MHC I STREPTAMER® 抗原特异性T 细胞分选

抗原特异性 (CD8+ T) 细胞与荧光标记的MHC I STREPTAMERS®（荧光标记的Strep-Tactin®通低亲和力的MHC I-Streps形成复合体）共染，可经流式细胞仪检测并筛选，随后通过生物素去除标签，从而维持了细胞的真实性。这些功能性的，label-free的细
胞可放心用于后续下游的实验研究。

MHC I Streptamer®由两部分组成：
› MHC I-Strep，即连接了Twin-Strep-tag®和抗原肽的MHC I分子。
› 荧光 (PE/APC) 或磁性微珠标记的Strep-Tactin® multimer

核心优点：
› 多价结合，提供了精准的标记和高染色强度
› 多种选择，可从60余种MHC I Streptamer® 与相应多肽进行选择
› 模块化产品，适用于FACS分析与磁性细胞分选
› 试剂可逆，保证了选后细胞的完整功能



Specifications

MHC I STREPTAMER® 抗原特异性T 细胞分选

抗原特异性 (CD8+ T) 细胞与荧光标记的MHC I STREPTAMERS®（荧光标记的Strep-Tactin®通低亲和力的MHC I-Streps形成复合体）共染，可经流式细胞仪检测并筛选，随后通过生物素去除标签，从而维持了细胞的真实性。这些功能性的，label-free的细
胞可放心用于后续下游的实验研究。

MHC I Streptamer®由两部分组成：
› MHC I-Strep，即连接了Twin-Strep-tag®和抗原肽的MHC I分子。
› 荧光 (PE/APC) 或磁性微珠标记的Strep-Tactin® multimer

核心优点：
› 多价结合，提供了精准的标记和高染色强度
› 多种选择，可从60余种MHC I Streptamer® 与相应多肽进行选择
› 模块化产品，适用于FACS分析与磁性细胞分选
› 试剂可逆，保证了选后细胞的完整功能



Specifications

Concentration:	See data sheet for specific Lot. No.
Form:	Dissolved in DPBS
Peptide Sequence:	VAPWNSFAL
Possible Application:	Fluorescent cell staining & sorting, magnetic or affinity chromatographic cell isolation
Recommended Usage:	0.2 µg per 2 x 10^6 total cells for fluorescent staining or magnetic isolation
Sterility:	Sterile
MHC I Allele:	HLA-C*0102

Concentration:See data sheet for specific Lot. No.Form:Dissolved in DPBSPeptide Sequence:VAPWNSFALPossible Application:Fluorescent cell staining & sorting, magnetic or affinity chromatographic cell isolationRecommended Usage:0.2 µg per 2 x 10^6 total cells for fluorescent staining or magnetic isolationSterility:SterileMHC I Allele:HLA-C*0102Concentration:See data sheet for specific Lot. No.Concentration:Concentration:See data sheet for specific Lot. No.See data sheet for specific Lot. No.Form:Dissolved in DPBSForm:Form:Dissolved in DPBSDissolved in DPBSPeptide Sequence:VAPWNSFALPeptide Sequence:Peptide Sequence:VAPWNSFALVAPWNSFALPossible Application:Fluorescent cell staining & sorting, magnetic or affinity chromatographic cell isolationPossible Application:Possible Application:Fluorescent cell staining & sorting, magnetic or affinity chromatographic cell isolationFluorescent cell staining & sorting, magnetic or affinity chromatographic cell isolationRecommended Usage:0.2 µg per 2 x 10^6 total cells for fluorescent staining or magnetic isolationRecommended Usage:Recommended Usage:0.2 µg per 2 x 10^6 total cells for fluorescent staining or magnetic isolation0.2 µg per 2 x 10^6 total cells for fluorescent staining or magnetic isolationSterility:SterileSterility:Sterility:SterileSterileMHC I Allele:HLA-C*0102MHC I Allele:MHC I Allele:HLA-C*0102HLA-C*0102

Shipping information

Shipping information

Storage:	Long-term: -80 °C, short-term: 2-8 °C
Stability:	12 months after shipping
Shipping:	Dry ice

Storage:Long-term: -80 °C, short-term: 2-8 °CStability:12 months after shippingShipping:Dry iceStorage:Long-term: -80 °C, short-term: 2-8 °CStorage:Storage:Long-term: -80 °C, short-term: 2-8 °CLong-term: -80 °C, short-term: 2-8 °CStability:12 months after shippingStability:Stability:12 months after shipping12 months after shippingShipping:Dry iceShipping:Shipping:Dry iceDry ice
MHC I-Strep HLA-C*0102; TIMP1 (VAPWNSFAL) is a recombinantly expressed MHC class I molecule that presents a peptide from a tissue inhibitor of metalloproteinases 1 (TIMP1). Our MHC I Streptamer^®MHC I Streptamer^®® approach is based on our Strep-tag^® technologyStrep-tag^®® technology and facilitates reversible antigen-specific T cell labeling. A Twin-Strep-tag^®® fused to the MHC I-Strep permits multimerization of MHC I complexes on a Strep-Tactin^®® backbone, resulting in the formation of MHC I Streptamers.


For multimerizing MHC I-Streps, Strep-Tactin^®® TACS Agarose columns, Strep-Tactin^®® Magnetic Microbeads or fluorophore-conjugated Strep-Tactin^®® can be used. This allows affinity chromatographic or magnetic isolation or cell staining for flow cytometry analysis and sorting (FACS), depending on the experimental requirements. The addition of biotin reverses the multimerization of MHC class I molecules, leading to their dissociation from the target cells. The resulting label-free cells are highly viable and fully functional.