TotalSeq™-A0564 抗小鼠叶酸受体 β (FR-β) 抗体、TotalSeq™-A0564 anti-mouse Folate Receptor β (FR-β) Antibody详细如下：
Description：
Folate receptor beta (FR-β) is a member of a family of folate binding receptors that have diverse structural identities but mediate transport of folates into cells. FR-β is a GPI-anchored protein with high affinity for folic acid. It is postulated that this receptor function as folate scavengers when folate supply is low or rapid cell growth requires elevated uptake of folate for methylation reactions including DNA biosynthesis. Although this protein was originally thought to be specific to placenta, it can also exist in other tissues, and it may play a role in the transport of methotrexate in synovial macrophages in rheumatoid arthritis patients.
Other Names：FR-β
Isotype：Rat IgG2a, κ
Isotype Control：TotalSeq™-A0238 Rat IgG2a, κ Isotype Ctrl
Reactivity：Mouse
Antibody Type：Monoclonal
Host Species：Rat
Immunogen：Mouse Folate receptor β transfected cells
Formulation：Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 1 mM EDTA.
Preparation：The antibody was purified by chromatography and conjugated with TotalSeq™-A oligomer under optimal conditions.
Concentration：0.5 mg/ml
Storage & Handling：The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application：PG - Quality tested
Recommended Usage：
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis and the oligomer sequence is confirmed by sequencing. TotalSeq™-A antibodies are compatible with 10x Genomics Single Cell Gene Expression Solutions.
To maximize performance, it is strongly recommended that the reagent be titrated for each application, and that you centrifuge the antibody dilution before adding to the cells at 14,000xg at 2 - 8°C for 10 minutes. Carefully pipette out the liquid avoiding the bottom of the tube and add to the cell suspension. For Proteogenomics analysis, the suggested starting amount of this reagent for titration is ≤ 1.0 µg per million cells in 100 µL volume. Refer to the corresponding TotalSeq™ protocol for specific staining instructions.
Buyer is solely responsible for determining whether Buyer has all intellectual property rights that are necessary for Buyer's intended uses of the BioLegend TotalSeq™ products. For example, for any technology platform Buyer uses with TotalSeq™, it is Buyer's sole responsibility to determine whether it has all necessary third party intellectual property rights to use that platform and TotalSeq™ with that platform.
Application References：
Nagai T, et al. 2009. Cancer Immunol Immunother. 1577-86.
Structure：30 kD, seven disulfide bonds
Distribution：Placenta, monocytes, tissue resident macrophages
Function：Transport of folate into cells
Ligand/Receptor：Folic Acid
Cell Type：Macrophages, Monocytes
Antigen References：
1. Elwood PC, 1989, J Biol Chem, 264:14893.
2. Shen F, et al, 1994, Biochemistry, 8;33:1209.
3. Yi YS, 2016, Immune Netw,16:337.TotalSeq™-A0564 抗小鼠叶酸受体 β (FR-β) 抗体、TotalSeq™-A0564 anti-mouse Folate Receptor β (FR-β) Antibody详细如下：
Description：
Folate receptor beta (FR-β) is a member of a family of folate binding receptors that have diverse structural identities but mediate transport of folates into cells. FR-β is a GPI-anchored protein with high affinity for folic acid. It is postulated that this receptor function as folate scavengers when folate supply is low or rapid cell growth requires elevated uptake of folate for methylation reactions including DNA biosynthesis. Although this protein was originally thought to be specific to placenta, it can also exist in other tissues, and it may play a role in the transport of methotrexate in synovial macrophages in rheumatoid arthritis patients.
Other Names：FR-β
Isotype：Rat IgG2a, κ
Isotype Control：TotalSeq™-A0238 Rat IgG2a, κ Isotype Ctrl
Reactivity：Mouse
Antibody Type：Monoclonal
Host Species：Rat
Immunogen：Mouse Folate receptor β transfected cells
Formulation：Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 1 mM EDTA.
Preparation：The antibody was purified by chromatography and conjugated with TotalSeq™-A oligomer under optimal conditions.
Concentration：0.5 mg/ml
Storage & Handling：The antibody solution should be stored undiluted between 2°C and 8°C. Do not freeze.
Application：PG - Quality tested
Recommended Usage：
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis and the oligomer sequence is confirmed by sequencing. TotalSeq™-A antibodies are compatible with 10x Genomics Single Cell Gene Expression Solutions.
To maximize performance, it is strongly recommended that the reagent be titrated for each application, and that you centrifuge the antibody dilution before adding to the cells at 14,000xg at 2 - 8°C for 10 minutes. Carefully pipette out the liquid avoiding the bottom of the tube and add to the cell suspension. For Proteogenomics analysis, the suggested starting amount of this reagent for titration is ≤ 1.0 µg per million cells in 100 µL volume. Refer to the corresponding TotalSeq™ protocol for specific staining instructions.
Buyer is solely responsible for determining whether Buyer has all intellectual property rights that are necessary for Buyer's intended uses of the BioLegend TotalSeq™ products. For example, for any technology platform Buyer uses with TotalSeq™, it is Buyer's sole responsibility to determine whether it has all necessary third party intellectual property rights to use that platform and TotalSeq™ with that platform.
Application References：
Nagai T, et al. 2009. Cancer Immunol Immunother. 1577-86.
Structure：30 kD, seven disulfide bonds
Distribution：Placenta, monocytes, tissue resident macrophages
Function：Transport of folate into cells
Ligand/Receptor：Folic Acid
Cell Type：Macrophages, Monocytes
Antigen References：
1. Elwood PC, 1989, J Biol Chem, 264:14893.
2. Shen F, et al, 1994, Biochemistry, 8;33:1209.
3. Yi YS, 2016, Immune Netw,16:337.