重组小鼠白介素7/Mouse IL-7
Recombinant Mouse Interleukin-7

SYNONYMS

SYNONYMSLymphopoietin 1 (LP-1), pre-B cell factor, IL-7.

INTRODUCTION

INTRODUCTIONInterleukin-7 is a potent lymphoid cell growth factor produced primarily by stromal cells. IL-7 has been shown to support the proliferation and differentiation of pre B- and early T cells as well as displaying a biological effect on cells of NK and myeloid lineages.

DESCRIPTION

DESCRIPTIONInterleukin-7 Mouse Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 129 amino acids and having a molecular mass of 14.9kDa.

SOURCE

SOURCEEscherichia Coli.

PHYSICAL APPEARANCE

PHYSICAL APPEARANCESterile Filtered White lyophilized (freeze-dried) powder.

FORMULATION

FORMULATIONFiltered (0.2µm) and lyophilized from a concentrated (1mg/ml) solution in 1×PBS, pH7.4 and 2% trehalose.

SOLUBILITY

SOLUBILITYIt is recommended to reconstitute the lyophilized Interleukin 7 in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

STABILITY

STABILITYLyophilized Interleukin-7 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IL7 should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.

PURITY

PURITYGreater than 96.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.

AMINO ACID SEQUENCE

AMINO ACID SEQUENCEECHIKDKEGK AYESVLMISI DELDKMTGTD SNCPNNEPNF FRKHVCDDTK EAAFLNRAAR KLKQFLKMNI SEEFNVHLLT VSQGTQTLVN CTSKEEKNVK EQKKNDACFL KRLLREIKTC WNKILKGSI.

BIOLOGICAL ACTIVITY

BIOLOGICAL ACTIVITYThe ED₅₀50was determined by the dose-dependent stimulation of the proliferation of murine 2E8 cells is less than 0.2 ng/ml, corresponding to a specific activity of >5.0×10⁶6 IU/mg.

REFERENCES

REFERENCESTitle: Disruption of Bis Leads to the Deterioration of the Vascular Niche for Hematopoietic Stem Cells
Publication: Stem Cells 28.2 (2010): 268-278.
Link: http://onlinelibrary.wiley.com/doi/10.1002/stem.285/fullhttp://onlinelibrary.wiley.com/doi/10.1002/stem.285/full

SAFETY DATA SHEET

SAFETY DATA SHEET

SDS

SDSSDS

USAGE

USAGEProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.