次用量分装，冻存于-20℃，避免反复冻融，在室温下解冻并确保样品均匀地充分解冻。5. 保存：如果样本收集后不及时检测，请按一次用量分装，冻存于-20℃，避免反复冻融，在室温下解冻并确保样品均匀地充分解冻。5. 保存：如果样本收集后不及时检测，请按一次用量分装，冻存于-20℃，避免反复冻融，在室温下解冻并确保样品均匀地充分解冻。5. 保存：如果样本收集后不及时检测，请按一次用量分装，冻存于-20℃，避免反复冻融，在室温下解冻并确保样品均匀地充分解冻。5. 保存：如果样本收集后不及时检测，请按一次用量分装，冻存于-20℃，避免反复冻融，在室温下解冻并确保样品均匀地充分解冻。5. 保存：如果样本收集后不及时检测，请按一次用量分装，冻存于-20℃，避免反复冻融，在室温下解冻并确保样品均匀地充分解冻。5. 保存：如果样本收集后不及时检测，请按一次用量分装，冻存于-20℃，避免反复冻融，在室温下解冻并确保样品均匀地充分解冻。5. 保存：如果样本收集后不及时检测，请按一次用量分装，冻存于-20℃，避免反复冻融，在室温下解冻并确保样品均匀地充分解冻。
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1. 酶标仪（450nm）
2. 高精度加样器及枪头：0.5-10uL、2-20uL、20-200uL、200-1000uL
3. 37℃恒温箱

酶标仪（450nm）

酶标仪（450nm）酶标仪（450nm）酶标仪（450nm）酶标仪（450nm）酶标仪（450nm）酶标仪（450nm）酶标仪（酶标仪（450nm）

高精度加样器及枪头：0.5-10uL、2-20uL、20-200uL、200-1000uL

高精度加样器及枪头：0.5-10uL、2-20uL、20-200uL、200-1000uL高精度加样器及枪头：0.5-10uL、2-20uL、20-200uL、200-1000uL高精度加样器及枪头：0.5-10uL、2-20uL、20-200uL、200-1000uL高精度加样器及枪头：0.5-10uL、2-20uL、20-200uL、200-1000uL高精度加样器及枪头：0.5-10uL、2-20uL、20-200uL、200-1000uL高精度加样器及枪头：0.5-10uL、2-20uL、20-200uL、200-1000uL高精度加样器及枪头：高精度加样器及枪头：0.5-10uL、2-20uL、20-200uL、200-1000uL

37℃恒温箱

37℃恒温箱37℃恒温箱37℃恒温箱37℃恒温箱37℃恒温箱37℃恒温箱37℃恒温箱37℃恒温箱操作注意事项操作注意事项操作注意事项操作注意事项操作注意事项操作注意事项操作注意事项操作注意事项操作注意事项操作注意事项操作注意事项操作注意事项

1. 试剂盒保存在2-8℃，使用前室温平衡20分钟。从冰箱取出的浓缩洗涤液会有结晶，这属于正常现象，水浴加热使结晶完全溶解后再使用。
2. 实验中不用的板条应立即放回自封袋中，密封（低温干燥）保存。
3. 浓度为0的S0号标准品即可视为阴性对照或者空白；按照说明书操作时样本已经稀释5倍，最终结果乘以5才是样本实际浓度。
4. 严格按照说明书中标明的时间、加液量及顺序进行温育操作。
5. 所有液体组分使用前充分摇匀。

试剂盒保存在2-8℃，使用前室温平衡20分钟。从冰箱取出的浓缩洗涤液会有结晶，这属于正常现象，水浴加热使结晶完全溶解后再使用。

试剂盒保存在2-8℃，使用前室温平衡20分钟。从冰箱取出的浓缩洗涤液会有结晶，这属于正常现象，水浴加热使结晶完全溶解后再使用。试剂盒保存在2-8℃，使用前室温平衡20分钟。从冰箱取出的浓缩洗涤液会有结晶，这属于正常现象，水浴加热使结晶完全溶解后再使用。试剂盒保存在2-8℃，使用前室温平衡20分钟。从冰箱取出的浓缩洗涤液会有结晶，这属于正常现象，水浴加热使结晶完全溶解后再使用。试剂盒保存在2-8℃，使用前室温平衡20分钟。从冰箱取出的浓缩洗涤液会有结晶，这属于正常现象，水浴加热使结晶完全溶解后再使用。试剂盒保存在2-8℃，使用前室温平衡20分钟。从冰箱取出的浓缩洗涤液会有结晶，这属于正常现象，水浴加热使结晶完全溶解后再使用。试剂盒保存在2-8℃，使用前室温平衡20分钟。从冰箱取出的浓缩洗涤液会有结晶，这属于正常现象，水浴加热使结晶完全溶解后再使用。试剂盒保存在试剂盒保存在2-8℃，使用前室温平衡20分钟。从冰箱取出的浓缩洗涤液会有结晶，这属于正常现象，水浴加热使结晶完全溶解后再使用。

实验中不用的板条应立即放回自封袋中，密封（低温干燥）保存。

实验中不用的板条应立即放回自封袋中，密封（低温干燥）保存。实验中不用的板条应立即放回自封袋中，密封（低温干燥）保存。实验中不用的板条应立即放回自封袋中，密封（低温干燥）保存。实验中不用的板条应立即放回自封袋中，密封（低温干燥）保存。实验中不用的板条应立即放回自封袋中，密封（低温干燥）保存。实验中不用的板条应立即放回自封袋中，密封（低温干燥）保存。实验中不用的板条应立即放回自封袋中，密封（低温干燥）保存。实验中不用的板条应立即放回自封袋中，密封（低温干燥）保存。

浓度为0的S0号标准品即可视为阴性对照或者空白；按照说明书操作时样本已经稀释5倍，最终结果乘以5才是样本实际浓度。

浓度为0的S0号标准品即可视为阴性对照或者空白；按照说明书操作时样本已经稀释5倍，最终结果乘以5才是样本实际浓度。浓度为0的S0号标准品即可视为阴性对照或者空白；按照说明书操作时样本已经稀释5倍，最终结果乘以5才是样本实际浓度。浓度为0的S0号标准品即可视为阴性对照或者空白；按照说明书操作时样本已经稀释5倍，最终结果乘以5才是样本实际浓度。浓度为0的S0号标准品即可视为阴性对照或者空白；浓度为0的S0号标准品即可视为阴性对照或者空白；浓度为0的S0号标准品即可视为阴性对照或者空白；浓度为浓度为0的S0号标准品即可视为阴性对照或者空白；按照说明书操作时样本已经稀释5倍，最终结果乘以5才是样本实际浓度按照说明书操作时样本已经稀释5倍，最终结果乘以5才是样本实际浓度按照说明书操作时样本已经稀释5倍，最终结果乘以5才是样本实际浓度按照说明书操作时样本已经稀释按照说明书操作时样本已经稀释5倍，最终结果乘以5才是样本实际浓度。。。。。

严格按照说明书中标明的时间、加液量及顺序进行温育操作。

严格按照说明书中标明的时间、加液量及顺序进行温育操作。严格按照说明书中标明的时间、加液量及顺序进行温育操作。严格按照说明书中标明的时间、加液量及顺序进行温育操作。严格按照说明书中标明的时间、加液量及顺序进行温育操作。严格按照说明书中标明的时间、加液量及顺序进行温育操作。严格按照说明书中标明的时间、加液量及顺序进行温育操作。严格按照说明书中标明的时间、加液量及顺序进行温育操作。严格按照说明书中标明的时间、加液量及顺序进行温育操作。

所有液体组分使用前充分摇匀。

所有液体组分使用前充分摇匀。所有液体组分使用前充分摇匀。所有液体组分使用前充分摇匀。所有液体组分使用前充分摇匀。所有液体组分使用前充分摇匀。所有液体组分使用前充分摇匀。所有液体组分使用前充分摇匀。所有液体组分使用前充分摇匀。试剂盒组成试剂盒组成试剂盒组成试剂盒组成试剂盒组成试剂盒组成试剂盒组成试剂盒组成试剂盒组成试剂盒组成试剂盒组成试剂盒组成

名称	96孔配置	48孔配置	备注
微孔酶标板	12孔×8条	12孔×4条	无
标准品	0.3mL*6管	0.3mL*6管	无
样本稀释液	6mL	3mL	无
检测抗体-HRP	10mL	5mL	无
20×洗涤缓冲液	25mL	15mL	按说明书进行稀释
底物A	6mL	3mL	无
底物B	6mL	3mL	无
终止液	6mL	3mL	无
封板膜	2张	2张	无
说明书	1份	1份	无
自封袋	1个	1个	无

