【恶性胚胎横纹肌瘤】RD细胞【恶性胚胎横纹肌瘤】RD细胞贴壁细胞: 对于贴壁细胞应先吸(倒)尽培养基，吸的越干净越好，以免中和后加入的消化液，使强度减弱（或PBS洗1－3次）。50ml培养瓶加入消化液约1-3ml，按此比例进行消化，(根据经验)，晃动使消化液铺均匀置37度培养箱约2-5分钟，镜下见细胞收缩变圆或少数脱落后，轻轻振动瓶底使细胞全部脱落，加入2-3ml完全培养基后，轻轻吹打，使细胞基本成单个悬浮，然后分置其它无菌培养瓶内，加入完全培养基后继续培养或实验。【恶性胚胎横纹肌瘤】RD细胞培【恶性胚胎横纹肌瘤】RD细胞培悬浮细胞: 一般传代可直接将细胞原液分置其它培养瓶内，加入完全培养基继续培养，如要高浓度可先离心1000rpm，5min后加入完全培养基，轻轻吹匀后，分置其它培养瓶内加入完全培养基继续培养。需要指出的是，在不同的科研小组中这些术语的定义会有所不同。许多研究员不用细胞系这个词表示细胞群除非细胞经过了遗传改造。
【恶性胚胎横纹肌瘤】RD细胞培养基的配制【恶性胚胎横纹肌瘤】RD细胞培养基的配制

培养基	品牌	货号
D-MEM/F-12	GIBCO	12400024
Dulbecco's Modified Eagle Medium (D-MEM), powder (high glucose)	GIBCO	12800017
F-12 Nutrient Mixture (Ham) powder	GIBCO	21700075
Iscove's Modified Dulbecco's Medium (IMDM) powder	GIBCO	12200036
Leibovitz's L-15 Medium powder	GIBCO	41300039
McCOY's 5A	SIGMA	M4892
Minimum Essential Medium (MEM) Alpha Medium powder (MEMα) with ribonucleosides and deoxyribonucleosides	GIBCO	11900024
Minimum Essential Medium (MEM) Alpha Medium powder (MEMα) without ribonucleosides and deoxyribonucleosides	GIBCO	12000022
Minimum Essential Medium (MEM) powder	GIBCO	41500034
NUTRIENT MIXTURE F12 HAM KAIGHN'S MODIFICATION (F12K)	SIGMA	N3520
RPMI Medium 1640	GIBCO	31800022
EGF	SIGMA	E9644
Sodium pyruvate	SIGMA	P2256-25G
MEDIUM 199, WITH EARLE'S SALTS AND L-GLUTAMINE, WITHOUT SODIUM BICARBONATE. CELL CULTURE TESTED	SIGMA	M5017

培养基品牌货号D-MEM/F-12GIBCO12400024Dulbecco's Modified Eagle Medium (D-MEM), powder (high glucose)GIBCO12800017F-12 Nutrient Mixture (Ham) powderGIBCO21700075Iscove's Modified Dulbecco's Medium (IMDM) powderGIBCO12200036Leibovitz's L-15 Medium powderGIBCO41300039McCOY's 5A

