The Ambion™ MEGAscript™ RNAi Kit is for the synthesis of large mass amounts of dsRNA for RNAi experiments in nonmammalian systems. The MEGAscript RNAi Kit uses Ambion's patented high-yield transcription technology and includes reagents for transcription, nuclease digestion, and clean up. The kit includes sufficient reagents for 20 reactions.

Synthesize Large Amounts of dsRNA:Synthesize Large Amounts of dsRNA:
The ability to generate large amounts of clean, double-stranded RNA is essential for the success of RNAi experiments. The MEGAscript RNAi Kit allows RNAi researchers to synthesize 50 to 100 µg or more of double-stranded RNA in a single transcription reaction. The yield is typically 10–50 times the amount of RNA produced by conventional transcription reactions. DNA of either PCR or plasmid origin can be used as a template for the transcription reaction. The sense and antisense strands can be synthesized from a PCR-generated DNA template containing T7 RNA Polymerase promoters on both ends of the template, eliminating the need for a separate annealing step.
For Research Use Only. Not for use in diagnostic procedures.

规格

规格
控制类型
转录对照
样品类型
双链 RNA（长）
反应次数
20
产品线
Ambion™、MEGAscript™
运输条件
干冰

内容与储存

内容与储存
T7 酶混合物、10X T7 反应缓冲液、ATP 溶液、CTP 溶液、GTP 溶液、UTP 溶液、10X 结合缓冲液、洗脱液、RNase、DNase I、10X 酶切缓冲液、2X 洗涤液、对照模板均储存在 –20°C 下，无核酸酶水可储存在任何温度。滤芯和收集管在室温下储存。
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