名称96孔配置48孔配置备注微孔酶标板12孔×8条12孔×4条无标准品0.3mL*6管0.3mL*6管无样本稀释液6mL3mL无检测抗体-HRP10mL5mL无20×洗涤缓冲液25mL15mL按说明书进行稀释底物A6mL3mL无底物B6mL3mL无终止液6mL3mL无封板膜2张2张无说明书1份1份无自封袋1个1个无名称96孔配置48孔配置备注名称名称名称名称名称名称名称名称96孔配置96孔配置96孔配置96孔配置96孔配置96孔配置96孔配置96孔配置48孔配置48孔配置48孔配置48孔配置48孔配置48孔配置48孔配置48孔配置备注备注备注备注备注备注备注备注微孔酶标板12孔×8条12孔×4条无微孔酶标板微孔酶标板微孔酶标板微孔酶标板微孔酶标板微孔酶标板微孔酶标板12孔×8条12孔×8条12孔×8条12孔×8条12孔×8条12孔×8条12孔×8条12孔×4条12孔×4条12孔×4条12孔×4条12孔×4条12孔×4条12孔×4条无无无无无无无标准品0.3mL*6管0.3mL*6管无标准品标准品标准品标准品标准品标准品标准品0.3mL*6管0.3mL*6管0.3mL*6管0.3mL*6管0.3mL*6管0.3mL*6管0.3mL*6管0.3mL*6管0.3mL*6管0.3mL*6管0.3mL*6管0.3mL*6管0.3mL*6管0.3mL*6管无无无无无无无样本稀释液6mL3mL无样本稀释液样本稀释液样本稀释液样本稀释液样本稀释液样本稀释液样本稀释液6mL6mL6mL6mL6mL6mL6mL3mL3mL3mL3mL3mL3mL3mL无无无无无无无检测抗体-HRP10mL5mL无检测抗体-HRP检测抗体-HRP检测抗体-HRP检测抗体-HRP检测抗体-HRP检测抗体检测抗体-HRP10mL10mL10mL10mL10mL10mL10mL5mL5mL5mL5mL5mL5mL5mL无无无无无无无20×洗涤缓冲液25mL15mL按说明书进行稀释20×洗涤缓冲液20×洗涤缓冲液20×洗涤缓冲液20×洗涤缓冲液20×洗涤缓冲液20×洗涤缓冲液20×洗涤缓冲液25mL25mL25mL25mL25mL25mL25mL15mL15mL15mL15mL15mL15mL15mL按说明书进行稀释按说明书进行稀释按说明书进行稀释按说明书进行稀释按说明书进行稀释按说明书进行稀释按说明书进行稀释底物A6mL3mL无底物A底物A底物A底物A底物A底物底物A6mL6mL6mL6mL6mL6mL6mL3mL3mL3mL3mL3mL3mL3mL无无无无无无无底物B6mL3mL无底物B底物B底物B底物B底物B底物底物B6mL6mL6mL6mL6mL6mL6mL3mL3mL3mL3mL3mL3mL3mL无无无无无无无终止液6mL3mL无终止液终止液终止液终止液终止液终止液终止液6mL6mL6mL6mL6mL6mL6mL3mL3mL3mL3mL3mL3mL3mL无无无无无无无封板膜2张2张无封板膜封板膜封板膜封板膜封板膜封板膜封板膜2张2张2张2张2张2张2张2张2张2张2张2张2张2张无无无无无无无说明书1份1份无说明书说明书说明书说明书说明书说明书说明书1份1份1份1份1份1份1份1份1份1份1份1份1份1份无无无无无无无自封袋1个1个无自封袋自封袋自封袋自封袋自封袋自封袋自封袋1个1个1个1个1个1个1个1个1个1个1个1个1个1个无无无无无无无 注：标准品（S0-S5）浓度依次为：0、20、40、80、160、320 pg/mL注：标准品（S0-S5）浓度依次为：0、20、40、80、160、320 pg/mL注：标准品（S0-S5）浓度注：标准品（S0-S5）浓度注：标准品（注：标准品（S0-S5）浓度依次依次依次依次为：为：为：为：为：0、20、40、80、160、320 p0、20、40、80、160、320 p0、20、40、80、160、320 p0、20、40、80、160、320 p0、20、40、80、160、320 pg/mLg/mLg/mLg/mLg/mL
试剂的准备试剂的准备试剂的准备试剂的准备试剂的准备试剂的准备试剂的准备试剂的准备试剂的准备试剂的准备试剂的准备试剂的准备
20×洗涤缓冲液的稀释：蒸馏水按1：20稀释，即1份的20×洗涤缓冲液加19份的蒸馏水。20×洗涤缓冲液的稀释：蒸馏水按1：20稀释，即1份的20×洗涤缓冲液加19份的蒸馏水。20×洗涤缓冲液的稀释：蒸馏水按1：20稀释，即1份的20×洗涤缓冲液加19份的蒸馏水。20×洗涤缓冲液的稀释：蒸馏水按1：20稀释，即1份的20×洗涤缓冲液加19份的蒸馏水。20×洗涤缓冲液的稀释：蒸馏水按1：20稀释，即1份的20×洗涤缓冲液加19份的蒸馏水。20×洗涤缓冲液的稀释：蒸馏水按1：20稀释，即1份的20×洗涤缓冲液加19份的蒸馏水。20×洗涤缓冲液的稀释：蒸馏水按1：20稀释，即1份的20×洗涤缓冲液加19份的蒸馏水。20×洗涤缓冲液的稀释：蒸馏水按1：20稀释，即1份的20×洗涤缓冲液加19份的蒸馏水。
洗板方法洗板方法洗板方法洗板方法洗板方法洗板方法洗板方法洗板方法洗板方法洗板方法洗板方法洗板方法

1. 手工洗板：甩尽孔内液体，每孔加满洗涤液，静置1min后甩尽孔内液体，在吸水纸上拍干，如此洗板5次。
2. 自动洗板机：每孔注入洗液350μL，浸泡1min，洗板5次。

手工洗板：甩尽孔内液体，每孔加满洗涤液，静置1min后甩尽孔内液体，在吸水纸上拍干，如此洗板5次。

手工洗板：甩尽孔内液体，每孔加满洗涤液，静置1min后甩尽孔内液体，在吸水纸上拍干，如此洗板5次。手工洗板：甩尽孔内液体，每孔加满洗涤液，静置1min后甩尽孔内液体，在吸水纸上拍干，如此洗板5次。手工洗板：甩尽孔内液体，每孔加满洗涤液，静置1min后甩尽孔内液体，在吸水纸上拍干，如此洗板5次。手工洗板：甩尽孔内液体，每孔加满洗涤液，静置1min后甩尽孔内液体，在吸水纸上拍干，如此洗板5次。手工洗板：甩尽孔内液体，每孔加满洗涤液，静置1min后甩尽孔内液体，在吸水纸上拍干，如此洗板5次。手工洗板：甩尽孔内液体，每孔加满洗涤液，静置1min后甩尽孔内液体，在吸水纸上拍干，如此洗板5次。手工洗板：甩尽孔内液体，每孔加满洗涤液，静置手工洗板：甩尽孔内液体，每孔加满洗涤液，静置1min后甩尽孔内液体，在吸水纸上拍干，如此洗板5次。

自动洗板机：每孔注入洗液350μL，浸泡1min，洗板5次。

自动洗板机：每孔注入洗液350μL，浸泡1min，洗板5次。自动洗板机：每孔注入洗液350μL，浸泡1min，洗板5次。自动洗板机：每孔注入洗液350μL，浸泡1min，洗板5次。自动洗板机：每孔注入洗液350μL，浸泡1min，洗板5次。自动洗板机：每孔注入洗液350μL，浸泡1min，洗板5次。自动洗板机：每孔注入洗液350μL，浸泡1min，洗板5次。自动洗板机：每孔注入洗液自动洗板机：每孔注入洗液350μL，浸泡1min，洗板5次。操作步骤操作步骤操作步骤操作步骤操作步骤操作步骤操作步骤操作步骤操作步骤操作步骤操作步骤操作步骤

1. 从室温平衡20min后的铝箔袋中取出所需板条，剩余板条用自封袋密封放回4℃。
2. 设置标准品孔和样本孔，标准品孔各加不同浓度的标准品50μL；
3. 样本孔先加待测样本10μL，再加样本稀释液40μL；空白孔不加。
4. 除空白孔外，标准品孔和样本孔中每孔加入辣根过氧化物酶（HRP）标记的检测抗体100μL，用封板膜封住反应孔，37℃水浴锅或恒温箱温育60min。
5. 弃去液体，吸水纸上拍干，每孔加满洗涤液，静置1min，甩去洗涤液，吸水纸上拍干，如此重复洗板5次（也可用洗板机洗板）。
6. 每孔加入底物A、B各50μL，37℃避光孵育15min。
7. 每孔加入终止液50μL，15min内，在450nm波长处测定各孔的OD值。

从室温平衡20min后的铝箔袋中取出所需板条，剩余板条用自封袋密封放回4℃。

从室温平衡20min后的铝箔袋中取出所需板条，剩余板条用自封袋密封放回4℃。从室温平衡20min后的铝箔袋中取出所需板条，剩余板条用自封袋密封放回4℃。从室温平衡20min后的铝箔袋中取出所需板条，剩余板条用自封袋密封放回4℃。从室温平衡20min后的铝箔袋中取出所需板条，剩余板条用自封袋密封放回4℃。从室温平衡20min后的铝箔袋中取出所需板条，剩余板条用自封袋密封放回4℃。从室温平衡20min后的铝箔袋中取出所需板条，剩余板条用自封袋密封放回4℃。从室温平衡从室温平衡20min后的铝箔袋中取出所需板条，剩余板条用自封袋密封放回4℃。

