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| Immunogen Purified homogenous IgG1 isolated from mouse serum. |
Host Goat |
Clonality Polyclonal |
Purity Affinity purified goat IgG |
Format Lyophilized |
Quantity 1 ml |
Reconstitution For reconstitution add 1.1 ml of sterile water.Let it stand 30 minutes at room temperature to dissolve. Prepare fresh working dilutions daily. |
Storage Stored lyophilize material at 4°C and for long time storage at -20°C. After reconstirution make aliquots and Store at -20°C to avoid repeated freeze-thaw cycles. Prior to use, thaw the aliquot slowly in the dark at ambient temperature, spin down again and prepare the working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Working dilutions should be stored at 4°C, not refrozen, and preferably used the same day. |
Tested applications ELISA (ELISA), Immunocytochemistry (ICC), Immunohistochemistry (IHC)(frozen), Immunofluorescence (IF) |
Recommended dilution 1 : 20-1 :80 The optimum working dilution of each conjugate should be established by titration before being used. Excess labelled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. |
