重组人神经调节蛋白1-A/Human NRG1-A/Heregulin-α
Recombinant Human Neuregulin-1/Heregulin Alpha (EGF Domain)

SYNONYMS

SYNONYMSNeuregulin-1, Heregulin Alpha, NRG1-A, NRG1 A.

INTRODUCTION

INTRODUCTIONNeuregulin/Heregulin is a family of structurally related polypeptide growth factors which are stemmed from alternatively spliced genes (NRG1, NRG2, NRG3 and NRG4). Thus far, there are more than 14 soluble and transmembrane proteins derived from the NRG1 gene. Proteolytic processing of the extracellular domain of the transmembrane NRG1 isoforms release soluble growth factors. These isoforms contain the heregulins (HRGs), glial growth factors (GGFs) and sensory and motor neuron-derived factor (SMDF). All these factors have the Ig and EGF-like domain, and are able to bind to ErbB3 and ErbB4 receptor tyrosin kinases. This binding stimulates erb3 and erb4 heterodimerization with erb2, promoting intrinsic kinase activity, which results in tyrosine phosphorylation. NRG1 isoforms act to induce the growth and differentiation of epithelial, neuronal, glial, and other types of cells.

DESCRIPTION

DESCRIPTIONRecombinant Human Neuregulin-1/Heregulin Alpha (EGF Domain) produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 65 amino acids and having a total molecular mass of 7.4kDa.
NRG1-A is purified by proprietary chromatographic techniques.

SOURCE

SOURCEEscherichia Coli.

PHYSICAL APPEARANCE

PHYSICAL APPEARANCESterile Filtered White lyophilized (freeze-dried) powder.

FORMULATION

FORMULATIONLyophilized from a 0.2µm filtered solution in 20mM PB, pH 6.0 and 150mM NaCl.

SOLUBILITY

SOLUBILITYIt is recommended to reconstitute the lyophilized NRG1-A in sterile 18M-cm H₂2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

STABILITY

STABILITYLyophilized NRG1-A although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution NRG1-A should be stored at 4°C between 2-7 days and for future use below -18°C.
Please prevent freeze-thaw cycles.

PURITY

PURITYGreater than 97.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.

AMINO ACID SEQUENCE

AMINO ACID SEQUENCESHLVKCAEKE KTFCVNGGEC FMVKDLSNPS RYLCKCQPGF TGARCTENVP MKVQNQEKAE ELYQK.

BIOLOGICAL ACTIVITY

BIOLOGICAL ACTIVITYThe ED₅₀50 was determined by the dose-dependent stimulation of the proliferation of human MCF-7 cells is less than 40 ng/ml, corresponding to a specific activity of > 2.5×10⁴4 units/mg.

SAFETY DATA SHEET

SAFETY DATA SHEET

SDS

SDSSDS

USAGE

USAGEProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.