设置标准品孔和样本孔，标准品孔各加不同浓度的标准品50μL；

设置标准品孔和样本孔，标准品孔各加不同浓度的标准品50μL；设置标准品孔和样本孔，标准品孔各加不同浓度的标准品50μL；设置标准品孔和样本孔，标准品孔各加不同浓度的标准品50μL；设置标准品孔和样本孔，标准品孔各加不同浓度的标准品50μL；设置标准品孔和样本孔，标准品孔各加不同浓度的标准品50μL；设置标准品孔和样本孔，标准品孔各加不同浓度的标准品50μL；设置标准品孔和样本孔，标准品孔各加不同浓度的标准品设置标准品孔和样本孔，标准品孔各加不同浓度的标准品50μL；

样本孔先加待测样本10μL，再加样本稀释液40μL；空白孔不加。

样本孔先加待测样本10μL，再加样本稀释液40μL；空白孔不加。样本孔先加待测样本10μL，再加样本稀释液40μL；空白孔不加。样本孔先加待测样本10μL，再加样本稀释液40μL；空白孔不加。样本孔先加待测样本10μL，再加样本稀释液40μL；空白孔不加。样本孔先加待测样本10μL，再加样本稀释液40μL；空白孔不加。样本孔先加待测样本10μL，再加样本稀释液40μL；空白孔不加。样本孔先加待测样本样本孔先加待测样本10μL，再加样本稀释液40μL；空白孔不加。

除空白孔外，标准品孔和样本孔中每孔加入辣根过氧化物酶（HRP）标记的检测抗体100μL，用封板膜封住反应孔，37℃水浴锅或恒温箱温育60min。

除空白孔外，标准品孔和样本孔中每孔加入辣根过氧化物酶（HRP）标记的检测抗体100μL，用封板膜封住反应孔，37℃水浴锅或恒温箱温育60min。除空白孔外，标准品孔和样本孔中每孔加入辣根过氧化物酶（HRP）标记的检测抗体100μL，用封板膜封住反应孔，37℃水浴锅或恒温箱温育60min。除空白孔外，标准品孔和样本孔中每孔加入辣根过氧化物酶（HRP）标记的检测抗体100μL，用封板膜封住反应孔，37℃水浴锅或恒温箱温育60min。除空白孔外，标准品孔和样本孔中每孔加入辣根过氧化物酶（HRP）标记的检测抗体100μL，用封板膜封住反应孔，37℃水浴锅或恒温箱温育60min。除空白孔外，标准品孔和样本孔中每孔加入辣根过氧化物酶（HRP）标记的检测抗体100μL，用封板膜封住反应孔，37℃水浴锅或恒温箱温育60min。除空白孔外，标准品孔和样本孔中每孔加入辣根过氧化物酶（HRP）标记的检测抗体100μL，用封板膜封住反应孔，37℃水浴锅或恒温箱温育60min。除空白孔外，标准品孔和样本孔中每孔加入辣根过氧化物酶（除空白孔外，标准品孔和样本孔中每孔加入辣根过氧化物酶（HRP）标记的检测抗体100μL，用封板膜封住反应孔，37℃水浴锅或恒温箱温育60min。

弃去液体，吸水纸上拍干，每孔加满洗涤液，静置1min，甩去洗涤液，吸水纸上拍干，如此重复洗板5次（也可用洗板机洗板）。

弃去液体，吸水纸上拍干，每孔加满洗涤液，静置1min，甩去洗涤液，吸水纸上拍干，如此重复洗板5次（也可用洗板机洗板）。弃去液体，吸水纸上拍干，每孔加满洗涤液，静置1min，甩去洗涤液，吸水纸上拍干，如此重复洗板5次（也可用洗板机洗板）。弃去液体，吸水纸上拍干，每孔加满洗涤液，静置1min，甩去洗涤液，吸水纸上拍干，如此重复洗板5次（也可用洗板机洗板）。弃去液体，吸水纸上拍干，每孔加满洗涤液，静置1min，甩去洗涤液，吸水纸上拍干，如此重复洗板5次（也可用洗板机洗板）。弃去液体，吸水纸上拍干，每孔加满洗涤液，静置1min，甩去洗涤液，吸水纸上拍干，如此重复洗板5次（也可用洗板机洗板）。弃去液体，吸水纸上拍干，每孔加满洗涤液，静置1min，甩去洗涤液，吸水纸上拍干，如此重复洗板5次（也可用洗板机洗板）。弃去液体，吸水纸上拍干，每孔加满洗涤液，静置弃去液体，吸水纸上拍干，每孔加满洗涤液，静置1min，甩去洗涤液，吸水纸上拍干，如此重复洗板5次（也可用洗板机洗板）。

每孔加入底物A、B各50μL，37℃避光孵育15min。

每孔加入底物A、B各50μL，37℃避光孵育15min。每孔加入底物A、B各50μL，37℃避光孵育15min。每孔加入底物A、B各50μL，37℃避光孵育15min。每孔加入底物A、B各50μL，37℃避光孵育15min。每孔加入底物A、B各50μL，37℃避光孵育15min。每孔加入底物A、B各50μL，37℃避光孵育15min。每孔加入底物每孔加入底物A、B各50μL，37℃避光孵育15min。

每孔加入终止液50μL，15min内，在450nm波长处测定各孔的OD值。

每孔加入终止液50μL，15min内，在450nm波长处测定各孔的OD值。每孔加入终止液50μL，15min内，在450nm波长处测定各孔的OD值。每孔加入终止液50μL，15min内，在450nm波长处测定各孔的OD值。每孔加入终止液50μL，15min内，在450nm波长处测定各孔的OD值。每孔加入终止液50μL，15min内，在450nm波长处测定各孔的OD值。每孔加入终止液50μL，15min内，在450nm波长处测定各孔的OD值。每孔加入终止液每孔加入终止液50μL，15min内，在450nm波长处测定各孔的OD值。结果判断结果判断结果判断结果判断结果判断结果判断结果判断结果判断结果判断结果判断结果判断结果判断
绘制标准曲线：在Excel工作表中，以标准品浓度作横坐标，对应OD值作纵坐标，绘制出标准品线性回归曲线，按曲线方程计算各样本浓度值。绘制标准曲线：在Excel工作表中，以标准品浓度作横坐标，对应OD值作纵坐标，绘制出标准品线性回归曲线，按曲线方程计算各样本浓度值。绘制标准曲线：在Excel工作表中，以标准品浓度作横坐标，对应OD值作纵坐标，绘制出标准品线性回归曲线，按曲线方程计算各样本浓度值。绘制标准曲线：在Excel工作表中，以标准品浓度作横坐标，对应OD值作纵坐标，绘制出标准品线性回归曲线，按曲线方程计算各样本浓度值。绘制标准曲线：在Excel工作表中，以标准品浓度作横坐标，对应OD值作纵坐标，绘制出标准品线性回归曲线，按曲线方程计算各样本浓度值。绘制标准曲线：在Excel工作表中，以标准品浓度作横坐标，对应OD值作纵坐标，绘制出标准品线性回归曲线，按曲线方程计算各样本浓度值。绘制标准曲线：在绘制标准曲线：在Excel工作表中，以标准品浓度作横坐标，对应OD值作纵坐标，绘制出标准品线性回归曲线，按曲线方程计算各样本浓度值。

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试剂盒性能试剂盒性能试剂盒性能试剂盒性能试剂盒性能试剂盒性能试剂盒性能试剂盒性能试剂盒性能试剂盒性能试剂盒性能试剂盒性能

1. 准确性：标准品线性回归与预期浓度相关系数R值，大于等于0.9900。
2. 灵敏度：最低检测浓度小于1.0 pg/mL。
3. 特异性：不与其它可溶性结构类似物交叉反应。
4. 重复性：板内、板间变异系数均小于15%。
5. 贮藏：2-8℃，避光防潮保存。
6. 有效期：6个月

准确性：标准品线性回归与预期浓度相关系数R值，大于等于0.9900。

准确性：标准品线性回归与预期浓度相关系数R值，大于等于0.9900。准确性：标准品线性回归与预期浓度相关系数R值，大于等于0.9900。准确性：标准品线性回归与预期浓度相关系数R值，大于等于0.9900。准确性：标准品线性回归与预期浓度相关系数R值，大于等于0.9900。准确性：标准品线性回归与预期浓度相关系数R值，大于等于0.9900。准确性：标准品线性回归与预期浓度相关系数R值，大于等于0.9900。准确性：标准品线性回归与预期浓度相关系数准确性：标准品线性回归与预期浓度相关系数R值，大于等于0.9900。

灵敏度：最低检测浓度小于1.0 pg/mL。

灵敏度：最低检测浓度小于1.0 pg/mL。灵敏度：最低检测浓度小于1.0 pg/mL。灵敏度：最低检测浓度小于1.0 pg/mL。灵敏度：最低检测浓度小于灵敏度：最低检测浓度小于灵敏度：最低检测浓度小于灵敏度：最低检测浓度小于灵敏度：最低检测浓度小于1.0 pg/mL1.0 pg/mL1.0 pg/mL1.0 pg/mL1.0 pg/mL。。。。。

