产品名称

Porcine HIF-1α(Hypoxia Inducible Factor 1 Alpha) ELISA Kit

别称

HIF-1α ELISA Kit, HIF1A ELISA Kit, ARNT-interacting protein ELISA Kit, Basic-helix-loop-helix-PAS protein MOP1 ELISA Kit, BHLHE78 ELISA Kit, Class E basic helix-loop-helix protein 78 ELISA Kit, HIF-1-alpha ELISA Kit, HIF1-alpha ELISA Kit, hypoxia-inducible factor 1-alpha ELISA Kit, Member of PAS ELISA Kit

检测方法

Sandwich ELISA, Double Antibody

应用

HIF-1α ELISA Kit allows for the in vitro quantitative determination of HIF1α concentrations in serum, plasma, tissue homogenates and other biological fluids.

规格

48T/96T

检测范围

0.156-10ng/ml

灵敏度

< 0.094ng/ml

种属

Porcine

UniProt ID

A0T1H6

标准曲线

存储

2-8 ℃ for 6 months

回收率

Matrices listed below were spiked with certain level of HIF1α and the recovery rates were calculated by comparing the measured value to the expected amount of HIF1α in samples.

Matrix	Recovery range(%)	Average(%)
serum(n=5)	91-105	95
EDTA plasma(n=5)	86-105	96
heparin plasma(n=5)	87-97	92

线性度

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of HIF1α and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1:2	1:4	1:8
serum(n=5)	85-103%	96-103%	85-101%
EDTA plasma(n=5)	90-99%	82-101%	87-99%
heparin plasma(n=5)	80-96%	82-99%	81-97%

变异系数（%）

批内差：CV<8%
批间差：CV<10%

注意事项

仅供科研使用

产品名称

产品名称

Porcine HIF-1α(Hypoxia Inducible Factor 1 Alpha) ELISA Kit

Porcine HIF-1α(Hypoxia Inducible Factor 1 Alpha) ELISA Kit

别称

别称

HIF-1α ELISA Kit, HIF1A ELISA Kit, ARNT-interacting protein ELISA Kit, Basic-helix-loop-helix-PAS protein MOP1 ELISA Kit, BHLHE78 ELISA Kit, Class E basic helix-loop-helix protein 78 ELISA Kit, HIF-1-alpha ELISA Kit, HIF1-alpha ELISA Kit, hypoxia-inducible factor 1-alpha ELISA Kit, Member of PAS ELISA Kit

HIF-1α ELISA KitHIF-1α ELISA Kit, HIF1A ELISA KitHIF1A ELISA Kit, ARNT-interacting protein ELISA KitARNT-interacting protein ELISA Kit, Basic-helix-loop-helix-PAS protein MOP1 ELISA KitBasic-helix-loop-helix-PAS protein MOP1 ELISA Kit, BHLHE78 ELISA KitBHLHE78 ELISA Kit, Class E basic helix-loop-helix protein 78 ELISA KitClass E basic helix-loop-helix protein 78 ELISA Kit, HIF-1-alpha ELISA KitHIF-1-alpha ELISA Kit, HIF1-alpha ELISA KitHIF1-alpha ELISA Kit, hypoxia-inducible factor 1-alpha ELISA Kithypoxia-inducible factor 1-alpha ELISA Kit, Member of PAS ELISA KitMember of PAS ELISA Kit

检测方法

检测方法

Sandwich ELISA, Double Antibody

Sandwich ELISA, Double Antibody

应用

应用

HIF-1α ELISA Kit allows for the in vitro quantitative determination of HIF1α concentrations in serum, plasma, tissue homogenates and other biological fluids.

HIF-1α ELISA KitHIF-1α ELISA Kit allows for the in vitro quantitative determination of HIF1α concentrations in serum, plasma, tissue homogenates and other biological fluids.

规格

规格

48T/96T

48T/96T

检测范围

检测范围

0.156-10ng/ml

0.156-10ng/ml

灵敏度

灵敏度

< 0.094ng/ml

< 0.094ng/ml

种属

种属

Porcine

Porcine

UniProt ID

UniProt ID

A0T1H6

A0T1H6A0T1H6

标准曲线

标准曲线

存储

存储

2-8 ℃ for 6 months

2-8 ℃ for 6 months

回收率

回收率

Matrices listed below were spiked with certain level of HIF1α and the recovery rates were calculated by comparing the measured value to the expected amount of HIF1α in samples.

Matrix	Recovery range(%)	Average(%)
serum(n=5)	91-105	95
EDTA plasma(n=5)	86-105	96
heparin plasma(n=5)	87-97	92

Matrices listed below were spiked with certain level of HIF1α and the recovery rates were calculated by comparing the measured value to the expected amount of HIF1α in samples.

Matrices listed below were spiked with certain level of HIF1α and the recovery rates were calculated by comparing the measured value to the expected amount of HIF1α in samples.

Matrix	Recovery range(%)	Average(%)
serum(n=5)	91-105	95
EDTA plasma(n=5)	86-105	96
heparin plasma(n=5)	87-97	92

MatrixRecovery range(%)Average(%)MatrixRecovery range(%)Average(%)MatrixMatrix Recovery range(%)Recovery range(%) Average(%)Average(%) serum(n=5)91-10595EDTA plasma(n=5)86-10596heparin plasma(n=5)87-9792serum(n=5)91-10595serum(n=5)serum(n=5) 91-10591-105 9595 EDTA plasma(n=5)86-10596EDTA plasma(n=5)EDTA plasma(n=5) 86-10586-105 9696 heparin plasma(n=5)87-9792heparin plasma(n=5)heparin plasma(n=5) 87-9787-97 9292

线性度

线性度

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of HIF1α and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1:2	1:4	1:8
serum(n=5)	85-103%	96-103%	85-101%
EDTA plasma(n=5)	90-99%	82-101%	87-99%
heparin plasma(n=5)	80-96%	82-99%	81-97%

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of HIF1α and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

The linearity of the kit was assayed by testing samples spiked with appropriate concentration of HIF1α and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.

Sample	1:2	1:4	1:8
serum(n=5)	85-103%	96-103%	85-101%
EDTA plasma(n=5)	90-99%	82-101%	87-99%
heparin plasma(n=5)	80-96%	82-99%	81-97%

Sample1:21:41:8Sample1:21:41:8SampleSample 1:21:2 1:41:4 1:81:8 serum(n=5)85-103%96-103%85-101%EDTA plasma(n=5)90-99%82-101%87-99%heparin plasma(n=5)80-96%82-99%81-97%serum(n=5)85-103%96-103%85-101%serum(n=5)serum(n=5) 85-103%85-103% 96-103%96-103% 85-101%85-101% EDTA plasma(n=5)90-99%82-101%87-99%EDTA plasma(n=5)EDTA plasma(n=5) 90-99%90-99% 82-101%82-101% 87-99%87-99% heparin plasma(n=5)80-96%82-99%81-97%heparin plasma(n=5)heparin plasma(n=5) 80-96%80-96% 82-99%82-99% 81-97%81-97%

变异系数（%）

变异系数（%）

批内差：CV<8%
批间差：CV<10%

批内差：CV<8%
批间差：CV<10%

注意事项

注意事项

仅供科研使用

仅供科研使用