重组人白介素2/Human IL 2, Yeast
Recombinant Human Interleukin-2, Yeast

SYNONYMS

SYNONYMS

Interleukin-2, T-cell growth factor (TCGF), Interleukin-2, Lymphokine, IL-2.

Interleukin-2, T-cell growth factor (TCGF), Interleukin-2, Lymphokine, IL-2.

INTRODUCTION

INTRODUCTIONIL2 is a secreted cytokine that is important for the proliferation of T and B lymphocytes. The receptor of this cytokine is a heterotrimeric protein complex whose gamma chain is also shared by interleukin 4 (IL4) and interleukin 7 (IL7). The expression of this gene in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. The targeted disruption of a similar gene in mice leads to ulcerative colitis-like disease, which suggests an essential role of this gene in the immune response to antigenic stimuli.

DESCRIPTION

DESCRIPTIONInterleukin-2 Human Recombinant produced in yeast is a single, glycosylated polypeptide chain containing 134 amino acids and having a molecular mass of 14 kDa. The IL-2 is purified by proprietary chromatographic techniques.

SOURCE

SOURCEPichia pastoris.

PHYSICAL APPEARANCE

PHYSICAL APPEARANCESterile Filtered White lyophilized (freeze-dried) powder.

FORMULATION

FORMULATIONThe protein was lyophilized from a 0.2µm filtered solution in 20mM sodium phosphate buffer pH 7.0 in absence of any carrier protein.

SOLUBILITY

SOLUBILITYIt is recommended to reconstitute the lyophilized Interleukin-2 in sterile 18M-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

STABILITY

STABILITYLyophilized Interleukin-2 although stable at room temperature for 3 weeks, should be stored desiccated below -18C. Upon reconstitution Interleukin-2 should be stored at 4C between 2-7 days and for future use below -18C.
Please prevent freeze-thaw cycles.

PURITY

PURITYGreater than 98% as determined by SDS-PAGE.

AMINO ACID SEQUENCE

AMINO ACID SEQUENCEA P T S S S T K K T Q L Q L E H L L L D L Q M I L N G I N N Y K N P K L T R M L T F K F Y M P K K A T E L K H L Q C L E E E L K P L E E V L N L A Q S K N F H L R P R D L I S N I N V I V L E L K G S E T T F M C E Y A D E T A T I V E F L N R W I T F C Q S I I S T L T.

BIOLOGICAL ACTIVITY

BIOLOGICAL ACTIVITYThe biological activity is determined by the dose dependent proliferation of mouse CTLL–2 cells. Optimal concentration for individual application should be determined by a dose response assay. ED50 range = 0.08–0.5ng/ml

SAFETY DATA SHEET

SAFETY DATA SHEET

SDS

SDSSDS

USAGE

USAGEProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.