重组人白介素13 (Human IL-13)
Recombinant Human Interleukin-13

SYNONYMS

SYNONYMSNC30, ALRH, BHR1, P600, IL-13, MGC116786, MGC116788, MGC116789.

INTRODUCTION

INTRODUCTIONIL13 is an immunoregulatory cytokine produced primarily by activated Th2 cells. IL-13 is involved in several stages of B-cell maturation and differentiation. It up-regulates CD23 and MHC class II expression, and promotes IgE isotype switching of B cells. This cytokine down-regulates macrophage activity, thereby inhibits the production of pro-inflammatory cytokines and chemokines. This cytokine is found to be critical to the pathogenesis of allergen-induced asthma but operates through mechanisms independent of IgE and eosinophils. This gene, IL3, IL5, IL4, and CSF2 form a cytokine gene cluster on chromosome 5q, with this gene particularly close to IL4.

DESCRIPTION

DESCRIPTIONInterleukin-13 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 112 amino acids and having a molecular mass of 12 kDa.
The IL-13 is purified by proprietary chromatographic techniques.

SOURCE

SOURCEEscherichia Coli.

PHYSICAL APPEARANCE

PHYSICAL APPEARANCESterile Filtered White lyophilized (freeze-dried) powder.

FORMULATION

FORMULATIONThe protein (1mg/ml) was lyophilized with 1xPBS pH-7.2 & 5% trehalose.

SOLUBILITY

SOLUBILITYIt is recommended to reconstitute the lyophilized Interleukin 13 in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

STABILITY

STABILITYLyophilized Interleukin-13 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IL13 should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.

PURITY

PURITYGreater than 95% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.

AMINO ACID SEQUENCE

AMINO ACID SEQUENCEGPVPPSTALRELIEELVNITQNQKAPLCNGSMVWSINLTAGMYCAALESLINVS
GCSAIEKTQRMLSGFCPHKVSAGQFSSLHVRDTKIEVAQFVKDLLLHLKKLFRE
GRFN.

BIOLOGICAL ACTIVITY

BIOLOGICAL ACTIVITYThe ED50 was determined by the dose dependent prolifiration of TF-1 cells and was found to be < 1ng/ml, corresponding to a specific activity of >1 x 10⁶6units/mg.

PROTEIN CONTENT

PROTEIN CONTENTProtein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280 nm using the absorbency value of 0.57 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
2. Analysis by RP-HPLC, using a calibrated solution of IL-13 as a Reference Standard.

SAFETY DATA SHEET

SAFETY DATA SHEET

SDS

SDSSDS

USAGE

USAGEProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.