SIGMA

M4892Minimum Essential Medium (MEM) Alpha Medium powder (MEMα)
with ribonucleosides and deoxyribonucleosidesGIBCO11900024Minimum Essential Medium (MEM) Alpha Medium powder (MEMα)
without ribonucleosides and deoxyribonucleosidesGIBCO12000022Minimum Essential Medium (MEM) powderGIBCO41500034NUTRIENT MIXTURE F12 HAM KAIGHN'S
MODIFICATION (F12K)SIGMAN3520RPMI Medium 1640GIBCO31800022EGFSIGMAE9644Sodium pyruvateSIGMAP2256-25GMEDIUM 199, WITH EARLE'S SALTS AND L-GLUTAMINE, WITHOUT SODIUM BICARBONATE. CELL CULTURE TESTEDSIGMAM5017培养基品牌货号培养基培养基品牌品牌货号货号D-MEM/F-12GIBCO12400024D-MEM/F-12D-MEM/F-12GIBCOGIBCO1240002412400024Dulbecco's Modified Eagle Medium (D-MEM), powder (high glucose)GIBCO12800017Dulbecco's Modified Eagle Medium (D-MEM), powder (high glucose)Dulbecco's Modified Eagle Medium (D-MEM), powder (high glucose)GIBCOGIBCO1280001712800017F-12 Nutrient Mixture (Ham) powderGIBCO21700075F-12 Nutrient Mixture (Ham) powderF-12 Nutrient Mixture (Ham) powderGIBCOGIBCO2170007521700075Iscove's Modified Dulbecco's Medium (IMDM) powderGIBCO12200036Iscove's Modified Dulbecco's Medium (IMDM) powderIscove's Modified Dulbecco's Medium (IMDM) powderGIBCOGIBCO1220003612200036Leibovitz's L-15 Medium powderGIBCO41300039Leibovitz's L-15 Medium powderLeibovitz's L-15 Medium powderGIBCOGIBCO4130003941300039McCOY's 5A