特异性：不与其它可溶性结构类似物交叉反应。

特异性：不与其它可溶性结构类似物交叉反应。特异性：不与其它可溶性结构类似物交叉反应。特异性：不与其它可溶性结构类似物交叉反应。特异性：不与其它可溶性结构类似物交叉反应。特异性：不与其它可溶性结构类似物交叉反应。特异性：不与其它可溶性结构类似物交叉反应。特异性：不与其它可溶性结构类似物交叉反应。特异性：不与其它可溶性结构类似物交叉反应。

重复性：板内、板间变异系数均小于15%。

重复性：板内、板间变异系数均小于15%。重复性：板内、板间变异系数均小于15%。重复性：板内、板间变异系数均小于15%。重复性：板内、板间变异系数均小于15%。重复性：板内、板间变异系数均小于15%。重复性：板内、板间变异系数均小于15%。重复性：板内、板间变异系数均小于重复性：板内、板间变异系数均小于15%。

贮藏：2-8℃，避光防潮保存。

贮藏：2-8℃，避光防潮保存。贮藏：2-8℃，避光防潮保存。贮藏：2-8℃，避光防潮保存。贮藏：2-8℃，避光防潮保存。贮藏：2-8℃，避光防潮保存。贮藏：2-8℃，避光防潮保存。贮藏：贮藏：2-8℃，避光防潮保存。

有效期：6个月

有效期：6个月有效期：6个月有效期：6个月有效期：6个月有效期：6个月有效期：6个月有效期：有效期：6个月免责声明免责声明免责声明免责声明免责声明免责声明免责声明免责声明免责声明免责声明免责声明免责声明

1. 试剂盒仅供研究使用，不得用于临床实验或人体实验，否则所产生的一切后果，由实验者承担，本公司概不负责。
2. 严格按照说明书操作，实验者违反说明书操作，后果由实验者承担。

试剂盒仅供研究使用，不得用于临床实验或人体实验，否则所产生的一切后果，由实验者承担，本公司概不负责。

试剂盒仅供研究使用，不得用于临床实验或人体实验，否则所产生的一切后果，由实验者承担，本公司概不负责。试剂盒仅供研究使用，不得用于临床实验或人体实验，否则所产生的一切后果，由实验者承担，本公司概不负责。试剂盒仅供研究使用，不得用于临床实验或人体实验，否则所产生的一切后果，由实验者承担，本公司概不负责。试剂盒仅供研究使用，不得用于临床实验或试剂盒仅供研究使用，不得用于临床实验或试剂盒仅供研究使用，不得用于临床实验或试剂盒仅供研究使用，不得用于临床实验或试剂盒仅供研究使用，不得用于临床实验或人人人人人体实验，否则所产生的一切后果，由实验者承担，本公司概不负责。体实验，否则所产生的一切后果，由实验者承担，本公司概不负责。体实验，否则所产生的一切后果，由实验者承担，本公司概不负责。体实验，否则所产生的一切后果，由实验者承担，本公司概不负责。体实验，否则所产生的一切后果，由实验者承担，本公司概不负责。

严格按照说明书操作，实验者违反说明书操作，后果由实验者承担。

严格按照说明书操作，实验者违反说明书操作，后果由实验者承担。严格按照说明书操作，实验者违反说明书操作，后果由实验者承担。严格按照说明书操作，实验者违反说明书操作，后果由实验者承担。严格按照说明书操作，实验者违反说明书操作，后果由实验者承担。严格按照说明书操作，实验者违反说明书操作，后果由实验者承担。严格按照说明书操作，实验者违反说明书操作，后果由实验者承担。严格按照说明书操作，实验者违反说明书操作，后果由实验者承担。严格按照说明书操作，实验者违反说明书操作，后果由实验者承担。
■针对客户购买情况公司有不同的优惠折扣，欢迎咨询订购~
■郑重申明：本公司销售所有产品均为实物拍摄，如产品出现质量问题，本公司包一切售后！！！
■500元以上包邮，本公司提供正规发票，货到付款，可按科研实验要求定制产品！！！
■本公司已与很多高校中科院及研发单位合作并也获得了老师的认可和推荐！！！

滁州仕诺达生物科技有限公司（Chuzhou shinuoda Biological Technology Co., Ltd.）是一家以技术开发、转让、销售及咨询服务为一体的生物科技有限公司，公司主要研发销售生物试剂，为全球科研客户提供优质的产品和技术服务。 

公司主营产品包括：ELISA试剂盒、动物血制品、标准品、染色液、试剂、抑制剂、酶相关试剂以及仪器设备等 经销品牌：Thermo Fisher Scientific、CST、abcam、santa cruz、sigma、Corning、Axygen、NEB、Novus、BD、R&D、阿拉丁（未提及品牌，本公司也可以代买） 公司以“产品质量第一，服务至上”为企业宗旨，秉承友好共赢的合作理念，立志给科研界老师提供我们力所能及的帮助。如果您对我公司的产品及服务有兴趣，期待您给我们个机会在线留言或者来电咨询。■针对客户购买情况公司有不同的优惠折扣，欢迎咨询订购~
■郑重申明：本公司销售所有产品均为实物拍摄，如产品出现质量问题，本公司包一切售后！！！
■500元以上包邮，本公司提供正规发票，货到付款，可按科研实验要求定制产品！！！
■本公司已与很多高校中科院及研发单位合作并也获得了老师的认可和推荐！！！

滁州仕诺达生物科技有限公司（Chuzhou shinuoda Biological Technology Co., Ltd.）是一家以技术开发、转让、销售及咨询服务为一体的生物科技有限公司，公司主要研发销售生物试剂，为全球科研客户提供优质的产品和技术服务。 

公司主营产品包括：ELISA试剂盒、动物血制品、标准品、染色液、试剂、抑制剂、酶相关试剂以及仪器设备等 经销品牌：Thermo Fisher Scientific、CST、abcam、santa cruz、sigma、Corning、Axygen、NEB、Novus、BD、R&D、阿拉丁（未提及品牌，本公司也可以代买） 公司以“产品质量第一，服务至上”为企业宗旨，秉承友好共赢的合作理念，立志给科研界老师提供我们力所能及的帮助。如果您对我公司的产品及服务有兴趣，期待您给我们个机会在线留言或者来电咨询。






