重组小鼠脑多巴胺神经营养因子/Recombinant Murine CDNF
Recombinant Murine Cerebral Dopamine Neurotrophic Factor
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技术参数技术参数技术参数
FAQsFAQsFAQs
- Accession
- Q8CC36
AccessionAccession
Q8CC36Q8CC36Q8CC36
- GeneID
- 227526
GeneIDGeneID
227526227526227526
- Source
- Escherichia coli.
SourceSource
Escherichia coli.Escherichia coli.
- Molecular Weight
- Approximately 18.5 kDa, a single non-glycosylated polypeptide chain containing 163 amino acids.
Molecular WeightMolecular Weight
Approximately 18.5 kDa, a single non-glycosylated polypeptide chain containing 163 amino acids.Approximately 18.5 kDa, a single non-glycosylated polypeptide chain containing 163 amino acids.
- AA Sequence
- QGLEAGVGPR ADCEVCKEFL DRFYNSLLSR GIDFSADTIE KELLNFCSDA KGKENRLCYY LGATTDAATK ILGEVTRPMS VHIPAVKICE KLKKMDSQIC ELKYGKKLDL ASVDLWKMRV AELKQILQRW GEECRACAEK SDYVNLIREL APKYVEIYPQ TEL
AA SequenceAA Sequence
QGLEAGVGPR ADCEVCKEFL DRFYNSLLSR GIDFSADTIE KELLNFCSDA KGKENRLCYY LGATTDAATK ILGEVTRPMS VHIPAVKICE KLKKMDSQIC ELKYGKKLDL ASVDLWKMRV AELKQILQRW GEECRACAEK SDYVNLIREL APKYVEIYPQ TELQGLEAGVGPR ADCEVCKEFL DRFYNSLLSR GIDFSADTIE KELLNFCSDA KGKENRLCYY LGATTDAATK ILGEVTRPMS VHIPAVKICE KLKKMDSQIC ELKYGKKLDL ASVDLWKMRV AELKQILQRW GEECRACAEK SDYVNLIREL APKYVEIYPQ TEL
- Purity
- > 97 % by SDS-PAGE and HPLC analyses.
PurityPurity
> 97 % by SDS-PAGE and HPLC analyses.> 97 % by SDS-PAGE and HPLC analyses.
- Biological Activity
- Fully biologically active when compared to standard. It is able to enhance neurite outgrowth of E16-E18 rat embryonic cortical neurons when immobilized at 5 - 30 µg/mL on a nitrocellulose-coated microplate.
Biological ActivityBiological Activity
Fully biologically active when compared to standard. It is able to enhance neurite outgrowth of E16-E18 rat embryonic cortical neurons when immobilized at 5 - 30 µg/mL on a nitrocellulose-coated microplate.Fully biologically active when compared to standard. It is able to enhance neurite outgrowth of E16-E18 rat embryonic cortical neurons when immobilized at 5 - 30 µg/mL on a nitrocellulose-coated microplate.
- Physical Appearance
- Sterile Filtered White lyophilized (freeze-dried) powder.
Physical AppearancePhysical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.Sterile Filtered White lyophilized (freeze-dried) powder.
- Formulation
- Lyophilized from a 0.2 µm filtered concentrated solution in PBS, pH 7.4.
FormulationFormulation
Lyophilized from a 0.2 µm filtered concentrated solution in PBS, pH 7.4.Lyophilized from a 0.2 µm filtered concentrated solution in PBS, pH 7.4.
- Endotoxin
- Less than 0.1 EU/µg of rMuCDNF as determined by LAL method.
EndotoxinEndotoxin
Less than 0.1 EU/µg of rMuCDNF as determined by LAL method.Less than 0.1 EU/µg of rMuCDNF as determined by LAL method.
- Reconstitution
- We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
ReconstitutionReconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions.
- Stability & Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Stability & StorageStability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 3 months, -20 to -70 °C under sterile conditions after reconstitution.Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 3 months, -20 to -70 °C under sterile conditions after reconstitution.
- Usage
- NOT FOR HUMAN USE.
UsageUsage
NOT FOR HUMAN USE.NOT FOR HUMAN USE.
- SDS-PAGE
SDS-PAGESDS-PAGE
