适用生物 Mus musculus (Mouse，小鼠)
FMS样酪氨酸激酶3配体(Flt3L)检测试剂盒检测范围 0.781-50pg/mL 灵敏度 0.33pg/mL
样本类型 Serum, plasma and other biological fluids.
实验时长 4.5h 实验方法 双抗夹心法
规格 96T
FMS样酪氨酸激酶3配体(Flt3L)检测试剂盒ELISA Kit for FMS Like Tyrosine Kinase 3 Ligand (Flt3L)
FOR IN VITRO AND RESEARCH USE ONLY, NOT FOR USE IN CLINICAL DIAGNOSTIC PROCEDURES!
| Organism species | Mus musculus (Mouse) |
| Product No. | SEA038Mu |
| Sample type | Serum, plasma and other biological fluids. |
| Format | 96T |
| Assay length | 4.5 hours |
| Detection range | 0.781-50pg/mL The standard curve concentrations used for the ELISA’s were 50pg/mL, 25pg/mL, 12.5pg/mL, 6.25pg/mL, 3.125pg/mL, 1.563pg/mL, 0.781pg/mL |
| Sensitivity | The minimum detectable dose of this kit is typically less than 0.33pg/mL. |
Specificity
This assay has high sensitivity and excellent specificity for detection of FMS Like Tyrosine Kinase 3 Ligand (Flt3L).
No significant cross-reactivity or interference between FMS Like Tyrosine Kinase 3 Ligand (Flt3L) and analogues was observed.
Recovery
Matrices listed below were spiked with certain level of recombinant FMS Like Tyrosine Kinase 3 Ligand (Flt3L) and the recovery rates were calculated by comparing the measured value to the expected amount of FMS Like Tyrosine Kinase 3 Ligand (Flt3L) in samples.
| Matrix | Recovery range (%) | Average(%) |
| serum(n=5) | 87-98 | 91 |
| EDTA plasma(n=5) | 98-105 | 102 |
| heparin plasma(n=5) | 87-95 | 92 |
Precision
FMS样酪氨酸激酶3配体(Flt3L)检测试剂盒Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level FMS Like Tyrosine Kinase 3 Ligand (Flt3L) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level FMS Like Tyrosine Kinase 3 Ligand (Flt3L) were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%
Linearity
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of FMS Like Tyrosine Kinase 3 Ligand (Flt3L) and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
| Sample | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 78-91% | 88-101% | 89-97% | 86-94% |
| EDTA plasma(n=5) | 83-95% | 92-105% | 97-104% | 80-92% |
| heparin plasma(n=5) | 90-97% | 86-94% | 89-98% | 89-103% |
Stability
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Reagents and materials provided
| Reagents | Quantity | Reagents | Quantity |
| Pre-coated, ready to use 96-well strip plate | 1 | Plate sealer for 96 wells | 4 |
| Standard | 2 | Standard Diluent | 1×20mL |
| Detection Reagent A | 1×120µL | Assay Diluent A | 1×12mL |
| Detection Reagent B | 1×120µL | Assay Diluent B | 1×12mL |
| TMB Substrate | 1×9mL | Stop Solution | 1×6mL |
| Wash Buffer (30 × concentrate) | 1×20mL | Instruction manual | 1 |
Assay procedure summary
1. Prepare all reagents, samples and standards;
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37oC;
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37oC;
4. Aspirate and wash 3 times;
5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37oC;
6. Aspirate and wash 5 times;
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37oC;