FOR RESEARCH USE ONLY.FOR RESEARCH USE ONLY.FOR RESEARCH USE ONLY.FOR RESEARCH USE ONLY.FOR RESEARCH USE ONLY.FOR RESEARCH USE ONLY.FOR RESEARCH USE ONLY.
NOT FOR USE IN DIAGNOSTIC PROCEDURES.NOT FOR USE IN DIAGNOSTIC PROCEDURES.NOT FOR USE IN DIAGNOSTIC PROCEDURES.NOT FOR USE IN DIAGNOSTIC PROCEDURESNOT FOR USE IN DIAGNOSTIC PROCEDURESNOT FOR USE IN DIAGNOSTIC PROCEDURESNOT FOR USE IN DIAGNOSTIC PROCEDURES.....
RatVascular Endothelial cell Growth Factor (VEGF) ELISA Kit
instructionRatVascular Endothelial cell Growth Factor (VEGF) ELISA Kit
instructionRatRatRatRatRatRatVascular Endothelial cell Growth Factor (VEGF)Vascular Endothelial cell Growth Factor (VEGF)Vascular Endothelial cell Growth Factor (VEGF)Vascular Endothelial cell Growth Factor (VEGF)Vascular Endothelial cell Growth Factor (VEGF)Vascular Endothelial cell Growth Factor (VEGF) ELISA Kit
instruction ELISA Kit
instruction ELISA Kit
instruction ELISA Kit
instruction ELISA Kit
instruction
Intended useIntended useIntended useIntended useIntended useIntended useIntended use
This VEGF ELISA kit is intended Laboratory for Research use only and is not for use in diagnostic or therapeutic procedures.The Stop Solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration of VEGF in the sample, this VEGF ELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versus VEGF concentration. The concentration of VEGF in the samples is then determined by comparing the O.D. of the samples to the standard curve.This VEGF ELISA kit is intended Laboratory for Research use only and is not for use in diagnostic or therapeutic procedures.The Stop Solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration of VEGF in the sample, this VEGF ELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versus VEGF concentration. The concentration of VEGF in the samples is then determined by comparing the O.D. of the samples to the standard curve.This VEGF ELISA kit is intended Laboratory for Research use only and is not for use in diagnostic or therapeutic procedures.The Stop Solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration of VEGF in the sample, this VEGF ELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versus VEGF concentration. The concentration of VEGF in the samples is then determined by comparing the O.D. of the samples to the standard curve.This VEGF ELISA kit is intended Laboratory for Research use only and is not for use in diagnostic or therapeutic procedures.The Stop Solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration of VEGF in the sample, this VEGF ELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versus VEGF concentration. The concentration of VEGF in the samples is then determined by comparing the O.D. of the samples to the standard curve.This This This This VEGFVEGFVEGFVEGFVEGF ELISA kit is intended Laboratory for Research use only and is not for use in diagnostic or therapeutic procedures.The Stop Solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration of  ELISA kit is intended Laboratory for Research use only and is not for use in diagnostic or therapeutic procedures.The Stop Solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration of  ELISA kit is intended Laboratory for Research use only and is not for use in diagnostic or therapeutic procedures.The Stop Solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration of  ELISA kit is intended Laboratory for Research use only and is not for use in diagnostic or therapeutic procedures.The Stop Solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration of VEGFVEGFVEGFVEGFVEGF in the sample, this  in the sample, this  in the sample, this  in the sample, this VEGFVEGFVEGFVEGFVEGF ELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versus  ELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versus  ELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versus  ELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versus VEGFVEGFVEGFVEGFVEGF concentration. The concentration of  concentration. The concentration of  concentration. The concentration of  concentration. The concentration of VEGFVEGFVEGFVEGFVEGF in the samples is then determined by comparing the O.D. of the samples to the standard curve. in the samples is then determined by comparing the O.D. of the samples to the standard curve. in the samples is then determined by comparing the O.D. of the samples to the standard curve. in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Samplecollection and storagesSamplecollection and storagesSSSSampleampleampleampleamplecollection and storagescollection and storagescollection and storagescollection and storagescollection and storages
Serum - Use a serum separator tube and allow samples to clot for 30 minutes before centrifugation for 10 minutes at approximately 3000×g. Remove serum and assay immediately or aliquot and store samples at -20℃ or -80℃.Avoid repeated freeze-thaw cyclesSerum - Use a serum separator tube and allow samples to clot for 30 minutes before centrifugation for 10 minutes at approximately 3000×g. Remove serum and assay immediately or aliquot and store samples at -20℃ or -80℃.Avoid repeated freeze-thaw cyclesSerum - Use a serum separator tube and allow samples to clot for 30 minutes before centrifugation for 10 minutes at approximately 3000×g. Remove serum and assay immediately or aliquot and store samples at -20℃ or -80℃.Avoid repeated freeze-thaw cyclesSerumSerumSerumSerumSerum - Use a serum separator tube and allow samples to clot for 30 minutes before centrifugation for  - Use a serum separator tube and allow samples to clot for 30 minutes before centrifugation for  - Use a serum separator tube and allow samples to clot for 30 minutes before centrifugation for 11110 minutes at approximately 0 minutes at approximately 0 minutes at approximately 3333000×g. Remove serum and assay immediately or aliquot and store samples at -20℃ or -80℃.Avoid repeated freeze-thaw cycles000×g. Remove serum and assay immediately or aliquot and store samples at -20℃ or -80℃.Avoid repeated freeze-thaw cycles000×g. Remove serum and assay immediately or aliquot and store samples at -20℃ or -80℃.Avoid repeated freeze-thaw cycles
Plasma- Collect plasma using EDTA or heparin as an anticoagulant. Centrifuge samples for 30 minutes at 3000×g at 2-8℃ within 30 minutes of collection. Store samples at -20℃or -80℃. Avoid repeated freeze-thaw cycles.Plasma- Collect plasma using EDTA or heparin as an anticoagulant. Centrifuge samples for 30 minutes at 3000×g at 2-8℃ within 30 minutes of collection. Store samples at -20℃or -80℃. Avoid repeated freeze-thaw cycles.Plasma- Collect plasma using EDTA or heparin as an anticoagulant. Centrifuge samples for 30 minutes at 3000×g at 2-8℃ within 30 minutes of collection. Store samples at -20℃or -80℃. Avoid repeated freeze-thaw cycles.PlasmaPlasmaPlasmaPlasmaPlasma - Collect plasma using EDTA or heparin as an anticoagulant. Centrifuge samples for - Collect plasma using EDTA or heparin as an anticoagulant. Centrifuge samples for - Collect plasma using EDTA or heparin as an anticoagulant. Centrifuge samples for 30303030 minutes at  minutes at  minutes at 3333000×g at 2-8℃ within 30 minutes of collection. Store samples at -20℃or -80℃. Avoid repeated freeze-thaw cycles.000×g at 2-8℃ within 30 minutes of collection. Store samples at -20℃or -80℃. Avoid repeated freeze-thaw cycles.000×g at 2-8℃ within 30 minutes of collection. Store samples at -20℃or -80℃. Avoid repeated freeze-thaw cycles.
Cell culture supernates and other biological fluids - Remove particulates by centrifugation and assay immediately or aliquot and store samples at -20℃or -80℃. Avoid repeated freeze-thaw cycles.Cell culture supernates and other biological fluids - Remove particulates by centrifugation and assay immediately or aliquot and store samples at -20℃or -80℃. Avoid repeated freeze-thaw cycles.Cell culture supernates and other biological fluids - Remove particulates by centrifugation and assay immediately or aliquot and store samples at -20℃or -80℃. Avoid repeated freeze-thaw cycles.Cell culture supernates and other biological fluidsCell culture supernates and other biological fluidsCell culture supernates and other biological fluidsCell culture supernates and other biological fluidsCell culture supernates and other biological fluids - - - - Remove particulates by centrifugation and assay immediately or aliquot and store samples at -20℃or -80℃. Avoid repeated freeze-thaw cycles. Remove particulates by centrifugation and assay immediately or aliquot and store samples at -20℃or -80℃. Avoid repeated freeze-thaw cycles. Remove particulates by centrifugation and assay immediately or aliquot and store samples at -20℃or -80℃. Avoid repeated freeze-thaw cycles.
Note: The samples shoule be centrifugated dequately and no hemolysis or granule was allowed.Note: The samples shoule be centrifugated dequately and no hemolysis or granule was allowed.Note: The samples shoule be centrifugated dequately and no hemolysis or granule was allowed.Note: Note: Note: Note: Note: Note: Note: The samples shoule be centrifugated dequately and no hemolysis or granule was allowed.The samples shoule be centrifugated dequately and no hemolysis or granule was allowed.The samples shoule be centrifugated dequately and no hemolysis or granule was allowed.The samples shoule be centrifugated dequately and no hemolysis or granule was allowed.
Materials required but not suppliedMaterials required but not suppliedMaterials required but not suppliedMaterials required but not suppliedMaterials required but not suppliedMaterials required but not supplied
1. Standard microplate reader(450nm)1. Standard microplate reader(450nm)1. Standard microplate reader(450nm)1. 1. 1. 1. Standard microplate readerStandard microplate readerStandard microplate reader(450nm)(450nm)(450nm)(450nm)
2. Precision pipettes and Disposable pipette tips.2. Precision pipettes and Disposable pipette tips.2. Precision pipettes and Disposable pipette tips.2. 2. 2. 2. Precision pipettes and Disposable pipette tipsPrecision pipettes and Disposable pipette tipsPrecision pipettes and Disposable pipette tips....
3. 37 ℃ incubator3. 37 ℃ incubator3. 37 ℃ incubator3. 3. 3. 3. 37 ℃ incubator37 ℃ incubator37 ℃ incubator
PrecautionsPrecautionsPrecautionsPPPPPPPrecautionsrecautionsrecautionsrecautionsrecautionsrecautionsrecautionsrecautions
1. Donot substitutereagentsfromone kit to another.Standard, conjugateandmicroplates are matchedfor optimal performance. Useonly thereagentssuppliedby manufacturer.1. Donot substitutereagentsfromone kit to another.Standard, conjugateandmicroplates are matchedfor optimal performance. Useonly thereagentssuppliedby manufacturer.1. Donot substitutereagentsfromone kit to another.Standard, conjugateandmicroplates are matchedfor optimal performance. Useonly thereagentssuppliedby manufacturer.1. 1. 1. 1. 1. DDDDDDooooo notnotnotnotnot s s s s s subububububstitstitstitstitstitstituuuuuutttttteeeee rrrrrreageeageeageeageeagennnnnnttttttsssss frfrfrfrfrfromomomomom one kone kone kone kone kiiiiiittttt t t t t t to anoo anoo anoo anoo anottttttheheheheherrrrrr..... SSSSSttttttandandandandandaaaaaarrrrrrd, cod, cod, cod, cod, connnnnnjjjjjjugugugugugaaaaaatttttteeeee andandandandand mmmmmmiiiiiicccccrrrrrropopopopopllllllaaaaaattttttes aes aes aes aes arrrrrre e e e e mmmmmmaaaaaattttttchedchedchedchedched ffffffor opor opor opor opor optitititititimmmmmmal peal peal peal peal perfrfrfrfrfrfooooorrrrrrmmmmmmance.ance.ance.ance.ance. U U U U U Usssssseeeee onononononlllllly y y y y tttttthehehehehe rrrrrreageeageeageeageeagennnnnnttttttsssss ssssssuppuppuppuppupplilililililiededededed by by by by by mmmmmmanuanuanuanuanuffffffacacacacacttttttuuuuurrrrrreeeeerrrrrr.....
2. Donot removemicroplatefrom the storage baguntilneeded. Unusedstripsshouldbe stored at2-8°Cin their pouchwith the desiccantprovided.2. Donot removemicroplatefrom the storage baguntilneeded. Unusedstripsshouldbe stored at2-8°Cin their pouchwith the desiccantprovided.2. Donot removemicroplatefrom the storage baguntilneeded. Unusedstripsshouldbe stored at2-8°Cin their pouchwith the desiccantprovided.2. 2. 2. 2. 2. DDDDDDooooo notnotnotnotnot r r r r r reeeeemmmmmmooooovvvvvveeeee mmmmmmiiiiiicccccrrrrrropopopopopllllllaaaaaatttttteeeee frfrfrfrfrfromomomomom t t t t t the he he he he ststststststooooorrrrrrage bagage bagage bagage bagage bag uuuuunnnnnnttttttiiiiiilllll needed.needed.needed.needed.needed. U U U U U Unununununussssssededededed stristristristristristripspspspsps sssssshouhouhouhouhoullllllddddd be be be be be ststststststooooorrrrrred ated ated ated ated at 22222------8°C8°C8°C8°C8°C iiiiiin n n n n ttttttheheheheheiiiiiir pouchr pouchr pouchr pouchr pouch witwitwitwitwitwithhhhh t t t t t the dehe dehe dehe dehe desisisisisisiccaccaccaccaccannnnnnttttt ppppprrrrrrooooovvvvvviiiiiided.ded.ded.ded.ded.
3. Mix all reagents before using.3. Mix all reagents before using.3. Mix all reagents before using.333333. . . . . Mix all reagents before using.Mix all reagents before using.Mix all reagents before using.Mix all reagents before using.Mix all reagents before using.Mix all reagents before using.
Remove allkit reagentsfrom refrigerator and allow them to reachroom temperature( 20-25°C)Remove allkit reagentsfrom refrigerator and allow them to reachroom temperature( 20-25°C)Remove allkit reagentsfrom refrigerator and allow them to reachroom temperature( 20-25°C)RRRRRReeeeemmmmmmooooovvvvvve ae ae ae ae alllllllllll kkkkkiiiiiittttt r r r r r reageeageeageeageeagennnnnnttttttsssss frfrfrfrfrfromomomomom r r r r r reeeeefrifrifrifrifrifrigegegegegerrrrrraaaaaattttttor and aor and aor and aor and aor and allllllllllllowowowowow t t t t t themhemhemhemhem t t t t t to o o o o rrrrrreacheacheacheacheach rrrrrroomoomoomoomoom t t t t t teeeeemmmmmmpepepepeperrrrrraaaaaattttttuuuuurrrrrreeeee ( 20( 20( 20( 20( 20------22222555555°°°°°CCCCCC))))))
Materials suppliedMaterials suppliedMaterials suppliedMaterials suppliedMaterials suppliedMaterials suppliedMaterials supplied

