重组人血清淀粉样蛋白A/Human SAA(APO-SAA)
Recombinant Human Serum Amyloid A (APO-SAA)

INTRODUCTION

INTRODUCTIONSerum amyloid A protein which is a member of the SAA family is encoded by the SAA gene in humans. SAA is expressed by the liver and secreted in plasma. Serum amyloid A is the main acute phase reactant and apolipoprotein of the HDL complex. Elevated serum SAA protein levels may be linked with lung cancer. The level of Apo-SAA, which is ordinarily 1-5 ?g/ml in plasma, increases 500-1000 fold within 24 hours of an inflammatory stimulus and, under these conditions, is the most abundant HDL Apolipoprotein.

DESCRIPTION

DESCRIPTIONAPO-SAA Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 104 amino acids and having a molecular mass of 11.7kDa. Recombinant Apo-SAA is a consensus SAA molecule corresponding to human Apo-SAA1a, except for the presence of an N-terminal methionine, the substitution of asparagine for aspartic acid at position 60, and arginine for histidine at position 71 (the latter two substituted residues are present in Apo-SAA2b).
The Human APO-SAA is purified by proprietary chromatographic techniques.

SOURCE

SOURCEEscherichia Coli.

PHYSICAL APPEARANCE

PHYSICAL APPEARANCESterile Filtered White lyophilized (freeze-dried) powder.

FORMULATION

FORMULATIONHuman SAA was lyophilized from a concentrated (1mg/ml) solution in 20mM Tris-HCl, pH 9.0 and 150mM NaCl.

SOLUBILITY

SOLUBILITYIt is recommended to reconstitute the lyophilized SAA Human in sterile 18M-cm H₂2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

STABILITY

STABILITYLyophilized Human SAA although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Human SAA should be stored at 4°C between 2-7 days and for future use below -18°C.
Please prevent freeze-thaw cycles.

PURITY

PURITYGreater than 98.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.

AMINO ACID SEQUENCE

AMINO ACID SEQUENCERSFFSFLGEA FDGARDMWRA YSDMREANYI GSDKYFHARG NYDAAKRGPG GVWAAEAISN ARENIQRFFG RGAEDSLADQ AANEWGRSGK DPNHFRPAGL PEKY.

BIOLOGICAL ACTIVITY

BIOLOGICAL ACTIVITYThe biological activity determined by its ability to down-regulate lipid biosynthesis in aortic smooth muscle cells. The effective concentration was found to be 4µM.

SAFETY DATA SHEET

SAFETY DATA SHEET

SDS

SDSSDS

USAGE

USAGEProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.