DTSSP is a water soluble, amine reactive homobifunctional protein crosslinker. This compound is well suited for cell surface protein crossliking, as it is not cell membrane permeable. The disulfide bond of DTSSP can be cleaved with a reducing agent, such as TCEP (CovaChem 11303). pacer Lengths: 12.0 Å Reactive Groups: Sulfo-NHS Esters Reactive Toward: Amino groups (-NH2) CAS Number: 81069-02-5 Alternative Names: DTSSP; Dithiobis (sulfosuccinimidyl propionate) Size Part No. Price 100 mg 13304-100 $158.00 1 gram 13304-1 $959.00
EGS is an amine reactive homobifunctional crosslinker with a moderately long spacer arm. EGS is also available in the water soluble form as Sulfo-EGS (CovaChem 13309). The crosslinks formed by EGS are cleavable by treatment with hydroxylamine (CovaChem 11304). pacer Lengths: 16.1 Å Reactive Groups: NHS Esters Reactive Toward: Amino Groups (-NH2) CAS Number: 70539-42-3 Alternative Names: EGS; D-(N-succinimidyl) ethylene glycol disuccinate; Ethylene glycol disuccinate di(N-succinimidyl) ester Size Part No. Price 100 mg 13308-100 $39.00 1 gram 13308-1 $110.00
1,3-Phenylene dimaleimide (1,3-PDM Crosslinker)
1,3-Phenylene dimaleimide is a thiol reactive homobifunctional protein crosslinker. This crosslinking reagent is particularly advantageous when it is desirable to only modify cysteine residues, which are ordinarily less abundant than lysine residues where amino groups typically reside. pacer Lengths: 9.3 Å Reactive Groups: Maleimides Reactive Toward: Sulfhydryl groups (-SH) CAS Number: 3006-93-7 Alternative Names: m-Phenylene dimaleimide; m-PDM; 1,1’-(1,3-phenylene)bis(1H-pyrrole-2,5-dione) Size Part No. Price 100 mg 13312-100 $109.00 1 gram 13312-1 $209.00
1,4-Phenylene dimaleimide (1,4-PDM Crosslinker)
1,4-Phenylene dimaleimide is a thiol reactive homobifunctional protein crosslinker. This crosslinking reagent is particularly advantageous when it is desirable to only modify cysteine residues, which are ordinarily less abundant than lysine residues where amino groups typically reside. pacer Lengths: 10.2 Å Reactive Groups: Maleimide Reactive Toward: Sulfhydryl Groups (-SH) CAS Number: 3278-31-7 Alternative Names: p-Phenylenedimaleimide; 1,4-Phenylene dimaleimide; p-PDM; 1,1’-(1,4-phenylene)bis(1H-pyrrole-2,5-dione) Size Part No. Price 100 mg 13311-100 $109.00 1 gram 13311-1 $229.00
Sulfo-EGS is a water soluble, amine reactive homobifunctional protein crosslinker. The crosslinks formed by Sulfo-EGS can be easily cleaved by reacting the crosslinked sample with hydroxylamine (CovaChem 11304) at pH 8.5. Spacer Lengths: 16.1 Å Reactive Groups: Sulfo-NHS Esters Reactive Toward: Amino groups (-NH2) Alternative Names: Sulfo-EGS; D-(N-sulfosuccinimidyl) ethylene glycol disuccinate; Ethylene glycol disuccinate di(N-sulfosuccinimidyl) ester Size Part No. Price 100 mg 13309-100 $109.00 1 gram 13309-1 $799.00