Name	96determinations	48determinations
Microelisa stripplate	12*8strips	12*4strips
Standard	0.3ml*6tubes	0.3ml*6tubes
Sample Diluent	6.0ml	3.0ml
HRP-Conjugate reagent	10.0ml	5.0ml
20X Wash solution	25ml	15ml
Chromogen Solution A	6.0ml	3.0ml
Chromogen Solution B	6.0ml	3.0ml
Stop Solution	6.0ml	3.0ml
Closure plate membrane	2	2
User manual	1	1
Sealed bags	1	1

Name96determinations48determinationsMicroelisa stripplate12*8strips12*4stripsStandard0.3ml*6tubes0.3ml*6tubesSample Diluent6.0ml3.0mlHRP-Conjugate reagent10.0ml5.0ml20X Wash solution25ml15mlChromogen Solution A6.0ml3.0mlChromogen Solution B6.0ml3.0mlStop Solution6.0ml3.0mlClosure plate membrane22User manual11Sealed bags11Name96determinations48determinationsNameNameNameNameNameNameName96determinations96determinations96determinations96969696 determinationsdeterminationsdeterminationsdeterminations48determinations48determinations48determinations48484848 determinationsdeterminationsdeterminationsdeterminationsMicroelisa stripplate12*8strips12*4stripsMicroelisa stripplateMicroelisa stripplateMicroelisa stripplateMicroelisa stripplateMicroelisa stripplateMicroelisa stripplateMicroelisa stripplate12*8strips12*8strips12*8strips12*8strips12*8strips12*8strips12*8strips12*4strips12*4strips12*4strips1112*4strips2*4strips2*4strips2*4stripsStandard0.3ml*6tubes0.3ml*6tubesStandardStandardStandardStandardStandardStandard0.3ml*6tubes0.3ml*6tubes0.3ml*6tubes0.0.0.3333mlmlml*6tubes*6tubes*6tubes*6tubes0.3ml*6tubes0.3ml*6tubes0.3ml*6tubes0.0.0.3333mlmlml*6tubes*6tubes*6tubes*6tubesSample Diluent6.0ml3.0mlSample DiluentSample DiluentSample DiluentSample DiluentSample DiluentSample DiluentSample DiluentSample Diluent6.0ml6.0ml6.0ml6.06.06.06.06.0mlmlmlml3.0ml3.0ml3.0ml3.0ml3.0ml3.0ml3.0ml3.0mlHRP-Conjugate reagent10.0ml5.0mlHRP-Conjugate reagentHRP-Conjugate reagentHRP-Conjugate reagentHRP-Conjugate reagentHRP-Conjugate reagentHRP-Conjugate reagentHRP-Conjugate reagent10.0ml10.0ml10.0ml10.010.010.010.0mlmlml5.0ml5.0ml5.0ml5.0ml5.0ml5.0ml5.0ml20X Wash solution25ml15ml20X Wash solution20X Wash solution20X Wash solution20X W20X W20X W20X W20X Wash solutionash solutionash solutionash solution25ml25ml25ml25252525mlmlml15ml15ml15ml15ml15ml15ml15mlChromogen Solution A6.0ml3.0mlChromogen Solution AChromogen Solution AChromogen Solution AChromogen Solution AChromogen Solution AChromogen Solution A6.0ml6.0ml6.0ml666.0.0.0.0mlmlml3.0ml3.0ml3.0ml333.0ml.0ml.0ml.0mlChromogen Solution B6.0ml3.0mlChromogen Solution BChromogen Solution BChromogen Solution BChromogen Solution BChromogen Solution BChromogen Solution B6.0ml6.0ml6.0ml666.0.0.0.0mlmlml3.0ml3.0ml3.0ml333.0ml.0ml.0ml.0mlStop Solution6.0ml3.0mlStop SolutionStop SolutionStop SolutionStop SolutionStop SolutionStop Solution6.0ml6.0ml6.0ml666.0.0.0.0mlmlml3.0ml3.0ml3.0ml333.0ml.0ml.0ml.0mlClosure plate membrane22Closure plate membraneClosure plate membraneClosure plate membraneClosure plate membraneClosure plate membraneClosure plate membraneClosure plate membrane222222222222User manual11User manualUser manualUser manualUser manualUser manualUser manual111111111111Sealed bags11Sealed bagsSealed bagsSealed bagsSealed bagsSealed bagsSealed bagsSealed bags111111111111 Note: Standard (S0 → S5) concentration was followed by:0,20,40,80,160,320 pg/ml.Note: Standard (S0 → S5) concentration was followed by:0,20,40,80,160,320 pg/ml.Note: Note: Note: Note: Standard (Standard (Standard (S0S0S0S0 →  →  → S5S5S5S5) concentration was followed ) concentration was followed ) concentration was followed by:by:by:by:0,20,40,80,160,320 p0,20,40,80,160,320 p0,20,40,80,160,320 p0,20,40,80,160,320 p0,20,40,80,160,320 pg/ml.g/ml.g/ml.g/ml.g/ml.
Reagent preparationReagent preparationReagent preparationReagent preparationReagent preparationReagent preparationReagent preparation
20×wash solution:Dilute with Distilled or deionized water1:20.20×wash solution:Dilute with Distilled or deionized water1:20.20×wash solution:Dilute with Distilled or deionized water1:20.20×wash solution:Dilute with Distilled or deionized water1:20.20202020×××wash solution:Dilute with wash solution:Dilute with wash solution:Dilute with wash solution:Dilute with Distilled or deionized waterDistilled or deionized waterDistilled or deionized waterDistilled or deionized water1:20.1:20.1:20.1:20.1:20.
Assay procedureAssay procedureAssay procedureAAAAAAssay proceduressay proceduressay proceduressay proceduressay proceduressay proceduressay procedure
1. Prepare allreagentsbeforestartingassayprocedure. ItisrecommendedthatallStandardsand Samplesbe addedin duplicateto the MicroelisaStripplate.1. Prepare allreagentsbeforestartingassayprocedure. ItisrecommendedthatallStandardsand Samplesbe addedin duplicateto the MicroelisaStripplate.1. Prepare allreagentsbeforestartingassayprocedure. ItisrecommendedthatallStandardsand Samplesbe addedin duplicateto the MicroelisaStripplate.1. Prepare allreagentsbeforestartingassayprocedure. ItisrecommendedthatallStandardsand Samplesbe addedin duplicateto the MicroelisaStripplate.1. 1. 1. 1. 1. PPPPPrrrrrrepepepepepaaaaaarrrrrre ae ae ae ae alllllllllll reagentreagentreagentreagentreagentreagentsssss bebebebebeffffffooooorrrrrreeeee ststststststaaaaartirtirtirtirtirtingngngngng aaaaassssssssssssaaaaaayyyyy ppppprrrrrroceduoceduoceduoceduocedurrrrrre.e.e.e.e. I I I I I Ittttt iiiiiisssss rrrrrrecoecoecoecoecommmmmmmmmmmmendedendedendedendedended tttttthhhhhaaaaaattttt aaaaalllllllllll SSSSSttttttandandandandandaaaaaarrrrrrdsdsdsdsds and Sand Sand Sand Sand Saaaaaammmmmmppppplllllleseseseses be addedbe addedbe addedbe addedbe added iiiiiin dupn dupn dupn dupn duplilililililicccccaaaaaatttttteeeee tttttto o o o o tttttthe he he he he MiMiMiMiMiMicccccrrrrrroooooelisaelisaelisaelisaelisa StrippStrippStrippStrippStrippStrippllllllaaaaaatttttte.e.e.e.e.
2. Add standard: Set Standard wells, testing sample wells. Add standard 50μl to standard well.2. Add standard: Set Standard wells, testing sample wells. Add standard 50μl to standard well.2. Add standard: Set Standard wells, testing sample wells. Add standard 50μl to standard well.2. 2. 2. 2. 2. Add Add Add Add standardstandardstandard: : : : Set Standard wellsSet Standard wellsSet Standard wells, , , , testing sample welltesting sample welltesting sample wells. s. s. s. Add standard 50μl to standard wellAdd standard 50μl to standard wellAdd standard 50μl to standard well....
3. Add Sample: Add testing sample10μl then add Sample Diluent 40μl to testing sample well; Blank welldoesn’t add anyting.3. Add Sample: Add testing sample10μl then add Sample Diluent 40μl to testing sample well; Blank welldoesn’t add anyting.3. Add Sample: Add testing sample10μl then add Sample Diluent 40μl to testing sample well; Blank welldoesn’t add anyting.3. 3. 3. 3. 3. 3. Add Add Add Add SampleSampleSample: Add testing s: Add testing s: Add testing s: Add testing sampleampleample11110μl 0μl 0μl then athen athen athen add dd dd Sample Diluent 40Sample Diluent 40Sample Diluent 40Sample Diluent 40μl to testing sample wellμl to testing sample wellμl to testing sample well; B; B; B; Blank welllank welllank welldoesndoesndoesndoesn’’’t add anyting.t add anyting.t add anyting.t add anyting.
