重组小鼠β神经生长因子|Mouse beta-NGF|Beta Nerve Growth Factor Mouse Recombinant重组小鼠β神经生长因子|Mouse beta-NGF|Beta Nerve Growth Factor Mouse Recombinant重组小鼠β神经生长因子|Mouse beta-NGF|Beta Nerve Growth Factor Mouse Recombinant重组小鼠β神经生长因子|Mouse beta-NGF|Beta Nerve Growth Factor Mouse Recombinant
ProSpec（蛋白质专家）是以色列一家具有超过16年历史的，专业从事细菌来源重组蛋白生产的先进生物技术公司。ProSpec生产多种细胞因子、生长因子、趋化因子、激素、酶、病毒抗原和许多其他重组蛋白。在研究团队和科学家们的努力下，ProSpec已经拥有超过1700种可用于癌症、细胞凋亡、发育、内分泌学、免疫学、神经科学、蛋白酶及干细胞研究用重组蛋白、多肽和抗体。10多年蛋白纯化方面的经验使ProSpec公司生产成本低、产量高、效率高，其蛋白产品不仅纯度高、生物活性高，价格也非常具有优势。同时ProSpec针对客户的需要提供自定义重组蛋白生产服务。 ProSpec向客户提供蛋白质产品、服务和技术授权。因为创新的产品、卓越的客户服务和对细节的注重，ProSpec在科学界建立了良好的信誉，具有广泛的客户群，包括世界各地的学术、政府研究机构，生物技术和制药企业以及医院和基准实验室。
产品详情：
SYNONYMS
SYNONYMS
Beta Polypeptide, NGF, NGFB, HSAN5, Beta-NGF, MGC161426, MGC161428.
Beta Polypeptide, NGF, NGFB, HSAN5, Beta-NGF, MGC161426, MGC161428.
INTRODUCTION
INTRODUCTION
NGF-beta has nerve growth stimulating activity and the complex is involved in the regulation of growth and the differentiation of sympathetic and certain sensory neurons. Mutations in this gene have been associated with hereditary sensory and autonomic neuropathy, type 5 (HSAN5), and dysregulation of this gene's expression is associated with allergic rhinitis. Nerve Growth Factor was the 1st protein found from the family of neurotrophic factors that influence the growth and differentiation of sympathetic and sensory neurons. NGF consists of 3 different subunits: alpha, beta, and gamma. The beta subunit is accountable for its growth stimulating activity. The synthesis of NGF in astrocytes is enhanced by a range of cytokines such as IL1, TNF-a, PDGF & TGF-b.
NGF-beta has nerve growth stimulating activity and the complex is involved in the regulation of growth and the differentiation of sympathetic and certain sensory neurons. Mutations in this gene have been associated with hereditary sensory and autonomic neuropathy, type 5 (HSAN5), and dysregulation of this gene's expression is associated with allergic rhinitis. Nerve Growth Factor was the 1st protein found from the family of neurotrophic factors that influence the growth and differentiation of sympathetic and sensory neurons. NGF consists of 3 different subunits: alpha, beta, and gamma. The beta subunit is accountable for its growth stimulating activity. The synthesis of NGF in astrocytes is enhanced by a range of cytokines such as IL1, TNF-a, PDGF & TGF-b.
DESCRIPTION
DESCRIPTION
Recombinant Mouse b-NGF produced in E.Coli is a noncovalently disulfide-linked homodimer, non-glycosylated, polypeptide chain containing 2 identical chains of 120 amino acids each and having a molecular mass of 13,471 Dalton each.
The Recombinant Mouse-beta-NGF is purified by advanced biology purification technology.
Recombinant Mouse b-NGF produced in E.Coli is a noncovalently disulfide-linked homodimer, non-glycosylated, polypeptide chain containing 2 identical chains of 120 amino acids each and having a molecular mass of 13,471 Dalton each.
The Recombinant Mouse-beta-NGF is purified by advanced biology purification technology.
SOURCE
SOURCE
Escherichia Coli.
Escherichia Coli.
PHYSICAL APPEARANCE
PHYSICAL APPEARANCE
Sterile Filtered White lyophilized (freeze-dried) powder.
Sterile Filtered White lyophilized (freeze-dried) powder.
FORMULATION
FORMULATION
The Recombinant Mouse b-NGF was lyophilized without additives.
The Recombinant Mouse b-NGF was lyophilized without additives.
SOLUBILITY
SOLUBILITY
It is recommended to reconstitute the lyophilized Murine NGF-beta in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
It is recommended to reconstitute the lyophilized Murine NGF-beta in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
STABILITY
STABILITY
Lyophilized Mouse Beta-NGF although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Murine NGF-Beta should be stored at 4°C between 2-7 days and for future use below -18°C.
Please prevent freeze-thaw cycles.
Lyophilized Mouse Beta-NGF although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Murine NGF-Beta should be stored at 4°C between 2-7 days and for future use below -18°C.
Please prevent freeze-thaw cycles.
PURITY
PURITY
Greater than 98% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE .
Greater than 98% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE .
AMINO ACID SEQUENCE
AMINO ACID SEQUENCE
MSSTHPVFHM GEFSVCDSVS VWVGDKTTAT DIKGKEVTVL AEVNINNSVF RQYFFETKCR ASNPVESGCR GIDSKHWNSY CTTTHTFVKA LTTDEKQAAW RFIRIDTACV CVLSRKATRR G.
MSSTHPVFHM GEFSVCDSVS VWVGDKTTAT DIKGKEVTVL AEVNINNSVF RQYFFETKCR ASNPVESGCR GIDSKHWNSY CTTTHTFVKA LTTDEKQAAW RFIRIDTACV CVLSRKATRR G.
BIOLOGICAL ACTIVITY
BIOLOGICAL ACTIVITY
The Mouse b-NGF activity was measured in a cell proliferation assay using a factor-dependent human erythroleukemic cell line, TF-1, the ED50 for this effect is 0.2 ng/ml, corresponding to a Specific Activity of 5,000,000 units/mg.
The Mouse b-NGF activity was measured in a cell proliferation assay using a factor-dependent human erythroleukemic cell line, TF-1, the ED50 for this effect is 0.2 ng/ml, corresponding to a Specific Activity of 5,000,000 units/mg.
USAGE
USAGE
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
