糖原磷酸化酶L(PYGL)重组蛋白Recombinant Glycogen Phosphorylase, Liver (PYGL)
FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!
Organism species Homo sapiens (Human)
Product No. RPA849Hu01
Source Prokaryotic expression
Host E.coli
Purity > 97%
UOM 50ug
Predicted Molecular Mass 22.6kDa
Applications SDS-PAGE; WB; ELISA; IP.
Endotoxin Level <1.0EU per 1µg (determined by the LAL method)
Residues Asn633~Glu820 (Accession # P06737) with N-terminal His-Tag
Formulation Supplied as lyophilized form in PBS, pH7.4, containing 5% 糖原磷酸化酶L(PYGL)重组蛋白trehalose, 0.01% sarcosyl.
SEQUENCES
The target protein is fused with N-terminal His-Tag, its sequence is listed below.
NDPMVGSK LKVIFLENYR VSLAEKVIPA TDLSEQISTA GTEASGTGNM KFMLNGALTI GTMDGANVEM AEEAGEENLF IFGMRIDDVA ALDKKGYEAK EYYEALPELK LVIDQIDNGF FSPKQPDLFK DIINMLFYHD RFKVFADYEA YVKCQDKVSQ LYMNPKAWNT MVLKNIAASG KFSSDRTIKE
USAGE
Reconstitute in sterile PBS, pH7.2-pH7.4.
STORAGE AND STABILITY
Storage: Avoid repeated freeze/thaw cycles. Store at 2-8oC for one month. Aliquot and store at -80oC for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
About the MARKER (complimentary)
Effective Size Range: 10kDa to 70kDa.
Protein bands: 10kDa, 14kDa, 18kDa, 22kDa, 26kDa, 33kDa, 44kDa and 70kDa.
Double intensity bands: The 26kDa, 18kDa, 10kDa bands are at double intensity to make location and size approximation of proteins of interest quick and easy.
糖原磷酸化酶L(PYGL)重组蛋白Ready-to-use: No need to heat, dilute or add reducing agents before use.