4. Add100μlofHRP-conjugate reagentto each well,cover with an adhesive stripandincubatefor60 minutes at37°C.4. Add100μlofHRP-conjugate reagentto each well,cover with an adhesive stripandincubatefor60 minutes at37°C.4. Add100μlofHRP-conjugate reagentto each well,cover with an adhesive stripandincubatefor60 minutes at37°C.4. 4. 4. 4. 4. 4. 4. AAAAAAdddddddddd 1010101010100μ0μ0μ0μ0μlllll ofofofofof HRP-conjugate reagentHRP-conjugate reagentHRP-conjugate reagentto each wellto each wellto each wellto each well,,, cccover with aover with aover with aover with annnnn adhesive strip adhesive strip adhesive strip adhesive strip andandandandand iiiiiincubncubncubncubncubaaaaaatttttteeeee ffffffororororor 6666660 minutes0 minutes0 minutes0 minutes0 minutes at at at at at 33333777777°°°°°CCCCCC..... 
5. Aspirate each well and wash, repeating the process four times for a total of five washes.Wash by filling each well with Wash Solution (400μl) using a squirt bottle, manifolddispenseror autowasher. Complete removal of liquid at each step is essential to good performance. After the last wash, remove any remaining Wash Solution by aspirating ordecanting. Invert the plate and blot it against clean paper towels.5. Aspirate each well and wash, repeating the process four times for a total of five washes.Wash by filling each well with Wash Solution (400μl) using a squirt bottle, manifolddispenseror autowasher. Complete removal of liquid at each step is essential to good performance. After the last wash, remove any remaining Wash Solution by aspirating ordecanting. Invert the plate and blot it against clean paper towels.5. Aspirate each well and wash, repeating the process four times for a total of five washes.Wash by filling each well with Wash Solution (400μl) using a squirt bottle, manifolddispenseror autowasher. Complete removal of liquid at each step is essential to good performance. After the last wash, remove any remaining Wash Solution by aspirating ordecanting. Invert the plate and blot it against clean paper towels.555555. . . . . Aspirate each well and wash, repeating the process Aspirate each well and wash, repeating the process Aspirate each well and wash, repeating the process Aspirate each well and wash, repeating the process fourfourfourfour times for a total of f times for a total of f times for a total of f times for a total of five ive ive ive washes.washes.washes.washes. Wash by filling each well with Wash Wash by filling each well with Wash Wash by filling each well with Wash Wash by filling each well with Wash SolutionSolutionSolutionSolution (400 (400 (400 (400μμμμμlllll) using a squirt bottle, manifold) using a squirt bottle, manifold) using a squirt bottle, manifold) using a squirt bottle, manifold dispenserdispenserdispenserdispenser or autowasher. Complete removal of liquid at each step is essential to gooor autowasher. Complete removal of liquid at each step is essential to gooor autowasher. Complete removal of liquid at each step is essential to gooor autowasher. Complete removal of liquid at each step is essential to good d d d performance. After the last wash, remove any remaining Wash performance. After the last wash, remove any remaining Wash performance. After the last wash, remove any remaining Wash performance. After the last wash, remove any remaining Wash SolutionSolutionSolutionSolution by aspirating or by aspirating or by aspirating or by aspirating or decanting. Invert the plate and blot it against clean paper towels.decanting. Invert the plate and blot it against clean paper towels.decanting. Invert the plate and blot it against clean paper towels.decanting. Invert the plate and blot it against clean paper towels.
6. Add chromogen solution A 50μl and chromogen solution B 50μl to each well. Gently mix and incubate for 15 minutes at 37°C. Protect from light.6. Add chromogen solution A 50μl and chromogen solution B 50μl to each well. Gently mix and incubate for 15 minutes at 37°C. Protect from light.6. Add chromogen solution A 50μl and chromogen solution B 50μl to each well. Gently mix and incubate for 15 minutes at 37°C. Protect from light.666666. . . . . Add chromogen solution A 50μl and chromogen solution B 50μl to each well.Add chromogen solution A 50μl and chromogen solution B 50μl to each well.Add chromogen solution A 50μl and chromogen solution B 50μl to each well. Gently mix  Gently mix  Gently mix  Gently mix and and and and incubate for 15 minutes at incubate for 15 minutes at incubate for 15 minutes at incubate for 15 minutes at 3737373737°C°C°C°C°C. . . Protect from lightProtect from lightProtect from lightProtect from lightProtect from light.....
7. Add 50μl Stop Solution to each well. The color in the wells should change from blue toyellow. If the color in the wells is green or the color change does not 7. Add 50μl Stop Solution to each well. The color in the wells should change from blue toyellow. If the color in the wells is green or the color change does not 7. Add 50μl Stop Solution to each well. The color in the wells should change from blue toyellow. If the color in the wells is green or the color change does not 7. Add 50μl Stop Solution to each well. The color in the wells should change from blue toyellow. If the color in the wells is green or the color change does not 777777. . . . . Add 50Add 50Add 50Add 50μμμμμl l l l l Stop Solution to each well. The color in the wells should change from blue toStop Solution to each well. The color in the wells should change from blue toStop Solution to each well. The color in the wells should change from blue toStop Solution to each well. The color in the wells should change from blue to yellow. If the color in the wells is green or the color change does not yellow. If the color in the wells is green or the color change does not yellow. If the color in the wells is green or the color change does not yellow. If the color in the wells is green or the color change does not
appear uniform,gently tap the plate to ensure thorough mixing.appear uniform,gently tap the plate to ensure thorough mixing.appear uniform,gently tap the plate to ensure thorough mixing.appear uniform,gently tap the plate to ensure thorough mixing.appear uniform,appear uniform,appear uniform,appear uniform, gently tap the plate to ensure thorough mixinggently tap the plate to ensure thorough mixinggently tap the plate to ensure thorough mixinggently tap the plate to ensure thorough mixing....
8. ReadtheOpticalDensity(O.D.)at450nmusinga microtiterplatereaderwithin15minutes.8. ReadtheOpticalDensity(O.D.)at450nmusinga microtiterplatereaderwithin15minutes.8. ReadtheOpticalDensity(O.D.)at450nmusinga microtiterplatereaderwithin15minutes.888888. . . . . RRRRRRRReadeadeadeadeadeadead tttttttthehehehehehehe OOOOOOOOppppppptitititititititicalcalcalcalcalcalcal DDDDDDDDenenenenenenensitsitsitsitsitsitsitsityyyyyyy ((((((((OOOOOOOO.......DDDDDDDD.).).).).).).) atatatatatatat 450450450450450450450 nmnmnmnmnmnmnm uuuuuuusisisisisisisisingngngngngngng a a a a a a a mmmmmmmmiiiiiiiicccccccrrrrrrrroooooootittittittittittittittitererererererer pppppppllllllllaaaaaaaatttttttteeeeeee rrrrrrrreadereadereadereadereadereadereader witwitwitwitwitwitwitwithhhhhhhiiiiiiiinnnnnnn 1515151515151515 mmmmmmmmiiiiiiiinununununununutttttttteeeeeeessssssss.......
Calculation of resultsCalculation of resultsCalculation of resultsCCCCCCCCalculation of resultsalculation of resultsalculation of resultsalculation of resultsalculation of resultsalculation of resultsalculation of resultsalculation of resultsalculation of results

