糜蛋白酶活性测定试剂盒（荧光比色法）价格,详情介绍-960化工网

糜蛋白酶活性测定试剂盒（荧光比色法）

价格：¥5625

品牌：biovision

产地：美国

最新更新日期：2021-06-04 13:59

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联系人：王先生

联系电话： 15210716102

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产品属性

保存条件

-20度

英文名	Chymotrypsin Activity Assay Kit (Fluorometric)

供应商

北京博迈斯

规格

100T

产品说明

Chymotrypsin (EC 3.4.21.1) is a key serine protease involved in dietary protein digestion in mammals. It is primarily produced by the pancreas, but may be expressed in other tissues, including spleen and liver. In the pancreas, chymotrypsin is initially expressed as the inactive proenzyme chymotrypsinogen, which is cleaved by other proteases to chymotrypsin, its active form. Chymotrypsin specifically cleaves peptide bonds at the C-terminal end of bulky hydrophobic or aromatic amino acids (such as tyrosine, tryptophan or phenylalanine). BioVision’s Chymotrypsin Activity Assay kit uses a synthetic fluorogenic substrate, enabling kinetic measurement of chymotrypsin activity in cell and tissue lysates. A chymotrypsin activator cleaves chymotrypsinogen to form active chymotrypsin, which then hydrolyzes the non-fluorescent substrate to release a stable fluorophore (Ex/Em = 380/460 nm). The kit includes a selective chymotrypsin inhibitor that can be used to measure specific chymotrypsin activity in samples containing non-specific proteases and endopeptidases that may also metabolize the substrate. The assay can detect as low as 0.01 mU of Chymotrypsin

Cat # +Size	K352-100
Size	100 assays
Kit Summary	• Detection method- Fluorometric (380/460 nm) • Species reactivity- Mammalian • Application- The assay can detect as low as 0.01 mU of Chymotrypsin in various samples.
Detection Method	Fluorescence (Ex/Em= 380/460 nm)
Species Reactivity	Mammalian
Applications	Measurement of chymotrypsin activity in tissues / cultured cells.
Features & Benefits	• Simple procedure; takes ~ 1-2 hours • Fast and convenient
Kit Components	• Chymotrypsin Assay Buffer • Chymotrypsin Substrate • Chymotrypsin Activator • Chymotrypsin Inhibitor • Coumarin Standard (1 mM) • Chymotrypsin Positive Control
Storage Conditions	-20°C
Shipping Conditions	Gel Pack
USAGE	For Research Use Only! Not For Use in Humans.