SIGMA

M4892McCOY's 5AMcCOY's 5A

SIGMA

SIGMA

SIGMASIGMASIGMASIGMAM4892M4892Minimum Essential Medium (MEM) Alpha Medium powder (MEMα)
with ribonucleosides and deoxyribonucleosidesGIBCO11900024Minimum Essential Medium (MEM) Alpha Medium powder (MEMα)
with ribonucleosides and deoxyribonucleosidesMinimum Essential Medium (MEM) Alpha Medium powder (MEMα)
with ribonucleosides and deoxyribonucleosidesGIBCOGIBCO1190002411900024Minimum Essential Medium (MEM) Alpha Medium powder (MEMα)
without ribonucleosides and deoxyribonucleosidesGIBCO12000022Minimum Essential Medium (MEM) Alpha Medium powder (MEMα)
without ribonucleosides and deoxyribonucleosidesMinimum Essential Medium (MEM) Alpha Medium powder (MEMα)
without ribonucleosides and deoxyribonucleosidesGIBCOGIBCO1200002212000022Minimum Essential Medium (MEM) powderGIBCO41500034Minimum Essential Medium (MEM) powderMinimum Essential Medium (MEM) powderGIBCOGIBCO4150003441500034NUTRIENT MIXTURE F12 HAM KAIGHN'S
MODIFICATION (F12K)SIGMAN3520NUTRIENT MIXTURE F12 HAM KAIGHN'S
MODIFICATION (F12K)NUTRIENT MIXTURE F12 HAM KAIGHN'S
MODIFICATION (F12K)SIGMASIGMAN3520N3520RPMI Medium 1640GIBCO31800022RPMI Medium 1640RPMI Medium 1640GIBCOGIBCO3180002231800022EGFSIGMAE9644EGFEGFSIGMASIGMAE9644E9644Sodium pyruvateSIGMAP2256-25GSodium pyruvateSodium pyruvateSIGMASIGMAP2256-25GP2256-25GMEDIUM 199, WITH EARLE'S SALTS AND L-GLUTAMINE, WITHOUT SODIUM BICARBONATE. CELL CULTURE TESTEDSIGMAM5017MEDIUM 199, WITH EARLE'S SALTS AND L-GLUTAMINE, WITHOUT SODIUM BICARBONATE. CELL CULTURE TESTEDMEDIUM 199, WITH EARLE'S SALTS AND L-GLUTAMINE, WITHOUT SODIUM BICARBONATE. CELL CULTURE TESTEDSIGMASIGMAM5017M5017 2、 按要求添加碳酸氢钠、谷氨酸钠、HEPES等，充分搅拌使之溶解。
3、 调pH至7.2左右。
4、 加水至最终体积。
5、 在超净台中对溶液进行滤过除菌，分装入250 mL或500 mL玻璃瓶中，用翻帽塞塞紧瓶口。
6、 瓶口封好，4℃冰箱贮存。
【恶性胚胎横纹肌瘤】RD细胞【恶性胚胎横纹肌瘤】RD细胞更多产品信息：更多产品信息：xy-E11589 人螺旋肽(THP)ELISA试剂盒xy-E11590 人溶酶体相关膜蛋白2(HLAMP-2)ELISA试剂盒xy-E11591 人软骨糖蛋白39(HC gp-39)ELISA试剂盒xy-E11592 人不透光相关蛋白(OPAs)ELISA试剂盒xy-E11593 人鞭毛蛋白(flagellin)ELISA试剂盒xy-E11594 人多聚血清蛋白(PHSA)ELISA试剂盒xy-E11595 人胞浆免疫球蛋白(CIg)ELISA试剂盒xy-E11596 人纤维介素蛋白(fgl2)ELISA试剂盒xy-E11597 人泛素蛋白(Ub)ELISA试剂盒xy-E11598 人类粘蛋白(ORM)ELISA试剂盒xy-E11599 人弹性蛋白(ELN)ELISA试剂盒xy-E11600 人套膜蛋白(iNV)ELISA试剂盒xy-E11601 人肌腱蛋白R(TN-R)ELISA试剂盒xy-E11602 人生长相关蛋白43(GAP-43)ELISA试剂盒xy-E11603 人cAMP反应元件结合蛋白(CREB)ELISA试剂盒xy-E11604 人聚集蛋白(Agrin)ELISA试剂盒xy-E11605 人Agouti相关蛋白(AGRP)ELISA试剂盒xy-E11606 人膜辅蛋白(MCP/CD46)ELISA试剂盒xy-E11607 人延伸蛋白A(EloA)ELISA试剂盒xy-E11608 人酰化刺激蛋白(ASP)ELISA试剂盒xy-E11609 人杀菌性/通透性增加蛋白(BPI)ELISA试剂盒xy-E11610 人细胞外基质磷酸糖蛋白(MEPE)ELISA试剂盒xy-E11611 人复制蛋白A(RPA-70)ELISA试剂盒xy-E11612 人软骨寡聚基质蛋白(COMP)ELISA试剂盒xy-E11613 人抗原提呈相关转运蛋白/T细胞活化蛋白(TAP)ELISA试剂盒xy-E11614 人免疫抑制酸性蛋白(IAP)ELISA试剂盒xy-E11615 人麦角蛋白IgA(Gliadin-IgA)ELISA试剂盒xy-E11616 人发动蛋白2(DNM2)ELISA试剂盒xy-E11617 人着丝粒蛋白B(CENP-B)ELISA试剂盒xy-E11618 人巨噬细胞刺激蛋白(MSP)ELISA试剂盒xy-E11619 人雷帕霉素靶蛋白(mTOR)ELISA试剂盒xy-E11620 人核基质蛋白22(NMP-22)ELISA试剂盒xy-E11621 人软骨寡聚蛋白(COMP)ELISA试剂盒xy-E11622 人多药耐药相关蛋白(MRP)ELISA试剂盒xy-E11623 人高速泳动蛋白17(HMG-17)ELISA试剂盒xy-E11624 人空泡毒素相关蛋白A(VacA)ELISA试剂盒xy-E11625 人玻连蛋白/体外粘连蛋白(VN/CD51+CD61)ELISA试剂盒xy-E11626 人波形蛋白(VIM)ELISA试剂盒xy-E11627 人表面活性蛋白D(SP-D)ELISA试剂盒xy-E11628 人阿拉伯半乳糖蛋白(AGPs)ELISA试剂盒xy-E11629 人β乳球蛋白(β-Lg)ELISA试剂盒xy-E11630 人β连环蛋白/联蛋白(β-Cat)ELISA试剂盒