1. This standard curve is used to determine the amount in an unknown sample. The standard curve is generated by plotting the average O.D. (450 nm) obtained for each of the six standard concentrations on the vertical (Y) axis versus the corresponding concentration on the horizontal (X) axis.
2. First, calculate the mean O.D. value for each standard and sample. All O.D. values, are subtracted by the mean value of the zero standard before result interpretation. Construct the standard curve using graph paper or statistical software.
3. To determine the amount in each sample, first locate the O.D. value on the Y-axis and extend a horizontal line to the standard curve. At the point of intersection, draw a vertical line to the X-axis and read the corresponding concentration.
4. Any variation in operator, pipetting and washing technique, incubation time or temperature, and kit age can cause variation in result. Each user should obtain their own standard curve.
5. The sensitivity by this assay is1.0 pg/ml
6. Standard curve

This standard curve is used to determine the amount in an unknown sample. The standard curve is generated by plotting the average O.D. (450 nm) obtained for each of the six standard concentrations on the vertical (Y) axis versus the corresponding concentration on the horizontal (X) axis.

This standard curve is used to determine the amount in an unknown sample. The standard curve is generated by plotting the average O.D. (450 nm) obtained for each of the six standard concentrations on the vertical (Y) axis versus the corresponding concentration on the horizontal (X) axis. This standard curve is used to determine the amount in an unknown sample. The standard curve is generated by plotting the average O.D. (450 nm) obtained for each of the six standard concentrations on the vertical (Y) axis versus the corresponding concentration on the horizontal (X) axis. This standard curve is used to determine the amount in an unknown sample. The standard curve is generated by plotting the average O.D. (450 nm) obtained for each of the six standard concentrations on the vertical (Y) axis versus the corresponding concentration on the horizontal (X) axis. This standard curve is used to determine the amount in an unknown sample. The standard curve is generated by plotting the average O.D. (450 nm) obtained for each of the six standard concentrations on the vertical (Y) axis versus the corresponding concentration on the horizontal (X) axis. This standard curve is used to determine the amount in an unknown sample. The standard curve is generated by plotting the average O.D. (450 nm) obtained for each of the six standard concentrations on the vertical (Y) axis versus the corresponding concentration on the horizontal (X) axis. This standard curve is used to determine the amount in an unknown sample. The standard curve is generated by plotting the average O.D. (450 nm) obtained for each of the six standard concentrations on the vertical (Y) axis versus the corresponding concentration on the horizontal (X) axis. This standard curve is used to determine the amount in an unknown sample. The standard curve is generated by plotting the average O.D. (450 nm) obtained for each of the six standard concentrations on the vertical (Y) axis versus the corresponding concentration on the horizontal (X) axis. This standard curve is used to determine the amount in an unknown sample. The standard curve is generated by plotting the average O.D. (450 nm) obtained for each of the six standard concentrations on the vertical (Y) axis versus the corresponding concentration on the horizontal (X) axis.

First, calculate the mean O.D. value for each standard and sample. All O.D. values, are subtracted by the mean value of the zero standard before result interpretation. Construct the standard curve using graph paper or statistical software.

First, calculate the mean O.D. value for each standard and sample. All O.D. values, are subtracted by the mean value of the zero standard before result interpretation. Construct the standard curve using graph paper or statistical software. First, calculate the mean O.D. value for each standard and sample. All O.D. values, are subtracted by the mean value of the zero standard before result interpretation. Construct the standard curve using graph paper or statistical software. First, calculate the mean O.D. value for each standard and sample. All O.D. values, are subtracted by the mean value of the zero standard before result interpretation. Construct the standard curve using graph paper or statistical software. First, calculate the mean O.D. value for each standard and sample. All O.D. values, are subtracted by the mean value of the zero standard before result interpretation. Construct the standard curve using graph paper or statistical software. First, calculate the mean O.D. value for each standard and sample. All O.D. values, are subtracted by the mean value of the zero standard before result interpretation. Construct the standard curve using graph paper or statistical software. First, calculate the mean O.D. value for each standard and sample. All O.D. values, are subtracted by the mean value of the zero standard before result interpretation. Construct the standard curve using graph paper or statistical software. First, calculate the mean O.D. value for each standard and sample. All O.D. values, are subtracted by the mean value of the zero standard before result interpretation. Construct the standard curve using graph paper or statistical software. First, calculate the mean O.D. value for each standard and sample. All O.D. values, are subtracted by the mean value of the zero standard before result interpretation. Construct the standard curve using graph paper or statistical software.

To determine the amount in each sample, first locate the O.D. value on the Y-axis and extend a horizontal line to the standard curve. At the point of intersection, draw a vertical line to the X-axis and read the corresponding concentration.

To determine the amount in each sample, first locate the O.D. value on the Y-axis and extend a horizontal line to the standard curve. At the point of intersection, draw a vertical line to the X-axis and read the corresponding concentration. To determine the amount in each sample, first locate the O.D. value on the Y-axis and extend a horizontal line to the standard curve. At the point of intersection, draw a vertical line to the X-axis and read the corresponding concentration. To determine the amount in each sample, first locate the O.D. value on the Y-axis and extend a horizontal line to the standard curve. At the point of intersection, draw a vertical line to the X-axis and read the corresponding concentration. To determine the amount in each sample, first locate the O.D. value on the Y-axis and extend a horizontal line to the standard curve. At the point of intersection, draw a vertical line to the X-axis and read the corresponding concentration. To determine the amount in each sample, first locate the O.D. value on the Y-axis and extend a horizontal line to the standard curve. At the point of intersection, draw a vertical line to the X-axis and read the corresponding concentration. To determine the amount in each sample, first locate the O.D. value on the Y-axis and extend a horizontal line to the standard curve. At the point of intersection, draw a vertical line to the X-axis and read the corresponding concentration. To determine the amount in each sample, first locate the O.D. value on the Y-axis and extend a horizontal line to the standard curve. At the point of intersection, draw a vertical line to the X-axis and read the corresponding concentration. To determine the amount in each sample, first locate the O.D. value on the Y-axis and extend a horizontal line to the standard curve. At the point of intersection, draw a vertical line to the X-axis and read the corresponding concentration.

Any variation in operator, pipetting and washing technique, incubation time or temperature, and kit age can cause variation in result. Each user should obtain their own standard curve.

Any variation in operator, pipetting and washing technique, incubation time or temperature, and kit age can cause variation in result. Each user should obtain their own standard curve.Any variation in operator, pipetting and washing technique, incubation time or temperature, and kit age can cause variation in result. Each user should obtain their own standard curve.Any variation in operator, pipetting and washing technique, incubation time or temperature, and kit age can cause variation in result. Each user should obtain their own standard curve.Any variation in operator, pipetting and washing technique, incubation time or temperature, and kit age can cause variation in result. Each user should obtain their own standard curve.Any variation in operator, pipetting and washing technique, incubation time or temperature, and kit age can cause variation in result. Each user should obtain their own standard curve.Any variation in operator, pipetting and washing technique, incubation time or temperature, and kit age can cause variation in result. Each user should obtain their own standard curve.Any variation in operator, pipetting and washing technique, incubation time or temperature, and kit age can cause variation in result. Each user should obtain their own standard curve.Any variation in operator, pipetting and washing technique, incubation time or temperature, and kit age can cause variation in result. Each user should obtain their own standard curve.

The sensitivity by this assay is1.0 pg/ml

The sensitivity by this assay is1.0 pg/mlThe sensitivity by this assay is1.0 pg/mlThe sensitivity by this assay is1.0 pg/mlThe sensitivity by this assay is1.0 pg/mlThe sensitivity by this assay isThe sensitivity by this assay isThe sensitivity by this assay isThe sensitivity by this assay is 1.0 pg/ml1.0 pg/ml1.0 pg/ml1.0 pg/ml1.0 pg/ml

Standard curve

Standard curveStandard curveStandard curveStandard curveStandard curveStandard curveStandard curveStandard curve

Storage： 2-8℃.Storage： 2-8℃.SSStorage： torage： toragetorage： ： 2-82-82-8℃.℃.℃℃..
validity：six months.validity：six months.validityvalidityvalidity：six months.：six months.：： six months.six months.
FOR RESEARCH USE ONLY;
NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!
PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!FOR RESEARCH USE ONLY;
NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!
PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!FOR RESEARCH USE ONLY;
NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!
PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!FOR RESEARCH USE ONLY;
NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!FOR RESEARCH USE ONLY;
NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!FOR RESEARCH USE ONLY;
NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!FOR RESEARCH USE ONLY;
NOT FOR THERAPEUTIC OR DIAGNOSTIC APPLICATIONS!
PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!PLEASE READ THROUGH ENTIRE PROCEDURE BEFORE BEGINNING!