8. Add 50µL Stop Solution. Read at 450nm immediately.
Test principle
FMS样酪氨酸激酶3配体(Flt3L)检测试剂盒The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to FMS Like Tyrosine Kinase 3 Ligand (Flt3L). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to FMS Like Tyrosine Kinase 3 Ligand (Flt3L). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain FMS Like Tyrosine Kinase 3 Ligand (Flt3L), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of FMS Like Tyrosine Kinase 3 Ligand (Flt3L) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
yb-E13021 猪生长激素释放肽ghrelin(GHRP-Ghrelin)ELISA试剂盒 Porcine growth hormone releasing peptide ghrelin (GHRP-Ghrelin) ELISA Kit
yb-E13022 猪地塞米松(Dex)ELISA试剂盒 Pig dexamethasone (Dex) ELISA kit
yb-E13023 猪D二聚体(D2D)ELISA试剂盒 Pig D -dimer (D2D) ELISA kit
yb-E13024 猪髓过氧化物酶(MPO)ELISA试剂盒 Pig myeloperoxidase (MPO) ELISA kit
yb-E13025 猪超氧化物歧化酶(SOD)ELISA试剂盒 Pig superoxide dismutase (SOD) ELISA kit
yb-E13026 猪血管性血友病因子/瑞斯托霉素辅因子(VWF)ELISA试剂盒 Swine von Willebrand factor / ristocetin cofactor (VWF) ELISA kit
yb-E13027 猪黑色素瘤转移表面黏附分子(MMSAM)ELISA试剂盒 Swine melanoma metastasis surface adhesion molecules (MMSAM) ELISA kit
yb-E13028 猪可溶性血管内皮生长因子受体2(VEGFR-2/sFLK-1)ELISA试剂盒 Pig soluble vascular endothelial growth factor receptor 2 (VEGFR-2/sFLK-1) ELISA kit
yb-E13029 猪血管内皮钙粘着蛋白复合体(VE-cad)ELISA试剂盒 Pig vascular endothelial cadherin complexes (VE-cad) ELISA kit
yb-E13030 猪去甲肾上腺素(NE)ELISA试剂盒 Pig norepinephrine (NE) ELISA kit
yb-E13031 猪血管紧张素Ⅱ(ANG-Ⅱ)ELISA试剂盒 Pig angiotensin Ⅱ (ANG-Ⅱ) ELISA kit
yb-E13032 猪凋亡相关因子(FAS/CD95)ELISA试剂盒 Pig apoptosis-related factors (FAS/CD95) ELISA kit
yb-E13033 猪B细胞淋巴瘤因子2(Bcl-2)ELISA试剂盒 Porcine factor B -cell lymphoma 2 (Bcl-2) ELISA Kit
yb-E13034 猪Bcl-2相关X蛋白(BAX)ELISA试剂盒 Swine associated X protein Bcl-2 (BAX) ELISA kit
yb-E13035 猪(HYD)ELISA试剂盒 Pig hydrocortisone (HYD) ELISA Kit
yb-E13036 猪一氧化氮(NO)ELISA试剂盒 Pig NO (NO) ELISA Kit
yb-E13037 猪内皮型一氧化氮合成酶(eNOS)ELISA试剂盒 Pig endothelial nitric oxide synthase (eNOS) ELISA kit
yb-E13038 猪内皮型一氧化氮合成酶3(eNOS-3)ELISA试剂盒 Pig endothelial nitric oxide synthase 3 (eNOS-3) ELISA kit
yb-E13039 猪血管生成素2(ANG-2)ELISA试剂盒 Pig angiopoietin 2 (ANG-2) ELISA Kit
yb-E13040 猪血管生成素受体/含免疫球蛋白样环和上皮生长因子样域酪氨酸激酶2(Tie-2)ELISA试剂盒 Pig angiopoietin receptor / immunoglobulin-like ring containing epidermal growth factor -like domain and tyrosine kinase 2 (Tie-2) ELISA Kit
yb-E13041 猪血管生成素1(ANG-1)ELISA试剂盒 Pig angiopoietin 1 (ANG-1) ELISA Kit
yb-E13042 猪乙酰胆碱受体抗体(AChRab)ELISA试剂盒 Pig acetylcholine receptor antibody (AChRab) ELISA kit
yb-E13043 猪热休克蛋白90(HSP-90)ELISA试剂盒 Pig heat shock protein 90 (HSP-90) ELISA kit
